Restriction map for
pFN21AE5393
(Linear) (Six-base) MAPSORT of: KIEE5393.seq Check: 5582 from: 1 to: 927
KIEE5393.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 927
Size: 922 5
AflIII A'CryG_T
Cuts at: 0 653 718 927
Size: 653 65 209
Fragments arranged by size:
653 209 65
ApoI r'AATT_y
Cuts at: 0 249 927
Size: 249 678
BbsI GAAGACnn'nnnn_
Cuts at: 0 705 819 927
Size: 705 114 108
Fragments arranged by size:
705 114 108
BbvCI CC'TCA_GC
Cuts at: 0 692 927
Size: 692 235
BciVI GTATCCnnnnn_n'
Cuts at: 0 147 927
Size: 147 780
BclI T'GATC_A
Cuts at: 0 839 927
Size: 839 88
BfrBI ATG'CAT
Cuts at: 0 286 684 927
Size: 286 398 243
Fragments arranged by size:
398 286 243
BmgBI CAC'GTC
Cuts at: 0 719 927
Size: 719 208
BmrI ACTGGGnnnn_n'
Cuts at: 0 310 927
Size: 310 617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 859 927
Size: 395 464 68
Fragments arranged by size:
464 395 68
Bpu10I CC'TnA_GC
Cuts at: 0 692 927
Size: 692 235
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 541 927
Size: 541 386
BsmI GAATG_Cn'
Cuts at: 0 422 684 927
Size: 422 262 243
Fragments arranged by size:
422 262 243
BspHI T'CATG_A
Cuts at: 0 320 927
Size: 320 607
BsrBI CCG'CTC
Cuts at: 0 151 927
Size: 151 776
BsrGI T'GTAC_A
Cuts at: 0 257 927
Size: 257 670
BstYI r'GATC_y
Cuts at: 0 593 927
Size: 593 334
Bsu36I CC'TnA_GG
Cuts at: 0 553 676 927
Size: 553 123 251
Fragments arranged by size:
553 251 123
BtgI C'CryG_G
Cuts at: 0 350 927
Size: 350 577
EaeI y'GGCC_r
Cuts at: 0 371 927
Size: 371 556
EarI CTCTTCn'nnn_
Cuts at: 0 112 225 927
Size: 112 113 702
Fragments arranged by size:
702 113 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 481 927
Size: 481 446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 859 922 927
Size: 395 464 63 5
Fragments arranged by size:
464 395 63 5
EcoO109I rG'GnC_Cy
Cuts at: 0 758 927
Size: 758 169
EcoRV GAT'ATC
Cuts at: 0 326 927
Size: 326 601
HincII GTy'rAC
Cuts at: 0 522 927
Size: 522 405
MfeI C'AATT_G
Cuts at: 0 12 927
Size: 12 915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 927
Size: 536 391
MscI TGG'CCA
Cuts at: 0 373 927
Size: 373 554
MslI CAynn'nnrTG
Cuts at: 0 172 319 769 847 927
Size: 172 147 450 78 80
Fragments arranged by size:
450 172 147 80 78
MspA1I CmG'CkG
Cuts at: 0 691 927
Size: 691 236
NcoI C'CATG_G
Cuts at: 0 350 927
Size: 350 577
NsiI A_TGCA'T
Cuts at: 0 288 686 927
Size: 288 398 241
Fragments arranged by size:
398 288 241
NspI r_CATG'y
Cuts at: 0 290 546 657 735 927
Size: 290 256 111 78 192
Fragments arranged by size:
290 256 192 111 78
PciI A'CATG_T
Cuts at: 0 653 927
Size: 653 274
PflMI CCAn_nnn'nTGG
Cuts at: 0 801 927
Size: 801 126
PfoI T'CCnGG_A
Cuts at: 0 411 927
Size: 411 516
PpuMI rG'GwC_Cy
Cuts at: 0 758 927
Size: 758 169
PvuII CAG'CTG
Cuts at: 0 691 927
Size: 691 236
SanDI GG'GwC_CC
Cuts at: 0 758 927
Size: 758 169
SfcI C'TryA_G
Cuts at: 0 450 867 927
Size: 450 417 60
Fragments arranged by size:
450 417 60
StyI C'CwwG_G
Cuts at: 0 350 927
Size: 350 577
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 34 249 377 927
Size: 34 215 128 550
Fragments arranged by size:
550 215 128 34
TatI w'GTAC_w
Cuts at: 0 176 257 773 927
Size: 176 81 516 154
Fragments arranged by size:
516 176 154 81
XmnI GAAnn'nnTTC
Cuts at: 0 709 927
Size: 709 218
Enzymes that do cut:
AcuI AflIII ApoI BbsI BbvCI BciVI BclI BfrBI
BmgBI BmrI BpmI Bpu10I BseRI BsmI BspHI BsrBI
BsrGI BstYI Bsu36I BtgI EaeI EarI EciI Eco57MI
EcoO109I EcoRV HincII MfeI MmeI MscI MslI MspA1I
NcoI NsiI NspI PciI PflMI PfoI PpuMI PvuII
SanDI SfcI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BcgI BglI BglII BlpI Bme1580I BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI