Restriction map for
pFN21AE5440
(Linear) (Six-base) MAPSORT of: KIEE5440.seq Check: 9939 from: 1 to: 987
KIEE5440.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AccI GT'mk_AC
Cuts at: 0 95 987
Size: 95 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 786 987
Size: 178 608 201
Fragments arranged by size:
608 201 178
AfeI AGC'GCT
Cuts at: 0 943 987
Size: 943 44
AflII C'TTAA_G
Cuts at: 0 75 987
Size: 75 912
AflIII A'CryG_T
Cuts at: 0 85 987
Size: 85 902
AhdI GACnn_n'nnGTC
Cuts at: 0 441 987
Size: 441 546
AlwNI CAG_nnn'CTG
Cuts at: 0 915 987
Size: 915 72
ApoI r'AATT_y
Cuts at: 0 760 776 987
Size: 760 16 211
Fragments arranged by size:
760 211 16
BamHI G'GATC_C
Cuts at: 0 366 987
Size: 366 621
BbsI GAAGACnn'nnnn_
Cuts at: 0 118 613 987
Size: 118 495 374
Fragments arranged by size:
495 374 118
BciVI GTATCCnnnnn_n'
Cuts at: 0 894 987
Size: 894 93
BclI T'GATC_A
Cuts at: 0 830 987
Size: 830 157
BglII A'GATC_T
Cuts at: 0 346 987
Size: 346 641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 987
Size: 740 247
BsaAI yAC'GTr
Cuts at: 0 86 987
Size: 86 901
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 869 899 987
Size: 37 30 802 30 88
Fragments arranged by size:
802 88 37 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 592 987
Size: 592 395
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 575 987
Size: 524 51 412
Fragments arranged by size:
524 412 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 395 987
Size: 395 592
BsrBI CCG'CTC
Cuts at: 0 969 987
Size: 969 18
BsrFI r'CCGG_y
Cuts at: 0 562 987
Size: 562 425
BsrGI T'GTAC_A
Cuts at: 0 707 987
Size: 707 280
BstAPI GCAn_nnn'nTGC
Cuts at: 0 674 987
Size: 674 313
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 987
Size: 723 264
BstYI r'GATC_y
Cuts at: 0 346 366 474 987
Size: 346 20 108 513
Fragments arranged by size:
513 346 108 20
Bsu36I CC'TnA_GG
Cuts at: 0 362 842 987
Size: 362 480 145
Fragments arranged by size:
480 362 145
BtsI GCAGTG_nn'
Cuts at: 0 587 890 987
Size: 587 303 97
Fragments arranged by size:
587 303 97
EaeI y'GGCC_r
Cuts at: 0 392 483 560 987
Size: 392 91 77 427
Fragments arranged by size:
427 392 91 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 786 987
Size: 178 608 201
Fragments arranged by size:
608 201 178
EcoO109I rG'GnC_Cy
Cuts at: 0 582 987
Size: 582 405
EcoRI G'AATT_C
Cuts at: 0 776 987
Size: 776 211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 493 987
Size: 493 494
HaeII r_GCGC'y
Cuts at: 0 945 987
Size: 945 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 204 236 427 459 520 552
Size: 127 32 45 32 191 32 61 32
Cuts at: 552 987
Size: 435
Fragments arranged by size:
435 191 127 61 45 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 987
Size: 297 690
MscI TGG'CCA
Cuts at: 0 394 485 987
Size: 394 91 502
Fragments arranged by size:
502 394 91
MslI CAynn'nnrTG
Cuts at: 0 721 987
Size: 721 266
NaeI GCC'GGC
Cuts at: 0 564 987
Size: 564 423
NgoMIV G'CCGG_C
Cuts at: 0 562 987
Size: 562 425
NspI r_CATG'y
Cuts at: 0 960 987
Size: 960 27
PasI CC'CwG_GG
Cuts at: 0 639 987
Size: 639 348
PfoI T'CCnGG_A
Cuts at: 0 169 601 987
Size: 169 432 386
Fragments arranged by size:
432 386 169
PmlI CAC'GTG
Cuts at: 0 86 987
Size: 86 901
PstI C_TGCA'G
Cuts at: 0 275 770 987
Size: 275 495 217
Fragments arranged by size:
495 275 217
SfcI C'TryA_G
Cuts at: 0 271 766 987
Size: 271 495 221
Fragments arranged by size:
495 271 221
SmlI C'TyrA_G
Cuts at: 0 75 755 987
Size: 75 680 232
Fragments arranged by size:
680 232 75
StuI AGG'CCT
Cuts at: 0 840 987
Size: 840 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 452 987
Size: 452 535
TatI w'GTAC_w
Cuts at: 0 707 987
Size: 707 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 869 901 987
Size: 37 32 800 32 86
Fragments arranged by size:
800 86 37 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 987
Size: 723 264
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AhdI AlwNI ApoI
BamHI BbsI BciVI BclI BglII BpuEI BsaAI BsaXI
BseRI BsgI BspMI BsrBI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtsI EaeI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I MmeI MscI MslI NaeI NgoMIV NspI
PasI PfoI PmlI PstI SfcI SmlI StuI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BbvCI BcgI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BsrDI BssHII BssSI BstBI
BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI