Restriction map for
pFN21AE5403
(Linear) (Six-base) MAPSORT of: KIEE5403.seq Check: 5895 from: 1 to: 1020
KIEE5403.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 951 1020
Size: 951 69
AfeI AGC'GCT
Cuts at: 0 245 1020
Size: 245 775
AgeI A'CCGG_T
Cuts at: 0 284 1020
Size: 284 736
AleI CACnn'nnGTG
Cuts at: 0 163 1020
Size: 163 857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 362 1020
Size: 362 658
AlwNI CAG_nnn'CTG
Cuts at: 0 81 1020
Size: 81 939
ApaI G_GGCC'C
Cuts at: 0 19 1020
Size: 19 1001
ApaLI G'TGCA_C
Cuts at: 0 337 1020
Size: 337 683
ApoI r'AATT_y
Cuts at: 0 585 1020
Size: 585 435
AvaI C'yCGr_G
Cuts at: 0 18 256 1020
Size: 18 238 764
Fragments arranged by size:
764 238 18
BanI G'GyrC_C
Cuts at: 0 227 1020
Size: 227 793
BanII G_rGCy'C
Cuts at: 0 19 38 191 1020
Size: 19 19 153 829
Fragments arranged by size:
829 153 19 19
BbeI G_GCGC'C
Cuts at: 0 231 1020
Size: 231 789
BbsI GAAGACnn'nnnn_
Cuts at: 0 400 1020
Size: 400 620
BbvCI CC'TCA_GC
Cuts at: 0 317 1020
Size: 317 703
BciVI GTATCCnnnnn_n'
Cuts at: 0 510 581 1020
Size: 510 71 439
Fragments arranged by size:
510 439 71
BlpI GC'TnA_GC
Cuts at: 0 37 1020
Size: 37 983
Bme1580I G_kGCm'C
Cuts at: 0 19 341 1020
Size: 19 322 679
Fragments arranged by size:
679 322 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 803 1020
Size: 803 217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1020
Size: 418 602
Bpu10I CC'TnA_GC
Cuts at: 0 317 1020
Size: 317 703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1020
Size: 578 442
BsaHI Gr'CG_yC
Cuts at: 0 228 1020
Size: 228 792
BsaWI w'CCGG_w
Cuts at: 0 284 1020
Size: 284 736
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 1020
Size: 199 821
BseYI C'CCAG_C
Cuts at: 0 46 190 1020
Size: 46 144 830
Fragments arranged by size:
830 144 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 690 1020
Size: 109 581 330
Fragments arranged by size:
581 330 109
BsiEI CG_ry'CG
Cuts at: 0 122 164 1020
Size: 122 42 856
Fragments arranged by size:
856 122 42
BsiHKAI G_wGCw'C
Cuts at: 0 38 341 922 1020
Size: 38 303 581 98
Fragments arranged by size:
581 303 98 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 253 1020
Size: 253 767
Bsp1286I G_dGCh'C
Cuts at: 0 19 38 191 341 922 1020
Size: 19 19 153 150 581 98
Fragments arranged by size:
581 153 150 98 19 19
BspHI T'CATG_A
Cuts at: 0 389 1020
Size: 389 631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 951 1020
Size: 951 69
BsrDI GCAATG_nn'
Cuts at: 0 506 1020
Size: 506 514
BsrFI r'CCGG_y
Cuts at: 0 284 1020
Size: 284 736
BsrGI T'GTAC_A
Cuts at: 0 793 816 1020
Size: 793 23 204
Fragments arranged by size:
793 204 23
BstBI TT'CG_AA
Cuts at: 0 687 1020
Size: 687 333
BstYI r'GATC_y
Cuts at: 0 219 1020
Size: 219 801
Bsu36I CC'TnA_GG
Cuts at: 0 142 1020
Size: 142 878
BtgI C'CryG_G
Cuts at: 0 116 1020
Size: 116 904
BtsI GCAGTG_nn'
Cuts at: 0 885 1020
Size: 885 135
EaeI y'GGCC_r
Cuts at: 0 119 161 368 1020
Size: 119 42 207 652
Fragments arranged by size:
652 207 119 42
EagI C'GGCC_G
Cuts at: 0 119 161 1020
Size: 119 42 859
Fragments arranged by size:
859 119 42
EarI CTCTTCn'nnn_
Cuts at: 0 238 1020
Size: 238 782
EcoICRI GAG'CTC
Cuts at: 0 36 1020
Size: 36 984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1020
Size: 418 602
EcoO109I rG'GnC_Cy
Cuts at: 0 205 1020
Size: 205 815
HaeII r_GCGC'y
Cuts at: 0 53 231 247 1020
Size: 53 178 16 773
Fragments arranged by size:
773 178 53 16
HincII GTy'rAC
Cuts at: 0 612 1020
Size: 612 408
KasI G'GCGC_C
Cuts at: 0 227 1020
Size: 227 793
MscI TGG'CCA
Cuts at: 0 370 1020
Size: 370 650
MslI CAynn'nnrTG
Cuts at: 0 163 344 1020
Size: 163 181 676
Fragments arranged by size:
676 181 163
MspA1I CmG'CkG
Cuts at: 0 65 118 1020
Size: 65 53 902
Fragments arranged by size:
902 65 53
NarI GG'CG_CC
Cuts at: 0 228 1020
Size: 228 792
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 1020
Size: 501 519
PsiI TTA'TAA
Cuts at: 0 536 1020
Size: 536 484
PspOMI G'GGCC_C
Cuts at: 0 15 1020
Size: 15 1005
PvuII CAG'CTG
Cuts at: 0 65 1020
Size: 65 955
SacI G_AGCT'C
Cuts at: 0 38 1020
Size: 38 982
SacII CC_GC'GG
Cuts at: 0 119 1020
Size: 119 901
SfcI C'TryA_G
Cuts at: 0 728 1020
Size: 728 292
SfoI GGC'GCC
Cuts at: 0 229 1020
Size: 229 791
SmlI C'TyrA_G
Cuts at: 0 593 1020
Size: 593 427
SspI AAT'ATT
Cuts at: 0 986 1020
Size: 986 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 1020
Size: 292 728
TatI w'GTAC_w
Cuts at: 0 793 816 1020
Size: 793 23 204
Fragments arranged by size:
793 204 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 497 529 1020
Size: 497 32 491
Fragments arranged by size:
497 491 32
Enzymes that do cut:
AarI AfeI AgeI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstBI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII
HincII KasI MscI MslI MspA1I NarI PflMI PsiI
PspOMI PvuII SacI SacII SfcI SfoI SmlI SspI
TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BsaI
BsaAI BsaBI BsaXI BsiWI BsmI BspEI BsrBI BssHII
BssSI BstAPI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI