(Linear) (Six-base) MAPSORT of: KIEE5438.seq  Check: 7979  from: 1  to: 945



KIEE5438.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:00  ..

ApaI G_GGCC'C

Cuts at:      0     416     945 
   Size:        416     529
 
ApoI r'AATT_y

Cuts at:      0      37     945 
   Size:         37     908
 
BamHI G'GATC_C

Cuts at:      0     573     945 
   Size:        573     372
 
BanII G_rGCy'C

Cuts at:      0     416     945 
   Size:        416     529
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     105     813     945 
   Size:        105     708     132

  Fragments arranged by size: 

                 708     132     105
 
BbvCI CC'TCA_GC

Cuts at:      0     499     945 
   Size:        499     446
 
Bme1580I G_kGCm'C

Cuts at:      0     416     945 
   Size:        416     529
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     283     945 
   Size:        271      12     662

  Fragments arranged by size: 

                 662     271      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     499     587     945 
   Size:        499      88     358

  Fragments arranged by size: 

                 499     358      88
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     717     799     945 
   Size:        717      82     146

  Fragments arranged by size: 

                 717     146      82
 
BseYI C'CCAG_C

Cuts at:      0     494     691     803     945 
   Size:        494     197     112     142

  Fragments arranged by size: 

                 494     197     142     112
 
BsmI GAATG_Cn'

Cuts at:      0     340     945 
   Size:        340     605
 
Bsp1286I G_dGCh'C

Cuts at:      0     416     945 
   Size:        416     529
 
BspHI T'CATG_A

Cuts at:      0     767     945 
   Size:        767     178
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     790     945 
   Size:        790     155
 
BstEII G'GTnAC_C

Cuts at:      0     489     843     945 
   Size:        489     354     102

  Fragments arranged by size: 

                 489     354     102
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     666     945 
   Size:        666     279
 
BstYI r'GATC_y

Cuts at:      0     573     945 
   Size:        573     372
 
BtsI GCAGTG_nn'

Cuts at:      0      98     155     239     680     945 
   Size:         98      57      84     441     265

  Fragments arranged by size: 

                 441     265      98      84      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     859     945 
   Size:        859      86
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     283     945 
   Size:        271      12     662

  Fragments arranged by size: 

                 662     271      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     814     945 
   Size:        814     131
 
EcoO109I rG'GnC_Cy

Cuts at:      0     412     945 
   Size:        412     533
 
MspA1I CmG'CkG

Cuts at:      0     331     498     945 
   Size:        331     167     447

  Fragments arranged by size: 

                 447     331     167
 
NdeI CA'TA_TG

Cuts at:      0     360     945 
   Size:        360     585
 
NspI r_CATG'y

Cuts at:      0     725     945 
   Size:        725     220
 
PfoI T'CCnGG_A

Cuts at:      0     209     945 
   Size:        209     736
 
PspOMI G'GGCC_C

Cuts at:      0     412     945 
   Size:        412     533
 
PvuII CAG'CTG

Cuts at:      0     331     498     945 
   Size:        331     167     447

  Fragments arranged by size: 

                 447     331     167
 
SfcI C'TryA_G

Cuts at:      0     740     945 
   Size:        740     205
 
StyI C'CwwG_G

Cuts at:      0      48     815     945 
   Size:         48     767     130

  Fragments arranged by size: 

                 767     130      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     464     496     945 
   Size:        464      32     449

  Fragments arranged by size: 

                 464     449      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     423     666     945 
   Size:        423     243     279

  Fragments arranged by size: 

                 423     279     243
 

 Enzymes that do cut:

     ApaI     ApoI    BamHI    BanII     BbsI    BbvCI Bme1580I     BpmI
   Bpu10I    BseRI    BseYI     BsmI Bsp1286I    BspHI   BstAPI   BstEII
    BstXI    BstYI     BtsI     EarI  Eco57MI    EcoNI EcoO109I   MspA1I
     NdeI     NspI     PfoI   PspOMI    PvuII     SfcI     StyI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI