Restriction map for
pFN21AE5437
(Linear) (Six-base) MAPSORT of: KIEE5437.seq Check: 351 from: 1 to: 1053
KIEE5437.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AflII C'TTAA_G
Cuts at: 0 501 991 1053
Size: 501 490 62
Fragments arranged by size:
501 490 62
AleI CACnn'nnGTG
Cuts at: 0 205 700 1053
Size: 205 495 353
Fragments arranged by size:
495 353 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 1053
Size: 223 830
ApoI r'AATT_y
Cuts at: 0 177 1053
Size: 177 876
AseI AT'TA_AT
Cuts at: 0 458 1053
Size: 458 595
AvaI C'yCGr_G
Cuts at: 0 44 1053
Size: 44 1009
BanI G'GyrC_C
Cuts at: 0 64 1053
Size: 64 989
BbeI G_GCGC'C
Cuts at: 0 68 1053
Size: 68 985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2 36 1053
Size: 2 34 1017
Fragments arranged by size:
1017 34 2
BfrBI ATG'CAT
Cuts at: 0 838 1053
Size: 838 215
BglI GCCn_nnn'nGGC
Cuts at: 0 727 1053
Size: 727 326
Bme1580I G_kGCm'C
Cuts at: 0 212 1053
Size: 212 841
Bpu10I CC'TnA_GC
Cuts at: 0 974 1053
Size: 974 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 943 1053
Size: 286 657 110
Fragments arranged by size:
657 286 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 488 998 1053
Size: 488 510 55
Fragments arranged by size:
510 488 55
BsaAI yAC'GTr
Cuts at: 0 477 1053
Size: 477 576
BsaHI Gr'CG_yC
Cuts at: 0 65 113 1053
Size: 65 48 940
Fragments arranged by size:
940 65 48
BsaWI w'CCGG_w
Cuts at: 0 143 1053
Size: 143 910
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 30 418 421 1053
Size: 25 5 388 3 632
Fragments arranged by size:
632 388 25 5 3
BseYI C'CCAG_C
Cuts at: 0 108 1053
Size: 108 945
BsiEI CG_ry'CG
Cuts at: 0 8 1053
Size: 8 1045
BsiWI C'GTAC_G
Cuts at: 0 477 1053
Size: 477 576
Bsp1286I G_dGCh'C
Cuts at: 0 212 1053
Size: 212 841
BspHI T'CATG_A
Cuts at: 0 507 1053
Size: 507 546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4 1053
Size: 4 1049
BsrDI GCAATG_nn'
Cuts at: 0 836 1053
Size: 836 217
BsrFI r'CCGG_y
Cuts at: 0 67 91 125 1053
Size: 67 24 34 928
Fragments arranged by size:
928 67 34 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 835 1053
Size: 835 218
BstEII G'GTnAC_C
Cuts at: 0 245 1053
Size: 245 808
BstYI r'GATC_y
Cuts at: 0 624 1053
Size: 624 429
BtgI C'CryG_G
Cuts at: 0 824 1053
Size: 824 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 720 755 1053
Size: 720 35 298
Fragments arranged by size:
720 298 35
DraIII CAC_nnn'GTG
Cuts at: 0 569 1053
Size: 569 484
EaeI y'GGCC_r
Cuts at: 0 5 728 1053
Size: 5 723 325
Fragments arranged by size:
723 325 5
EagI C'GGCC_G
Cuts at: 0 5 1053
Size: 5 1048
EarI CTCTTCn'nnn_
Cuts at: 0 344 682 1053
Size: 344 338 371
Fragments arranged by size:
371 344 338
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 1053
Size: 652 401
EcoRV GAT'ATC
Cuts at: 0 257 1053
Size: 257 796
HaeII r_GCGC'y
Cuts at: 0 68 85 1053
Size: 68 17 968
Fragments arranged by size:
968 68 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 362 394 1053
Size: 362 32 659
Fragments arranged by size:
659 362 32
KasI G'GCGC_C
Cuts at: 0 64 1053
Size: 64 989
MfeI C'AATT_G
Cuts at: 0 968 1053
Size: 968 85
MscI TGG'CCA
Cuts at: 0 730 1053
Size: 730 323
MslI CAynn'nnrTG
Cuts at: 0 205 512 700 829 1053
Size: 205 307 188 129 224
Fragments arranged by size:
307 224 205 188 129
MspA1I CmG'CkG
Cuts at: 0 639 1053
Size: 639 414
NaeI GCC'GGC
Cuts at: 0 69 93 1053
Size: 69 24 960
Fragments arranged by size:
960 69 24
NarI GG'CG_CC
Cuts at: 0 65 1053
Size: 65 988
NcoI C'CATG_G
Cuts at: 0 824 1053
Size: 824 229
NgoMIV G'CCGG_C
Cuts at: 0 67 91 1053
Size: 67 24 962
Fragments arranged by size:
962 67 24
NotI GC'GGCC_GC
Cuts at: 0 5 1053
Size: 5 1048
NsiI A_TGCA'T
Cuts at: 0 840 1053
Size: 840 213
PasI CC'CwG_GG
Cuts at: 0 103 1053
Size: 103 950
PshAI GACnn'nnGTC
Cuts at: 0 544 1053
Size: 544 509
PvuII CAG'CTG
Cuts at: 0 639 1053
Size: 639 414
SexAI A'CCwGG_T
Cuts at: 0 760 1053
Size: 760 293
SfoI GGC'GCC
Cuts at: 0 66 1053
Size: 66 987
SgrAI Cr'CCGG_yG
Cuts at: 0 67 1053
Size: 67 986
SmlI C'TyrA_G
Cuts at: 0 301 501 958 991 1053
Size: 301 200 457 33 62
Fragments arranged by size:
457 301 200 62 33
SspI AAT'ATT
Cuts at: 0 772 1053
Size: 772 281
StyI C'CwwG_G
Cuts at: 0 824 1053
Size: 824 229
TatI w'GTAC_w
Cuts at: 0 1017 1053
Size: 1017 36
Enzymes that do cut:
AflII AleI AlfI ApoI AseI AvaI BanI BbeI
BcgI BfrBI BglI Bme1580I Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsiEI BsiWI Bsp1286I BspHI
BspMI BsrDI BsrFI BstAPI BstEII BstYI BtgI CspCI
DraIII EaeI EagI EarI EcoNI EcoRV HaeII Hin4I
KasI MfeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI PasI PshAI PvuII SexAI SfoI
SgrAI SmlI SspI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanII BbsI BbvCI BciVI BclI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BsaBI
BsaXI BsgI BsiHKAI BsmI BsmBI BspEI BsrBI BsrGI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI DraI DrdI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MluI MmeI NdeI NheI NruI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI