Restriction map for
pFN21AE5420
(Linear) (Six-base) MAPSORT of: KIEE5420.seq Check: 6694 from: 1 to: 945
KIEE5420.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 667 945
Size: 272 395 278
Fragments arranged by size:
395 278 272
AhdI GACnn_n'nnGTC
Cuts at: 0 397 945
Size: 397 548
ApaLI G'TGCA_C
Cuts at: 0 589 945
Size: 589 356
AvaI C'yCGr_G
Cuts at: 0 490 731 803 945
Size: 490 241 72 142
Fragments arranged by size:
490 241 142 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3 36 945
Size: 3 33 909
Fragments arranged by size:
909 33 3
BanII G_rGCy'C
Cuts at: 0 359 725 945
Size: 359 366 220
Fragments arranged by size:
366 359 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 309 945
Size: 309 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 209 219 243 253 945
Size: 209 10 24 10 692
Fragments arranged by size:
692 209 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 31 945
Size: 31 914
BclI T'GATC_A
Cuts at: 0 32 945
Size: 32 913
Bme1580I G_kGCm'C
Cuts at: 0 88 593 945
Size: 88 505 352
Fragments arranged by size:
505 352 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 945
Size: 294 651
BsaI GGTCTCn'nnnn_
Cuts at: 0 479 728 945
Size: 479 249 217
Fragments arranged by size:
479 249 217
BsaBI GATnn'nnATC
Cuts at: 0 31 945
Size: 31 914
BsaWI w'CCGG_w
Cuts at: 0 692 945
Size: 692 253
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 826 945
Size: 826 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 945
Size: 138 807
BsiEI CG_ry'CG
Cuts at: 0 849 945
Size: 849 96
BsiHKAI G_wGCw'C
Cuts at: 0 593 945
Size: 593 352
Bsp1286I G_dGCh'C
Cuts at: 0 88 359 593 725 945
Size: 88 271 234 132 220
Fragments arranged by size:
271 234 220 132 88
BspEI T'CCGG_A
Cuts at: 0 692 945
Size: 692 253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 205 945
Size: 205 740
BsrDI GCAATG_nn'
Cuts at: 0 74 945
Size: 74 871
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 945
Size: 81 864
BstYI r'GATC_y
Cuts at: 0 727 945
Size: 727 218
BtgI C'CryG_G
Cuts at: 0 824 945
Size: 824 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 546 945
Size: 546 399
BtsI GCAGTG_nn'
Cuts at: 0 86 904 945
Size: 86 818 41
Fragments arranged by size:
818 86 41
ClaI AT'CG_AT
Cuts at: 0 239 687 945
Size: 239 448 258
Fragments arranged by size:
448 258 239
EaeI y'GGCC_r
Cuts at: 0 920 945
Size: 920 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 294 667 945
Size: 272 22 373 278
Fragments arranged by size:
373 278 272 22
EcoO109I rG'GnC_Cy
Cuts at: 0 194 305 807 830 945
Size: 194 111 502 23 115
Fragments arranged by size:
502 194 115 111 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 712 744 945
Size: 383 32 297 32 201
Fragments arranged by size:
383 297 201 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 945
Size: 869 76
MslI CAynn'nnrTG
Cuts at: 0 79 787 945
Size: 79 708 158
Fragments arranged by size:
708 158 79
NcoI C'CATG_G
Cuts at: 0 824 945
Size: 824 121
PpuMI rG'GwC_Cy
Cuts at: 0 194 807 830 945
Size: 194 613 23 115
Fragments arranged by size:
613 194 115 23
PstI C_TGCA'G
Cuts at: 0 435 945
Size: 435 510
SanDI GG'GwC_CC
Cuts at: 0 807 830 945
Size: 807 23 115
Fragments arranged by size:
807 115 23
SfcI C'TryA_G
Cuts at: 0 41 431 678 865 945
Size: 41 390 247 187 80
Fragments arranged by size:
390 247 187 80 41
SmaI CCC'GGG
Cuts at: 0 492 805 945
Size: 492 313 140
Fragments arranged by size:
492 313 140
StyI C'CwwG_G
Cuts at: 0 824 945
Size: 824 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 900 945
Size: 900 45
XmaI C'CCGG_G
Cuts at: 0 490 803 945
Size: 490 313 142
Fragments arranged by size:
490 313 142
Enzymes that do cut:
AcuI AhdI ApaLI AvaI BaeI BanII BbsI BcgI
BciVI BclI Bme1580I BpmI BsaI BsaBI BsaWI BseRI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BstXI
BstYI BtgI BtgZI BtsI ClaI EaeI Eco57MI EcoO109I
Hin4I MmeI MslI NcoI PpuMI PstI SanDI SfcI
SmaI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvrII BamHI BanI BbeI BbvCI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseYI BsiWI BsmI BsmBI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI