(Linear) (Six-base) MAPSORT of: KIEE5406.seq  Check: 7880  from: 1  to: 1071



KIEE5406.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:59  ..

AccI GT'mk_AC

Cuts at:      0     206    1071 
   Size:        206     865
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     755    1071 
   Size:        334     421     316

  Fragments arranged by size: 

                 421     334     316
 
AflIII A'CryG_T

Cuts at:      0     575    1071 
   Size:        575     496
 
AlwNI CAG_nnn'CTG

Cuts at:      0     715    1071 
   Size:        715     356
 
ApaI G_GGCC'C

Cuts at:      0      28    1071 
   Size:         28    1043
 
ApaLI G'TGCA_C

Cuts at:      0     841    1071 
   Size:        841     230
 
ApoI r'AATT_y

Cuts at:      0     462    1071 
   Size:        462     609
 
BamHI G'GATC_C

Cuts at:      0     232    1071 
   Size:        232     839
 
BanII G_rGCy'C

Cuts at:      0      28    1019    1071 
   Size:         28     991      52

  Fragments arranged by size: 

                 991      52      28
 
BbvCI CC'TCA_GC

Cuts at:      0     491    1071 
   Size:        491     580
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     930    1071 
   Size:        930     141
 
BclI T'GATC_A

Cuts at:      0     615    1071 
   Size:        615     456
 
BglI GCCn_nnn'nGGC

Cuts at:      0     980    1071 
   Size:        980      91
 
BlpI GC'TnA_GC

Cuts at:      0     909    1071 
   Size:        909     162
 
Bme1580I G_kGCm'C

Cuts at:      0      28     845    1071 
   Size:         28     817     226

  Fragments arranged by size: 

                 817     226      28
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     506    1071 
   Size:        506     565
 
Bpu10I CC'TnA_GC

Cuts at:      0     240     491    1071 
   Size:        240     251     580

  Fragments arranged by size: 

                 580     251     240
 
BsaAI yAC'GTr

Cuts at:      0     578    1071 
   Size:        578     493
 
BsaHI Gr'CG_yC

Cuts at:      0     118    1071 
   Size:        118     953
 
BsaWI w'CCGG_w

Cuts at:      0     542    1071 
   Size:        542     529
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     486    1071 
   Size:        486     585
 
BsiHKAI G_wGCw'C

Cuts at:      0     845    1071 
   Size:        845     226
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     845    1019    1071 
   Size:         28     817     174      52

  Fragments arranged by size: 

                 817     174      52      28
 
BspEI T'CCGG_A

Cuts at:      0     542    1071 
   Size:        542     529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     671    1071 
   Size:        671     400
 
BsrBI CCG'CTC

Cuts at:      0      82    1071 
   Size:         82     989
 
BsrDI GCAATG_nn'

Cuts at:      0     767    1071 
   Size:        767     304
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      69    1071 
   Size:         69    1002
 
BstEII G'GTnAC_C

Cuts at:      0     878    1071 
   Size:        878     193
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     762    1071 
   Size:        762     309
 
BstYI r'GATC_y

Cuts at:      0     232    1071 
   Size:        232     839
 
BtgI C'CryG_G

Cuts at:      0     395    1071 
   Size:        395     676
 
BtsI GCAGTG_nn'

Cuts at:      0     338     516    1071 
   Size:        338     178     555

  Fragments arranged by size: 

                 555     338     178
 
EaeI y'GGCC_r

Cuts at:      0     793     972    1071 
   Size:        793     179      99

  Fragments arranged by size: 

                 793     179      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     755    1071 
   Size:        334     421     316

  Fragments arranged by size: 

                 421     334     316
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     142     487     660    1071 
   Size:         24     118     345     173     411

  Fragments arranged by size: 

                 411     345     173     118      24
 
EcoRI G'AATT_C

Cuts at:      0     462    1071 
   Size:        462     609
 
EcoRV GAT'ATC

Cuts at:      0     648    1071 
   Size:        648     423
 
HindIII A'AGCT_T

Cuts at:      0     529    1071 
   Size:        529     542
 
MslI CAynn'nnrTG

Cuts at:      0    1010    1071 
   Size:       1010      61
 
MspA1I CmG'CkG

Cuts at:      0      39      60     904    1071 
   Size:         39      21     844     167

  Fragments arranged by size: 

                 844     167      39      21
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     714     746    1071 
   Size:        714      32     325

  Fragments arranged by size: 

                 714     325      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     142     660    1071 
   Size:        142     518     411

  Fragments arranged by size: 

                 518     411     142
 
PspOMI G'GGCC_C

Cuts at:      0      24    1071 
   Size:         24    1047
 
PstI C_TGCA'G

Cuts at:      0     252    1071 
   Size:        252     819
 
PvuII CAG'CTG

Cuts at:      0     904    1071 
   Size:        904     167
 
SexAI A'CCwGG_T

Cuts at:      0     581    1071 
   Size:        581     490
 
SfcI C'TryA_G

Cuts at:      0     248    1071 
   Size:        248     823
 
SspI AAT'ATT

Cuts at:      0     956    1071 
   Size:        956     115
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     275    1071 
   Size:        275     796
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     993    1071 
   Size:        993      78
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI    ApaLI     ApoI    BamHI
    BanII    BbvCI    BciVI     BclI     BglI     BlpI Bme1580I     BplI
   Bpu10I    BsaAI    BsaHI    BsaWI     BsgI  BsiHKAI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI   BstAPI   BstEII    BstXI    BstYI     BtgI
     BtsI     EaeI  Eco57MI EcoO109I    EcoRI    EcoRV  HindIII     MslI
   MspA1I     PpiI    PpuMI   PspOMI     PstI    PvuII    SexAI     SfcI
     SspI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BanI     BbeI     BbsI     BcgI    BfrBI    BglII    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaBI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrFI    BsrGI
   BssHII    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI