(Linear) (Six-base) MAPSORT of: KIEE5395.seq  Check: 9681  from: 1  to: 927



KIEE5395.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     812     927 
   Size:        812     115
 
AleI CACnn'nnGTG

Cuts at:      0     109     927 
   Size:        109     818
 
ApoI r'AATT_y

Cuts at:      0     232     927 
   Size:        232     695
 
BamHI G'GATC_C

Cuts at:      0      51     927 
   Size:         51     876
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     609     927 
   Size:        609     318
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     927 
   Size:        520     407
 
Bpu10I CC'TnA_GC

Cuts at:      0     181     927 
   Size:        181     746
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     687     927 
   Size:        687     240
 
BsaAI yAC'GTr

Cuts at:      0     363     927 
   Size:        363     564
 
BseYI C'CCAG_C

Cuts at:      0     441     927 
   Size:        441     486
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     543     927 
   Size:         82     461     384

  Fragments arranged by size: 

                 461     384      82
 
BsmI GAATG_Cn'

Cuts at:      0     870     927 
   Size:        870      57
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     759     927 
   Size:        759     168
 
BsrDI GCAATG_nn'

Cuts at:      0     123     927 
   Size:        123     804
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     111     273     313     927 
   Size:        111     162      40     614

  Fragments arranged by size: 

                 614     162     111      40
 
BstYI r'GATC_y

Cuts at:      0      51     326     927 
   Size:         51     275     601

  Fragments arranged by size: 

                 601     275      51
 
BtgI C'CryG_G

Cuts at:      0     104     927 
   Size:        104     823
 
EaeI y'GGCC_r

Cuts at:      0       2     365     593     927 
   Size:          2     363     228     334

  Fragments arranged by size: 

                 363     334     228       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     386     927 
   Size:        386     541
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     812     927 
   Size:        520     292     115

  Fragments arranged by size: 

                 520     292     115
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      46     927 
   Size:         46     881
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     817     927 
   Size:        817     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     740     772     927 
   Size:        740      32     155

  Fragments arranged by size: 

                 740     155      32
 
HincII GTy'rAC

Cuts at:      0     409     663     927 
   Size:        409     254     264

  Fragments arranged by size: 

                 409     264     254
 
HindIII A'AGCT_T

Cuts at:      0     550     927 
   Size:        550     377
 
MfeI C'AATT_G

Cuts at:      0     487     927 
   Size:        487     440
 
MscI TGG'CCA

Cuts at:      0       4     367     595     927 
   Size:          4     363     228     332

  Fragments arranged by size: 

                 363     332     228       4
 
MslI CAynn'nnrTG

Cuts at:      0     109     571     634     727     832     927 
   Size:        109     462      63      93     105      95

  Fragments arranged by size: 

                 462     109     105      95      93      63
 
MspA1I CmG'CkG

Cuts at:      0      58     927 
   Size:         58     869
 
NspI r_CATG'y

Cuts at:      0     771     927 
   Size:        771     156
 
PfoI T'CCnGG_A

Cuts at:      0      47     927 
   Size:         47     880
 
PstI C_TGCA'G

Cuts at:      0     684     927 
   Size:        684     243
 
SfcI C'TryA_G

Cuts at:      0     680     927 
   Size:        680     247
 
SmlI C'TyrA_G

Cuts at:      0     666     927 
   Size:        666     261
 
StyI C'CwwG_G

Cuts at:      0     194     927 
   Size:        194     733
 
TatI w'GTAC_w

Cuts at:      0       7     170     927 
   Size:          7     163     757

  Fragments arranged by size: 

                 757     163       7
 

 Enzymes that do cut:

     AcuI     AleI     ApoI    BamHI     BbsI     BpmI   Bpu10I    BpuEI
    BsaAI    BseYI     BsgI     BsmI    BspMI    BsrDI    BstXI    BstYI
     BtgI     EaeI     EarI  Eco57MI    EcoNI     FalI    Hin4I   HincII
  HindIII     MfeI     MscI     MslI   MspA1I     NspI     PfoI     PstI
     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI