Restriction map for
pFN21AE5435
(Linear) (Six-base) MAPSORT of: KIEE5435.seq Check: 5325 from: 1 to: 954
KIEE5435.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 716 802 855 903 913 954
Size: 297 419 86 53 48 10 41
Fragments arranged by size:
419 297 86 53 48 41 10
AfeI AGC'GCT
Cuts at: 0 268 954
Size: 268 686
AhdI GACnn_n'nnGTC
Cuts at: 0 255 954
Size: 255 699
AleI CACnn'nnGTG
Cuts at: 0 742 954
Size: 742 212
AlwNI CAG_nnn'CTG
Cuts at: 0 804 954
Size: 804 150
ApaLI G'TGCA_C
Cuts at: 0 751 954
Size: 751 203
BanII G_rGCy'C
Cuts at: 0 412 954
Size: 412 542
BbsI GAAGACnn'nnnn_
Cuts at: 0 48 916 954
Size: 48 868 38
Fragments arranged by size:
868 48 38
BbvCI CC'TCA_GC
Cuts at: 0 856 954
Size: 856 98
BglI GCCn_nnn'nGGC
Cuts at: 0 283 416 434 954
Size: 283 133 18 520
Fragments arranged by size:
520 283 133 18
Bme1580I G_kGCm'C
Cuts at: 0 755 954
Size: 755 199
BmrI ACTGGGnnnn_n'
Cuts at: 0 792 954
Size: 792 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 168 954
Size: 52 116 786
Fragments arranged by size:
786 116 52
Bpu10I CC'TnA_GC
Cuts at: 0 856 954
Size: 856 98
BsaAI yAC'GTr
Cuts at: 0 369 954
Size: 369 585
BsaBI GATnn'nnATC
Cuts at: 0 826 954
Size: 826 128
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 560 590 954
Size: 560 30 364
Fragments arranged by size:
560 364 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 628 637 954
Size: 628 9 317
Fragments arranged by size:
628 317 9
BseYI C'CCAG_C
Cuts at: 0 117 263 624 954
Size: 117 146 361 330
Fragments arranged by size:
361 330 146 117
BsiEI CG_ry'CG
Cuts at: 0 363 954
Size: 363 591
BsiHKAI G_wGCw'C
Cuts at: 0 404 755 954
Size: 404 351 199
Fragments arranged by size:
404 351 199
BsmBI CGTCTCn'nnnn_
Cuts at: 0 207 563 954
Size: 207 356 391
Fragments arranged by size:
391 356 207
Bsp1286I G_dGCh'C
Cuts at: 0 404 412 755 954
Size: 404 8 343 199
Fragments arranged by size:
404 343 199 8
BspHI T'CATG_A
Cuts at: 0 404 954
Size: 404 550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 260 954
Size: 134 126 694
Fragments arranged by size:
694 134 126
BstEII G'GTnAC_C
Cuts at: 0 474 954
Size: 474 480
BstXI CCAn_nnnn'nTGG
Cuts at: 0 597 954
Size: 597 357
BstYI r'GATC_y
Cuts at: 0 209 518 683 954
Size: 209 309 165 271
Fragments arranged by size:
309 271 209 165
Bsu36I CC'TnA_GG
Cuts at: 0 877 954
Size: 877 77
BtgI C'CryG_G
Cuts at: 0 221 412 954
Size: 221 191 542
Fragments arranged by size:
542 221 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 258 954
Size: 258 696
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 533 568 954
Size: 533 35 386
Fragments arranged by size:
533 386 35
EaeI y'GGCC_r
Cuts at: 0 137 224 245 371 482 587 668 954
Size: 137 87 21 126 111 105 81 286
Fragments arranged by size:
286 137 126 111 105 87 81 21
EarI CTCTTCn'nnn_
Cuts at: 0 85 187 954
Size: 85 102 767
Fragments arranged by size:
767 102 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 168 297 716 802 855 903 913
Size: 52 116 129 419 86 53 48 10
Cuts at: 913 954
Size: 41
Fragments arranged by size:
419 129 116 86 53 52 48 41
10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 930 933 954
Size: 930 3 21
Fragments arranged by size:
930 21 3
HaeII r_GCGC'y
Cuts at: 0 270 954
Size: 270 684
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 241 273 954
Size: 29 212 32 681
Fragments arranged by size:
681 212 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 954
Size: 682 272
MscI TGG'CCA
Cuts at: 0 139 226 373 589 954
Size: 139 87 147 216 365
Fragments arranged by size:
365 216 147 139 87
MslI CAynn'nnrTG
Cuts at: 0 172 349 466 742 820 954
Size: 172 177 117 276 78 134
Fragments arranged by size:
276 177 172 134 117 78
MspA1I CmG'CkG
Cuts at: 0 72 414 954
Size: 72 342 540
Fragments arranged by size:
540 342 72
NspI r_CATG'y
Cuts at: 0 243 954
Size: 243 711
PflMI CCAn_nnn'nTGG
Cuts at: 0 146 804 954
Size: 146 658 150
Fragments arranged by size:
658 150 146
PvuII CAG'CTG
Cuts at: 0 72 954
Size: 72 882
SacII CC_GC'GG
Cuts at: 0 415 954
Size: 415 539
SapI GCTCTTCn'nnn_
Cuts at: 0 85 954
Size: 85 869
SphI G_CATG'C
Cuts at: 0 243 954
Size: 243 711
StuI AGG'CCT
Cuts at: 0 712 954
Size: 712 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 343 954
Size: 277 66 611
Fragments arranged by size:
611 277 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 560 592 954
Size: 560 32 362
Fragments arranged by size:
560 362 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 954
Size: 279 675
XmnI GAAnn'nnTTC
Cuts at: 0 913 954
Size: 913 41
Enzymes that do cut:
AcuI AfeI AhdI AleI AlwNI ApaLI BanII BbsI
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BstEII BstXI BstYI Bsu36I BtgI BtgZI CspCI
EaeI EarI Eco57MI FalI HaeII Hin4I MmeI MscI
MslI MspA1I NspI PflMI PvuII SacII SapI SphI
StuI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaHI BsaWI BsgI BsiWI BsmI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtsI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI
SwaI TatI Tth111I XbaI XhoI XmaI ZraI