Restriction map for
pFN21AE5388
(Linear) (Six-base) MAPSORT of: KIEE5388.seq Check: 3320 from: 1 to: 924
KIEE5388.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AccI GT'mk_AC
Cuts at: 0 217 924
Size: 217 707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 924
Size: 52 872
AleI CACnn'nnGTG
Cuts at: 0 739 838 924
Size: 739 99 86
Fragments arranged by size:
739 99 86
BamHI G'GATC_C
Cuts at: 0 680 924
Size: 680 244
BanII G_rGCy'C
Cuts at: 0 442 924
Size: 442 482
BclI T'GATC_A
Cuts at: 0 809 924
Size: 809 115
Bme1580I G_kGCm'C
Cuts at: 0 236 476 924
Size: 236 240 448
Fragments arranged by size:
448 240 236
BmgBI CAC'GTC
Cuts at: 0 777 825 924
Size: 777 48 99
Fragments arranged by size:
777 99 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 52 265 924
Size: 52 213 659
Fragments arranged by size:
659 213 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 830 924
Size: 226 604 94
Fragments arranged by size:
604 226 94
BsaAI yAC'GTr
Cuts at: 0 719 924
Size: 719 205
BsaBI GATnn'nnATC
Cuts at: 0 103 924
Size: 103 821
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 924
Size: 199 30 695
Fragments arranged by size:
695 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 714 924
Size: 714 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 549 924
Size: 309 240 375
Fragments arranged by size:
375 309 240
BsiHKAI G_wGCw'C
Cuts at: 0 151 724 746 924
Size: 151 573 22 178
Fragments arranged by size:
573 178 151 22
Bsp1286I G_dGCh'C
Cuts at: 0 151 236 442 476 724 746 924
Size: 151 85 206 34 248 22 178
Fragments arranged by size:
248 206 178 151 85 34 22
BspHI T'CATG_A
Cuts at: 0 404 924
Size: 404 520
BsrDI GCAATG_nn'
Cuts at: 0 464 539 924
Size: 464 75 385
Fragments arranged by size:
464 385 75
BsrGI T'GTAC_A
Cuts at: 0 101 656 924
Size: 101 555 268
Fragments arranged by size:
555 268 101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 255 924
Size: 243 12 669
Fragments arranged by size:
669 243 12
BstYI r'GATC_y
Cuts at: 0 680 804 924
Size: 680 124 120
Fragments arranged by size:
680 124 120
BstZ17I GTA'TAC
Cuts at: 0 218 924
Size: 218 706
Bsu36I CC'TnA_GG
Cuts at: 0 676 924
Size: 676 248
BtgI C'CryG_G
Cuts at: 0 350 737 924
Size: 350 387 187
Fragments arranged by size:
387 350 187
BtsI GCAGTG_nn'
Cuts at: 0 236 476 924
Size: 236 240 448
Fragments arranged by size:
448 240 236
DraI TTT'AAA
Cuts at: 0 854 924
Size: 854 70
EaeI y'GGCC_r
Cuts at: 0 245 461 696 924
Size: 245 216 235 228
Fragments arranged by size:
245 235 228 216
EarI CTCTTCn'nnn_
Cuts at: 0 71 924
Size: 71 853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 226 830 924
Size: 52 174 604 94
Fragments arranged by size:
604 174 94 52
MscI TGG'CCA
Cuts at: 0 247 463 924
Size: 247 216 461
Fragments arranged by size:
461 247 216
MslI CAynn'nnrTG
Cuts at: 0 469 739 838 924
Size: 469 270 99 86
Fragments arranged by size:
469 270 99 86
MspA1I CmG'CkG
Cuts at: 0 365 429 924
Size: 365 64 495
Fragments arranged by size:
495 365 64
NcoI C'CATG_G
Cuts at: 0 350 924
Size: 350 574
PasI CC'CwG_GG
Cuts at: 0 435 924
Size: 435 489
PfoI T'CCnGG_A
Cuts at: 0 203 924
Size: 203 721
PmlI CAC'GTG
Cuts at: 0 719 924
Size: 719 205
PshAI GACnn'nnGTC
Cuts at: 0 365 924
Size: 365 559
PvuII CAG'CTG
Cuts at: 0 429 924
Size: 429 495
ScaI AGT'ACT
Cuts at: 0 334 924
Size: 334 590
StyI C'CwwG_G
Cuts at: 0 350 800 924
Size: 350 450 124
Fragments arranged by size:
450 350 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 924
Size: 435 489
TatI w'GTAC_w
Cuts at: 0 101 176 302 332 656 924
Size: 101 75 126 30 324 268
Fragments arranged by size:
324 268 126 101 75 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 265 924
Size: 233 32 659
Fragments arranged by size:
659 233 32
Enzymes that do cut:
AccI AcuI AleI BamHI BanII BclI Bme1580I BmgBI
BmrI BpmI BsaAI BsaBI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BspHI BsrDI BsrGI BstXI BstYI BstZ17I Bsu36I
BtgI BtsI DraI EaeI EarI Eco57MI MscI MslI
MspA1I NcoI PasI PfoI PmlI PshAI PvuII ScaI
StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaI BsaHI BsaWI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PpiI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI