Restriction map for
pFN21AE5391
(Linear) (Six-base) MAPSORT of: KIEE5391.seq Check: 4245 from: 1 to: 924
KIEE5391.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AleI CACnn'nnGTG
Cuts at: 0 115 622 838 924
Size: 115 507 216 86
Fragments arranged by size:
507 216 115 86
AvrII C'CTAG_G
Cuts at: 0 255 924
Size: 255 669
BanI G'GyrC_C
Cuts at: 0 624 924
Size: 624 300
BanII G_rGCy'C
Cuts at: 0 68 924
Size: 68 856
BbvCI CC'TCA_GC
Cuts at: 0 187 329 924
Size: 187 142 595
Fragments arranged by size:
595 187 142
Bme1580I G_kGCm'C
Cuts at: 0 629 924
Size: 629 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 924
Size: 52 872
Bpu10I CC'TnA_GC
Cuts at: 0 187 329 924
Size: 187 142 595
Fragments arranged by size:
595 187 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 802 832 924
Size: 670 30 102 30 92
Fragments arranged by size:
670 102 92 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 541 678 924
Size: 541 137 246
Fragments arranged by size:
541 246 137
BsiHKAI G_wGCw'C
Cuts at: 0 68 924
Size: 68 856
Bsp1286I G_dGCh'C
Cuts at: 0 68 629 924
Size: 68 561 295
Fragments arranged by size:
561 295 68
BsrBI CCG'CTC
Cuts at: 0 686 924
Size: 686 238
BsrDI GCAATG_nn'
Cuts at: 0 602 924
Size: 602 322
BsrFI r'CCGG_y
Cuts at: 0 698 924
Size: 698 226
BstEII G'GTnAC_C
Cuts at: 0 334 924
Size: 334 590
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 924
Size: 243 681
BtgI C'CryG_G
Cuts at: 0 5 350 836 924
Size: 5 345 486 88
Fragments arranged by size:
486 345 88 5
BtsI GCAGTG_nn'
Cuts at: 0 385 424 924
Size: 385 39 500
Fragments arranged by size:
500 385 39
EarI CTCTTCn'nnn_
Cuts at: 0 85 924
Size: 85 839
EcoICRI GAG'CTC
Cuts at: 0 66 924
Size: 66 858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 924
Size: 52 872
EcoNI CCTnn'n_nnAGG
Cuts at: 0 805 924
Size: 805 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 802 834 924
Size: 685 32 85 32 90
Fragments arranged by size:
685 90 85 32 32
HindIII A'AGCT_T
Cuts at: 0 707 924
Size: 707 217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 924
Size: 593 331
MslI CAynn'nnrTG
Cuts at: 0 115 172 479 581 622 739 838 924
Size: 115 57 307 102 41 117 99 86
Fragments arranged by size:
307 117 115 102 99 86 57 41
MspA1I CmG'CkG
Cuts at: 0 72 924
Size: 72 852
NcoI C'CATG_G
Cuts at: 0 5 350 924
Size: 5 345 574
Fragments arranged by size:
574 345 5
NdeI CA'TA_TG
Cuts at: 0 357 924
Size: 357 567
NspI r_CATG'y
Cuts at: 0 722 924
Size: 722 202
PsiI TTA'TAA
Cuts at: 0 395 924
Size: 395 529
PvuII CAG'CTG
Cuts at: 0 72 924
Size: 72 852
SacI G_AGCT'C
Cuts at: 0 68 924
Size: 68 856
SfcI C'TryA_G
Cuts at: 0 378 666 924
Size: 378 288 258
Fragments arranged by size:
378 288 258
StyI C'CwwG_G
Cuts at: 0 5 255 350 620 924
Size: 5 250 95 270 304
Fragments arranged by size:
304 270 250 95 5
TatI w'GTAC_w
Cuts at: 0 176 924
Size: 176 748
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 89 121 670 702 924
Size: 89 32 549 32 222
Fragments arranged by size:
549 222 89 32 32
Enzymes that do cut:
AleI AvrII BanI BanII BbvCI Bme1580I BpmI Bpu10I
BsaXI BseRI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstEII
BstXI BtgI BtsI EarI EcoICRI Eco57MI EcoNI Hin4I
HindIII MmeI MslI MspA1I NcoI NdeI NspI PsiI
PvuII SacI SfcI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BaeI BamHI BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI