(Linear) (Six-base) MAPSORT of: KIEE5385.seq  Check: 6436  from: 1  to: 921



KIEE5385.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:58  ..

AflII C'TTAA_G

Cuts at:      0     873     921 
   Size:        873      48
 
AflIII A'CryG_T

Cuts at:      0     653     718     921 
   Size:        653      65     203

  Fragments arranged by size: 

                 653     203      65
 
AleI CACnn'nnGTG

Cuts at:      0     115     921 
   Size:        115     806
 
AlwNI CAG_nnn'CTG

Cuts at:      0     417     468     921 
   Size:        417      51     453

  Fragments arranged by size: 

                 453     417      51
 
ApoI r'AATT_y

Cuts at:      0      31     249     331     921 
   Size:         31     218      82     590

  Fragments arranged by size: 

                 590     218      82      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      81     921 
   Size:         81     840
 
BclI T'GATC_A

Cuts at:      0     134     921 
   Size:        134     787
 
BfrBI ATG'CAT

Cuts at:      0     286     684     921 
   Size:        286     398     237

  Fragments arranged by size: 

                 398     286     237
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     109     921 
   Size:        109     812
 
Bpu10I CC'TnA_GC

Cuts at:      0      62     921 
   Size:         62     859
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     764     921 
   Size:        764     157
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     615     921 
   Size:        615     306
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     921 
   Size:        278     643
 
BsmI GAATG_Cn'

Cuts at:      0     304     437     684     921 
   Size:        304     133     247     237

  Fragments arranged by size: 

                 304     247     237     133
 
BsrGI T'GTAC_A

Cuts at:      0     773     921 
   Size:        773     148
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     292     921 
   Size:        292     629
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     243     921 
   Size:        243     678
 
BtgI C'CryG_G

Cuts at:      0     350     921 
   Size:        350     571
 
EaeI y'GGCC_r

Cuts at:      0     371     921 
   Size:        371     550
 
EarI CTCTTCn'nnn_

Cuts at:      0     772     878     921 
   Size:        772     106      43

  Fragments arranged by size: 

                 772     106      43
 
EcoRI G'AATT_C

Cuts at:      0      31     921 
   Size:         31     890
 
MscI TGG'CCA

Cuts at:      0     373     921 
   Size:        373     548
 
MslI CAynn'nnrTG

Cuts at:      0      40     115     775     921 
   Size:         40      75     660     146

  Fragments arranged by size: 

                 660     146      75      40
 
MspA1I CmG'CkG

Cuts at:      0     300     921 
   Size:        300     621
 
NcoI C'CATG_G

Cuts at:      0     350     921 
   Size:        350     571
 
NsiI A_TGCA'T

Cuts at:      0     288     686     921 
   Size:        288     398     235

  Fragments arranged by size: 

                 398     288     235
 
NspI r_CATG'y

Cuts at:      0     290     657     722     780     921 
   Size:        290     367      65      58     141

  Fragments arranged by size: 

                 367     290     141      65      58
 
PciI A'CATG_T

Cuts at:      0     653     718     921 
   Size:        653      65     203

  Fragments arranged by size: 

                 653     203      65
 
PstI C_TGCA'G

Cuts at:      0      71     693     921 
   Size:         71     622     228

  Fragments arranged by size: 

                 622     228      71
 
PvuII CAG'CTG

Cuts at:      0     300     921 
   Size:        300     621
 
SfcI C'TryA_G

Cuts at:      0      67     450     689     921 
   Size:         67     383     239     232

  Fragments arranged by size: 

                 383     239     232      67
 
SmlI C'TyrA_G

Cuts at:      0     873     921 
   Size:        873      48
 
StyI C'CwwG_G

Cuts at:      0     236     350     921 
   Size:        236     114     571

  Fragments arranged by size: 

                 571     236     114
 
TatI w'GTAC_w

Cuts at:      0     176     426     773     921 
   Size:        176     250     347     148

  Fragments arranged by size: 

                 347     250     176     148
 
Tth111I GACn'n_nGTC

Cuts at:      0     419     921 
   Size:        419     502
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     921 
   Size:         35     886
 

 Enzymes that do cut:

    AflII   AflIII     AleI    AlwNI     ApoI     BbsI     BclI    BfrBI
     BplI   Bpu10I     BsaI    BseRI     BsgI     BsmI    BsrGI   BstAPI
    BstXI     BtgI     EaeI     EarI    EcoRI     MscI     MslI   MspA1I
     NcoI     NsiI     NspI     PciI     PstI    PvuII     SfcI     SmlI
     StyI     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     ZraI