Restriction map for
pFN21AE5425
(Linear) (Six-base) MAPSORT of: KIEE5425.seq Check: 711 from: 1 to: 933
KIEE5425.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 933
Size: 727 206
AatII G_ACGT'C
Cuts at: 0 890 933
Size: 890 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 933
Size: 696 237
AflIII A'CryG_T
Cuts at: 0 530 933
Size: 530 403
AleI CACnn'nnGTG
Cuts at: 0 115 577 841 933
Size: 115 462 264 92
Fragments arranged by size:
462 264 115 92
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 933
Size: 259 32 642
Fragments arranged by size:
642 259 32
AlwNI CAG_nnn'CTG
Cuts at: 0 719 933
Size: 719 214
ApoI r'AATT_y
Cuts at: 0 12 933
Size: 12 921
AseI AT'TA_AT
Cuts at: 0 862 933
Size: 862 71
BanI G'GyrC_C
Cuts at: 0 843 933
Size: 843 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 812 933
Size: 812 121
BfrBI ATG'CAT
Cuts at: 0 925 933
Size: 925 8
Bme1580I G_kGCm'C
Cuts at: 0 629 848 933
Size: 629 219 85
Fragments arranged by size:
629 219 85
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 33 933
Size: 33 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 933
Size: 651 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 933
Size: 574 359
BsaAI yAC'GTr
Cuts at: 0 105 933
Size: 105 828
BsaHI Gr'CG_yC
Cuts at: 0 887 933
Size: 887 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 393 423 685 715 933
Size: 259 30 104 30 262 30 218
Fragments arranged by size:
262 259 218 104 30 30 30
BseYI C'CCAG_C
Cuts at: 0 460 933
Size: 460 473
BsmI GAATG_Cn'
Cuts at: 0 766 925 933
Size: 766 159 8
Fragments arranged by size:
766 159 8
Bsp1286I G_dGCh'C
Cuts at: 0 629 848 933
Size: 629 219 85
Fragments arranged by size:
629 219 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 689 727 933
Size: 689 38 206
Fragments arranged by size:
689 206 38
BsrDI GCAATG_nn'
Cuts at: 0 356 933
Size: 356 577
BsrGI T'GTAC_A
Cuts at: 0 392 933
Size: 392 541
BstEII G'GTnAC_C
Cuts at: 0 399 933
Size: 399 534
BstYI r'GATC_y
Cuts at: 0 71 933
Size: 71 862
Bsu36I CC'TnA_GG
Cuts at: 0 874 933
Size: 874 59
BtgI C'CryG_G
Cuts at: 0 467 689 933
Size: 467 222 244
Fragments arranged by size:
467 244 222
DraIII CAC_nnn'GTG
Cuts at: 0 482 933
Size: 482 451
EaeI y'GGCC_r
Cuts at: 0 371 933
Size: 371 562
EarI CTCTTCn'nnn_
Cuts at: 0 85 307 933
Size: 85 222 626
Fragments arranged by size:
626 222 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 696 933
Size: 651 45 237
Fragments arranged by size:
651 237 45
EcoRI G'AATT_C
Cuts at: 0 12 933
Size: 12 921
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 927 933
Size: 927 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 685 717 933
Size: 56 32 597 32 216
Fragments arranged by size:
597 216 56 32 32
HincII GTy'rAC
Cuts at: 0 522 933
Size: 522 411
MscI TGG'CCA
Cuts at: 0 373 933
Size: 373 560
MslI CAynn'nnrTG
Cuts at: 0 115 172 577 841 933
Size: 115 57 405 264 92
Fragments arranged by size:
405 264 115 92 57
NcoI C'CATG_G
Cuts at: 0 467 933
Size: 467 466
NsiI A_TGCA'T
Cuts at: 0 927 933
Size: 927 6
NspI r_CATG'y
Cuts at: 0 534 933
Size: 534 399
PciI A'CATG_T
Cuts at: 0 530 933
Size: 530 403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 933
Size: 259 32 642
Fragments arranged by size:
642 259 32
PstI C_TGCA'G
Cuts at: 0 724 933
Size: 724 209
SapI GCTCTTCn'nnn_
Cuts at: 0 307 933
Size: 307 626
SfcI C'TryA_G
Cuts at: 0 720 933
Size: 720 213
SmlI C'TyrA_G
Cuts at: 0 553 933
Size: 553 380
StyI C'CwwG_G
Cuts at: 0 467 933
Size: 467 466
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 427 481 933
Size: 427 54 452
Fragments arranged by size:
452 427 54
TatI w'GTAC_w
Cuts at: 0 176 392 933
Size: 176 216 541
Fragments arranged by size:
541 216 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 20 52 393 425 670 702 933
Size: 20 32 341 32 245 32 231
Fragments arranged by size:
341 245 231 32 32 32 20
ZraI GAC'GTC
Cuts at: 0 888 933
Size: 888 45
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AloI AlwNI ApoI
AseI BanI BbsI BfrBI Bme1580I BplI BpmI BpuEI
BsaAI BsaHI BsaXI BseYI BsmI Bsp1286I BspMI BsrDI
BsrGI BstEII BstYI Bsu36I BtgI DraIII EaeI EarI
Eco57MI EcoRI FalI Hin4I HincII MscI MslI NcoI
NsiI NspI PciI PpiI PstI SapI SfcI SmlI
StyI TaqII TatI TstI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaBI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI