(Linear) (Six-base) MAPSORT of: KIEE5432.seq  Check: 7228  from: 1  to: 966



KIEE5432.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     715     966 
   Size:        715     251
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     685     910     966 
   Size:         31     654     225      56

  Fragments arranged by size: 

                 654     225      56      31
 
AleI CACnn'nnGTG

Cuts at:      0     826     966 
   Size:        826     140
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     391     966 
   Size:        391     575
 
ApoI r'AATT_y

Cuts at:      0       3     137     966 
   Size:          3     134     829

  Fragments arranged by size: 

                 829     134       3
 
BamHI G'GATC_C

Cuts at:      0     598     966 
   Size:        598     368
 
BanI G'GyrC_C

Cuts at:      0     686     966 
   Size:        686     280
 
BanII G_rGCy'C

Cuts at:      0     888     966 
   Size:        888      78
 
BclI T'GATC_A

Cuts at:      0      95     966 
   Size:         95     871
 
Bme1580I G_kGCm'C

Cuts at:      0     689     966 
   Size:        689     277
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     103     966 
   Size:        103     863
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     693     966 
   Size:        693     273
 
BsmI GAATG_Cn'

Cuts at:      0     325     966 
   Size:        325     641
 
Bsp1286I G_dGCh'C

Cuts at:      0     689     888     966 
   Size:        689     199      78

  Fragments arranged by size: 

                 689     199      78
 
BspHI T'CATG_A

Cuts at:      0      92     921     966 
   Size:         92     829      45

  Fragments arranged by size: 

                 829      92      45
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     715     966 
   Size:        715     251
 
BsrBI CCG'CTC

Cuts at:      0      31     966 
   Size:         31     935
 
BsrDI GCAATG_nn'

Cuts at:      0     449     966 
   Size:        449     517
 
BsrGI T'GTAC_A

Cuts at:      0     818     966 
   Size:        818     148
 
BstEII G'GTnAC_C

Cuts at:      0     729     966 
   Size:        729     237
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     609     966 
   Size:        609     357
 
BstYI r'GATC_y

Cuts at:      0     598     966 
   Size:        598     368
 
Bsu36I CC'TnA_GG

Cuts at:      0     862     966 
   Size:        862     104
 
BtgI C'CryG_G

Cuts at:      0     338     966 
   Size:        338     628
 
BtsI GCAGTG_nn'

Cuts at:      0     373     966 
   Size:        373     593
 
DraIII CAC_nnn'GTG

Cuts at:      0     620     966 
   Size:        620     346
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     493     886     966 
   Size:         94     399     393      80

  Fragments arranged by size: 

                 399     393      94      80
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     685     910     966 
   Size:         31     654     225      56

  Fragments arranged by size: 

                 654     225      56      31
 
EcoRI G'AATT_C

Cuts at:      0       3     966 
   Size:          3     963
 
EcoRV GAT'ATC

Cuts at:      0     561     966 
   Size:        561     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     266     298     966 
   Size:        266      32     668

  Fragments arranged by size: 

                 668     266      32
 
MslI CAynn'nnrTG

Cuts at:      0     607     763     826     966 
   Size:        607     156      63     140

  Fragments arranged by size: 

                 607     156     140      63
 
MspA1I CmG'CkG

Cuts at:      0     298     966 
   Size:        298     668
 
NcoI C'CATG_G

Cuts at:      0     338     966 
   Size:        338     628
 
NspI r_CATG'y

Cuts at:      0     231     966 
   Size:        231     735
 
SfcI C'TryA_G

Cuts at:      0     855     966 
   Size:        855     111
 
StuI AGG'CCT

Cuts at:      0     697     966 
   Size:        697     269
 
StyI C'CwwG_G

Cuts at:      0     270     338     966 
   Size:        270      68     628

  Fragments arranged by size: 

                 628     270      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     237     966 
   Size:        237     729
 
TatI w'GTAC_w

Cuts at:      0     818     966 
   Size:        818     148
 
XmnI GAAnn'nnTTC

Cuts at:      0     141     966 
   Size:        141     825
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     ApoI    BamHI     BanI    BanII
     BclI Bme1580I     BplI     BsgI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI   DraIII     EarI  Eco57MI    EcoRI    EcoRV    Hin4I     MslI
   MspA1I     NcoI     NspI     SfcI     StuI     StyI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrFI
   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI