Restriction map for
pFN21AE5396
(Linear) (Six-base) MAPSORT of: KIEE5396.seq Check: 1391 from: 1 to: 927
KIEE5396.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 927
Size: 178 749
AflIII A'CryG_T
Cuts at: 0 709 720 927
Size: 709 11 207
Fragments arranged by size:
709 207 11
AleI CACnn'nnGTG
Cuts at: 0 250 927
Size: 250 677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 76 927
Size: 76 851
ApaI G_GGCC'C
Cuts at: 0 506 927
Size: 506 421
BanI G'GyrC_C
Cuts at: 0 4 927
Size: 4 923
BanII G_rGCy'C
Cuts at: 0 506 927
Size: 506 421
BglII A'GATC_T
Cuts at: 0 326 927
Size: 326 601
Bme1580I G_kGCm'C
Cuts at: 0 506 927
Size: 506 421
BmtI G_CTAG'C
Cuts at: 0 427 927
Size: 427 500
Bpu10I CC'TnA_GC
Cuts at: 0 181 915 927
Size: 181 734 12
Fragments arranged by size:
734 181 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 927
Size: 532 395
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 927
Size: 586 341
BsaAI yAC'GTr
Cuts at: 0 723 927
Size: 723 204
BsaBI GATnn'nnATC
Cuts at: 0 536 927
Size: 536 391
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 924 927
Size: 466 458 3
Fragments arranged by size:
466 458 3
BseYI C'CCAG_C
Cuts at: 0 290 927
Size: 290 637
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 581 927
Size: 367 214 346
Fragments arranged by size:
367 346 214
BsiHKAI G_wGCw'C
Cuts at: 0 104 927
Size: 104 823
BsmI GAATG_Cn'
Cuts at: 0 456 927
Size: 456 471
Bsp1286I G_dGCh'C
Cuts at: 0 104 506 927
Size: 104 402 421
Fragments arranged by size:
421 402 104
BsrFI r'CCGG_y
Cuts at: 0 689 927
Size: 689 238
BsrGI T'GTAC_A
Cuts at: 0 764 927
Size: 764 163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 313 786 927
Size: 313 473 141
Fragments arranged by size:
473 313 141
BstYI r'GATC_y
Cuts at: 0 326 927
Size: 326 601
BtgI C'CryG_G
Cuts at: 0 344 927
Size: 344 583
DrdI GACnn_nn'nnGTC
Cuts at: 0 159 927
Size: 159 768
EaeI y'GGCC_r
Cuts at: 0 365 593 927
Size: 365 228 334
Fragments arranged by size:
365 334 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 927
Size: 178 749
EcoO109I rG'GnC_Cy
Cuts at: 0 377 431 927
Size: 377 54 496
Fragments arranged by size:
496 377 54
MscI TGG'CCA
Cuts at: 0 367 595 927
Size: 367 228 332
Fragments arranged by size:
367 332 228
MslI CAynn'nnrTG
Cuts at: 0 250 571 589 760 823 927
Size: 250 321 18 171 63 104
Fragments arranged by size:
321 250 171 104 63 18
MspA1I CmG'CkG
Cuts at: 0 489 602 927
Size: 489 113 325
Fragments arranged by size:
489 325 113
NcoI C'CATG_G
Cuts at: 0 344 927
Size: 344 583
NdeI CA'TA_TG
Cuts at: 0 205 927
Size: 205 722
NheI G'CTAG_C
Cuts at: 0 423 927
Size: 423 504
NspI r_CATG'y
Cuts at: 0 74 713 927
Size: 74 639 214
Fragments arranged by size:
639 214 74
PciI A'CATG_T
Cuts at: 0 709 927
Size: 709 218
PspOMI G'GGCC_C
Cuts at: 0 502 927
Size: 502 425
PstI C_TGCA'G
Cuts at: 0 470 927
Size: 470 457
PvuII CAG'CTG
Cuts at: 0 489 927
Size: 489 438
SfcI C'TryA_G
Cuts at: 0 372 466 927
Size: 372 94 461
Fragments arranged by size:
461 372 94
SmlI C'TyrA_G
Cuts at: 0 547 927
Size: 547 380
StyI C'CwwG_G
Cuts at: 0 344 927
Size: 344 583
TatI w'GTAC_w
Cuts at: 0 764 927
Size: 764 163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 313 927
Size: 313 614
Enzymes that do cut:
AcuI AflIII AleI AlfI ApaI BanI BanII BglII
Bme1580I BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BsrFI BsrGI BstXI
BstYI BtgI DrdI EaeI Eco57MI EcoO109I MscI MslI
MspA1I NcoI NdeI NheI NspI PciI PspOMI PstI
PvuII SfcI SmlI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmrI BplI
BpmI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NgoMIV NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI