Restriction map for
pFN21AE5424
(Linear) (Six-base) MAPSORT of: KIEE5424.seq Check: 2843 from: 1 to: 963
KIEE5424.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 387 654 963
Size: 251 136 267 309
Fragments arranged by size:
309 267 251 136
ApoI r'AATT_y
Cuts at: 0 162 864 963
Size: 162 702 99
Fragments arranged by size:
702 162 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 963
Size: 464 499
BglI GCCn_nnn'nGGC
Cuts at: 0 56 963
Size: 56 907
BmgBI CAC'GTC
Cuts at: 0 6 963
Size: 6 957
BmrI ACTGGGnnnn_n'
Cuts at: 0 170 830 963
Size: 170 660 133
Fragments arranged by size:
660 170 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 963
Size: 360 603
BseYI C'CCAG_C
Cuts at: 0 395 963
Size: 395 568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 342 465 963
Size: 342 123 498
Fragments arranged by size:
498 342 123
BsrDI GCAATG_nn'
Cuts at: 0 516 963
Size: 516 447
BstAPI GCAn_nnn'nTGC
Cuts at: 0 805 963
Size: 805 158
BtgI C'CryG_G
Cuts at: 0 19 28 100 963
Size: 19 9 72 863
Fragments arranged by size:
863 72 19 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 77 963
Size: 77 886
BtsI GCAGTG_nn'
Cuts at: 0 425 746 780 963
Size: 425 321 34 183
Fragments arranged by size:
425 321 183 34
ClaI AT'CG_AT
Cuts at: 0 153 963
Size: 153 810
DraI TTT'AAA
Cuts at: 0 240 591 963
Size: 240 351 372
Fragments arranged by size:
372 351 240
DrdI GACnn_nn'nnGTC
Cuts at: 0 466 963
Size: 466 497
EaeI y'GGCC_r
Cuts at: 0 97 963
Size: 97 866
EarI CTCTTCn'nnn_
Cuts at: 0 227 963
Size: 227 736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 360 387 654 963
Size: 251 109 27 267 309
Fragments arranged by size:
309 267 251 109 27
EcoRI G'AATT_C
Cuts at: 0 162 864 963
Size: 162 702 99
Fragments arranged by size:
702 162 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 281 721 963
Size: 281 440 242
Fragments arranged by size:
440 281 242
HaeII r_GCGC'y
Cuts at: 0 16 963
Size: 16 947
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 629 661 963
Size: 55 32 542 32 302
Fragments arranged by size:
542 302 55 32 32
MspA1I CmG'CkG
Cuts at: 0 102 963
Size: 102 861
NdeI CA'TA_TG
Cuts at: 0 299 663 963
Size: 299 364 300
Fragments arranged by size:
364 300 299
PfoI T'CCnGG_A
Cuts at: 0 131 963
Size: 131 832
SacII CC_GC'GG
Cuts at: 0 103 963
Size: 103 860
TatI w'GTAC_w
Cuts at: 0 294 963
Size: 294 669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 382 619 963
Size: 382 237 344
Fragments arranged by size:
382 344 237
Enzymes that do cut:
AcuI ApoI BbsI BglI BmgBI BmrI BpmI BseYI
BspMI BsrDI BstAPI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EarI Eco57MI EcoRI FalI HaeII Hin4I
MspA1I NdeI PfoI SacII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I CspCI DraIII EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI