Restriction map for
pFN21AE5423
(Linear) (Six-base) MAPSORT of: KIEE5423.seq Check: 5223 from: 1 to: 1044
KIEE5423.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AccI GT'mk_AC
Cuts at: 0 48 1044
Size: 48 996
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 349 1044
Size: 182 167 695
Fragments arranged by size:
695 182 167
AflIII A'CryG_T
Cuts at: 0 1007 1044
Size: 1007 37
AlwNI CAG_nnn'CTG
Cuts at: 0 42 752 1044
Size: 42 710 292
Fragments arranged by size:
710 292 42
ApaI G_GGCC'C
Cuts at: 0 82 1044
Size: 82 962
ApoI r'AATT_y
Cuts at: 0 487 1044
Size: 487 557
AvaI C'yCGr_G
Cuts at: 0 52 81 1044
Size: 52 29 963
Fragments arranged by size:
963 52 29
BanI G'GyrC_C
Cuts at: 0 31 1044
Size: 31 1013
BanII G_rGCy'C
Cuts at: 0 82 1044
Size: 82 962
BbeI G_GCGC'C
Cuts at: 0 35 1044
Size: 35 1009
BbsI GAAGACnn'nnnn_
Cuts at: 0 112 1044
Size: 112 932
Bme1580I G_kGCm'C
Cuts at: 0 82 1044
Size: 82 962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1044
Size: 740 304
BsaI GGTCTCn'nnnn_
Cuts at: 0 198 708 1044
Size: 198 510 336
Fragments arranged by size:
510 336 198
BsaBI GATnn'nnATC
Cuts at: 0 152 1044
Size: 152 892
BsaHI Gr'CG_yC
Cuts at: 0 32 1044
Size: 32 1012
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 563 1044
Size: 563 481
BseYI C'CCAG_C
Cuts at: 0 42 309 1044
Size: 42 267 735
Fragments arranged by size:
735 267 42
Bsp1286I G_dGCh'C
Cuts at: 0 82 1044
Size: 82 962
BspHI T'CATG_A
Cuts at: 0 653 1044
Size: 653 391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 15 1044
Size: 15 1029
BstYI r'GATC_y
Cuts at: 0 924 1044
Size: 924 120
BtgI C'CryG_G
Cuts at: 0 250 1044
Size: 250 794
DraI TTT'AAA
Cuts at: 0 672 1044
Size: 672 372
DrdI GACnn_nn'nnGTC
Cuts at: 0 382 1044
Size: 382 662
EarI CTCTTCn'nnn_
Cuts at: 0 968 1044
Size: 968 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 349 740 1044
Size: 182 167 391 304
Fragments arranged by size:
391 304 182 167
HaeII r_GCGC'y
Cuts at: 0 35 1044
Size: 35 1009
HincII GTy'rAC
Cuts at: 0 49 1044
Size: 49 995
KasI G'GCGC_C
Cuts at: 0 31 1044
Size: 31 1013
NarI GG'CG_CC
Cuts at: 0 32 1044
Size: 32 1012
NcoI C'CATG_G
Cuts at: 0 250 1044
Size: 250 794
NspI r_CATG'y
Cuts at: 0 352 1044
Size: 352 692
PasI CC'CwG_GG
Cuts at: 0 75 1044
Size: 75 969
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 1044
Size: 42 1002
PfoI T'CCnGG_A
Cuts at: 0 597 1044
Size: 597 447
PshAI GACnn'nnGTC
Cuts at: 0 25 1044
Size: 25 1019
PspOMI G'GGCC_C
Cuts at: 0 78 1044
Size: 78 966
SalI G'TCGA_C
Cuts at: 0 47 1044
Size: 47 997
SexAI A'CCwGG_T
Cuts at: 0 273 1044
Size: 273 771
SfcI C'TryA_G
Cuts at: 0 437 1044
Size: 437 607
SfoI GGC'GCC
Cuts at: 0 33 1044
Size: 33 1011
SmaI CCC'GGG
Cuts at: 0 83 1044
Size: 83 961
StyI C'CwwG_G
Cuts at: 0 250 265 332 1044
Size: 250 15 67 712
Fragments arranged by size:
712 250 67 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 1044
Size: 55 989
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 593 625 800 832 1044
Size: 593 32 175 32 212
Fragments arranged by size:
593 212 175 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 715 1044
Size: 715 329
XmaI C'CCGG_G
Cuts at: 0 81 1044
Size: 81 963
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI Bme1580I BpmI BsaI BsaBI BsaHI
BseRI BseYI Bsp1286I BspHI BspMI BstYI BtgI DraI
DrdI EarI Eco57MI HaeII HincII KasI NarI NcoI
NspI PasI PflMI PfoI PshAI PspOMI SalI SexAI
SfcI SfoI SmaI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaAI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraIII EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI