Restriction map for
pFN21AE5401
(Linear) (Six-base) MAPSORT of: KIEE5401.seq Check: 8587 from: 1 to: 939
KIEE5401.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AccI GT'mk_AC
Cuts at: 0 719 939
Size: 719 220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 939
Size: 127 812
AflIII A'CryG_T
Cuts at: 0 773 939
Size: 773 166
AleI CACnn'nnGTG
Cuts at: 0 316 939
Size: 316 623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 917 939
Size: 917 22
AlwNI CAG_nnn'CTG
Cuts at: 0 432 939
Size: 432 507
ApaI G_GGCC'C
Cuts at: 0 696 874 939
Size: 696 178 65
Fragments arranged by size:
696 178 65
AvaI C'yCGr_G
Cuts at: 0 583 664 939
Size: 583 81 275
Fragments arranged by size:
583 275 81
BanI G'GyrC_C
Cuts at: 0 25 558 939
Size: 25 533 381
Fragments arranged by size:
533 381 25
BanII G_rGCy'C
Cuts at: 0 696 803 874 939
Size: 696 107 71 65
Fragments arranged by size:
696 107 71 65
BbeI G_GCGC'C
Cuts at: 0 29 939
Size: 29 910
BbvCI CC'TCA_GC
Cuts at: 0 420 939
Size: 420 519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 384 418 939
Size: 384 34 521
Fragments arranged by size:
521 384 34
BclI T'GATC_A
Cuts at: 0 308 939
Size: 308 631
BlpI GC'TnA_GC
Cuts at: 0 469 939
Size: 469 470
Bme1580I G_kGCm'C
Cuts at: 0 458 665 696 826 874 939
Size: 458 207 31 130 48 65
Fragments arranged by size:
458 207 130 65 48 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 139 615 939
Size: 103 36 476 324
Fragments arranged by size:
476 324 103 36
Bpu10I CC'TnA_GC
Cuts at: 0 420 939
Size: 420 519
BsaAI yAC'GTr
Cuts at: 0 453 660 774 939
Size: 453 207 114 165
Fragments arranged by size:
453 207 165 114
BsaHI Gr'CG_yC
Cuts at: 0 26 179 939
Size: 26 153 760
Fragments arranged by size:
760 153 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 939
Size: 601 338
BseYI C'CCAG_C
Cuts at: 0 57 213 425 485 875 939
Size: 57 156 212 60 390 64
Fragments arranged by size:
390 212 156 64 60 57
BsiEI CG_ry'CG
Cuts at: 0 206 855 939
Size: 206 649 84
Fragments arranged by size:
649 206 84
BsiHKAI G_wGCw'C
Cuts at: 0 803 939
Size: 803 136
BsiWI C'GTAC_G
Cuts at: 0 38 939
Size: 38 901
BsmBI CGTCTCn'nnnn_
Cuts at: 0 115 939
Size: 115 824
Bsp1286I G_dGCh'C
Cuts at: 0 458 665 696 803 826 874 939
Size: 458 207 31 107 23 48 65
Fragments arranged by size:
458 207 107 65 48 31 23
BsrFI r'CCGG_y
Cuts at: 0 561 939
Size: 561 378
BssHII G'CGCG_C
Cuts at: 0 11 62 939
Size: 11 51 877
Fragments arranged by size:
877 51 11
BstXI CCAn_nnnn'nTGG
Cuts at: 0 648 750 939
Size: 648 102 189
Fragments arranged by size:
648 189 102
BtgI C'CryG_G
Cuts at: 0 317 743 786 939
Size: 317 426 43 153
Fragments arranged by size:
426 317 153 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 354 939
Size: 354 585
BtsI GCAGTG_nn'
Cuts at: 0 436 939
Size: 436 503
DraIII CAC_nnn'GTG
Cuts at: 0 519 939
Size: 519 420
EaeI y'GGCC_r
Cuts at: 0 32 203 678 740 939
Size: 32 171 475 62 199
Fragments arranged by size:
475 199 171 62 32
EagI C'GGCC_G
Cuts at: 0 203 939
Size: 203 736
EarI CTCTTCn'nnn_
Cuts at: 0 295 730 939
Size: 295 435 209
Fragments arranged by size:
435 295 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 237 757 939
Size: 237 520 182
Fragments arranged by size:
520 237 182
EcoICRI GAG'CTC
Cuts at: 0 801 939
Size: 801 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 127 139 615 939
Size: 103 24 12 476 324
Fragments arranged by size:
476 324 103 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 586 700 939
Size: 586 114 239
Fragments arranged by size:
586 239 114
EcoO109I rG'GnC_Cy
Cuts at: 0 489 672 693 871 939
Size: 489 183 21 178 68
Fragments arranged by size:
489 183 178 68 21
HaeII r_GCGC'y
Cuts at: 0 29 432 939
Size: 29 403 507
Fragments arranged by size:
507 403 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 939
Size: 265 32 642
Fragments arranged by size:
642 265 32
KasI G'GCGC_C
Cuts at: 0 25 939
Size: 25 914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 939
Size: 803 136
MscI TGG'CCA
Cuts at: 0 680 742 939
Size: 680 62 197
Fragments arranged by size:
680 197 62
MslI CAynn'nnrTG
Cuts at: 0 316 939
Size: 316 623
MspA1I CmG'CkG
Cuts at: 0 138 939
Size: 138 801
NaeI GCC'GGC
Cuts at: 0 563 939
Size: 563 376
NarI GG'CG_CC
Cuts at: 0 26 939
Size: 26 913
NcoI C'CATG_G
Cuts at: 0 743 939
Size: 743 196
NgoMIV G'CCGG_C
Cuts at: 0 561 939
Size: 561 378
NspI r_CATG'y
Cuts at: 0 477 939
Size: 477 462
PmlI CAC'GTG
Cuts at: 0 453 774 939
Size: 453 321 165
Fragments arranged by size:
453 321 165
PpuMI rG'GwC_Cy
Cuts at: 0 489 939
Size: 489 450
PspOMI G'GGCC_C
Cuts at: 0 692 870 939
Size: 692 178 69
Fragments arranged by size:
692 178 69
PspXI vC'TCGA_Gb
Cuts at: 0 583 939
Size: 583 356
PstI C_TGCA'G
Cuts at: 0 761 939
Size: 761 178
PvuI CG_AT'CG
Cuts at: 0 855 939
Size: 855 84
PvuII CAG'CTG
Cuts at: 0 138 939
Size: 138 801
SacI G_AGCT'C
Cuts at: 0 803 939
Size: 803 136
SanDI GG'GwC_CC
Cuts at: 0 489 939
Size: 489 450
SexAI A'CCwGG_T
Cuts at: 0 50 939
Size: 50 889
SfcI C'TryA_G
Cuts at: 0 757 939
Size: 757 182
SfoI GGC'GCC
Cuts at: 0 27 939
Size: 27 912
SmaI CCC'GGG
Cuts at: 0 666 939
Size: 666 273
SmlI C'TyrA_G
Cuts at: 0 583 939
Size: 583 356
SphI G_CATG'C
Cuts at: 0 477 939
Size: 477 462
StuI AGG'CCT
Cuts at: 0 361 592 939
Size: 361 231 347
Fragments arranged by size:
361 347 231
StyI C'CwwG_G
Cuts at: 0 29 743 939
Size: 29 714 196
Fragments arranged by size:
714 196 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 645 939
Size: 645 294
XhoI C'TCGA_G
Cuts at: 0 583 939
Size: 583 356
XmaI C'CCGG_G
Cuts at: 0 664 939
Size: 664 275
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbvCI BcgI BclI BlpI Bme1580I
BpmI Bpu10I BsaAI BsaHI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BsrFI BssHII BstXI BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PmlI PpuMI
PspOMI PspXI PstI PvuI PvuII SacI SanDI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BciVI BfrBI BglI BglII BmgBI BmrI
BmtI BplI BpuEI BsaI BsaBI BsaWI BsaXI BsgI
BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI RsrII SacII SalI
SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XmnI ZraI