Restriction map for
pFN21AE5394
(Linear) (Six-base) MAPSORT of: KIEE5394.seq Check: 1371 from: 1 to: 927
KIEE5394.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AccI GT'mk_AC
Cuts at: 0 860 927
Size: 860 67
AleI CACnn'nnGTG
Cuts at: 0 575 927
Size: 575 352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 387 927
Size: 387 540
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 927
Size: 50 32 845
Fragments arranged by size:
845 50 32
ApoI r'AATT_y
Cuts at: 0 919 927
Size: 919 8
AvrII C'CTAG_G
Cuts at: 0 429 927
Size: 429 498
BanI G'GyrC_C
Cuts at: 0 415 927
Size: 415 512
BbsI GAAGACnn'nnnn_
Cuts at: 0 735 927
Size: 735 192
BbvCI CC'TCA_GC
Cuts at: 0 216 927
Size: 216 711
BciVI GTATCCnnnnn_n'
Cuts at: 0 202 927
Size: 202 725
BclI T'GATC_A
Cuts at: 0 833 927
Size: 833 94
BfrBI ATG'CAT
Cuts at: 0 280 678 892 927
Size: 280 398 214 35
Fragments arranged by size:
398 280 214 35
BglI GCCn_nnn'nGGC
Cuts at: 0 346 424 927
Size: 346 78 503
Fragments arranged by size:
503 346 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 927
Size: 768 159
Bpu10I CC'TnA_GC
Cuts at: 0 216 686 927
Size: 216 470 241
Fragments arranged by size:
470 241 216
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 248 278 927
Size: 248 30 649
Fragments arranged by size:
649 248 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 204 269 927
Size: 91 113 65 658
Fragments arranged by size:
658 113 91 65
BseYI C'CCAG_C
Cuts at: 0 241 778 927
Size: 241 537 149
Fragments arranged by size:
537 241 149
BsmI GAATG_Cn'
Cuts at: 0 678 892 894 927
Size: 678 214 2 33
Fragments arranged by size:
678 214 33 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 927
Size: 237 690
BstYI r'GATC_y
Cuts at: 0 65 927
Size: 65 862
Bsu36I CC'TnA_GG
Cuts at: 0 868 927
Size: 868 59
BtgI C'CryG_G
Cuts at: 0 317 794 927
Size: 317 477 133
Fragments arranged by size:
477 317 133
BtsI GCAGTG_nn'
Cuts at: 0 374 728 927
Size: 374 354 199
Fragments arranged by size:
374 354 199
DraIII CAC_nnn'GTG
Cuts at: 0 110 927
Size: 110 817
EaeI y'GGCC_r
Cuts at: 0 314 404 927
Size: 314 90 523
Fragments arranged by size:
523 314 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 927
Size: 768 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 248 280 927
Size: 248 32 647
Fragments arranged by size:
647 248 32
HindIII A'AGCT_T
Cuts at: 0 603 927
Size: 603 324
MscI TGG'CCA
Cuts at: 0 316 927
Size: 316 611
MslI CAynn'nnrTG
Cuts at: 0 166 397 575 841 927
Size: 166 231 178 266 86
Fragments arranged by size:
266 231 178 166 86
MspA1I CmG'CkG
Cuts at: 0 220 927
Size: 220 707
NcoI C'CATG_G
Cuts at: 0 794 927
Size: 794 133
NdeI CA'TA_TG
Cuts at: 0 438 927
Size: 438 489
NsiI A_TGCA'T
Cuts at: 0 282 680 894 927
Size: 282 398 214 33
Fragments arranged by size:
398 282 214 33
NspI r_CATG'y
Cuts at: 0 284 927
Size: 284 643
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 927
Size: 795 132
PshAI GACnn'nnGTC
Cuts at: 0 152 927
Size: 152 775
PstI C_TGCA'G
Cuts at: 0 735 927
Size: 735 192
PvuII CAG'CTG
Cuts at: 0 220 927
Size: 220 707
SfcI C'TryA_G
Cuts at: 0 61 731 861 927
Size: 61 670 130 66
Fragments arranged by size:
670 130 66 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 346 927
Size: 346 581
SpeI A'CTAG_T
Cuts at: 0 574 927
Size: 574 353
StyI C'CwwG_G
Cuts at: 0 230 311 429 794 927
Size: 230 81 118 365 133
Fragments arranged by size:
365 230 133 118 81
TatI w'GTAC_w
Cuts at: 0 170 927
Size: 170 757
Tth111I GACn'n_nGTC
Cuts at: 0 262 927
Size: 262 665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 927
Size: 792 135
XmnI GAAnn'nnTTC
Cuts at: 0 892 927
Size: 892 35
Enzymes that do cut:
AccI AleI AlfI AloI ApoI AvrII BanI BbsI
BbvCI BciVI BclI BfrBI BglI BpmI Bpu10I BsaXI
BseRI BseYI BsmI BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI Eco57MI Hin4I HindIII MscI MslI MspA1I
NcoI NdeI NsiI NspI PflMI PshAI PstI PvuII
SfcI SfiI SpeI StyI TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanII BbeI BcgI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI TaqII TstI
XbaI XhoI XmaI ZraI