(Linear) (Six-base) MAPSORT of: KIEE5427.seq  Check: 2964  from: 1  to: 936



KIEE5427.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:00  ..

AflIII A'CryG_T

Cuts at:      0     517     936 
   Size:        517     419
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     139     936 
   Size:        139     797
 
BanII G_rGCy'C

Cuts at:      0     167     936 
   Size:        167     769
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     148     936 
   Size:        148     788
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     465     936 
   Size:        465     471
 
BclI T'GATC_A

Cuts at:      0     629     936 
   Size:        629     307
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     936 
   Size:         73     863
 
BsaAI yAC'GTr

Cuts at:      0     520     936 
   Size:        520     416
 
BsaBI GATnn'nnATC

Cuts at:      0      76     936 
   Size:         76     860
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      58      88     419     449     936 
   Size:         58      30     331      30     487

  Fragments arranged by size: 

                 487     331      58      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     936 
   Size:        118     818
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     936 
   Size:        167     769
 
BspHI T'CATG_A

Cuts at:      0     159     545     818     936 
   Size:        159     386     273     118

  Fragments arranged by size: 

                 386     273     159     118
 
BsrDI GCAATG_nn'

Cuts at:      0     123     350     362     674     936 
   Size:        123     227      12     312     262

  Fragments arranged by size: 

                 312     262     227     123      12
 
BstYI r'GATC_y

Cuts at:      0      71     936 
   Size:         71     865
 
BtsI GCAGTG_nn'

Cuts at:      0     279     936 
   Size:        279     657
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     712     747     936 
   Size:        712      35     189

  Fragments arranged by size: 

                 712     189      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     643     683     936 
   Size:        643      40     253

  Fragments arranged by size: 

                 643     253      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     895     936 
   Size:        895      41
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     936 
   Size:         73     863
 
MslI CAynn'nnrTG

Cuts at:      0     817     936 
   Size:        817     119
 
NdeI CA'TA_TG

Cuts at:      0     351     936 
   Size:        351     585
 
NspI r_CATG'y

Cuts at:      0     126     936 
   Size:        126     810
 
PmlI CAC'GTG

Cuts at:      0     520     936 
   Size:        520     416
 
PshAI GACnn'nnGTC

Cuts at:      0     152     936 
   Size:        152     784
 
PstI C_TGCA'G

Cuts at:      0      21     936 
   Size:         21     915
 
SfcI C'TryA_G

Cuts at:      0      17     289     867     936 
   Size:         17     272     578      69

  Fragments arranged by size: 

                 578     272      69      17
 
SphI G_CATG'C

Cuts at:      0     126     936 
   Size:        126     810
 
SspI AAT'ATT

Cuts at:      0      12     936 
   Size:         12     924
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     240     936 
   Size:        240     696
 
TatI w'GTAC_w

Cuts at:      0     170     936 
   Size:        170     766
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      58      90     419     451     936 
   Size:         58      32     329      32     485

  Fragments arranged by size: 

                 485     329      58      32      32
 

 Enzymes that do cut:

   AflIII     AlfI    BanII     BbsI    BciVI     BclI     BpmI    BsaAI
    BsaBI    BsaXI    BseRI Bsp1286I    BspHI    BsrDI    BstYI     BtsI
    CspCI     EarI     EciI  Eco57MI     MslI     NdeI     NspI     PmlI
    PshAI     PstI     SfcI     SphI     SspI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI