(Linear) (Six-base) MAPSORT of: KIEE5383.seq  Check: 6149  from: 1  to: 921



KIEE5383.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     921 
   Size:        343     578
 
AflIII A'CryG_T

Cuts at:      0       6     494     574     921 
   Size:          6     488      80     347

  Fragments arranged by size: 

                 488     347      80       6
 
ApoI r'AATT_y

Cuts at:      0     161     333     921 
   Size:        161     172     588

  Fragments arranged by size: 

                 588     172     161
 
BbvCI CC'TCA_GC

Cuts at:      0     304     921 
   Size:        304     617
 
BlpI GC'TnA_GC

Cuts at:      0     147     921 
   Size:        147     774
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     921 
   Size:        304     617
 
BsaAI yAC'GTr

Cuts at:      0       7     921 
   Size:          7     914
 
BsaWI w'CCGG_w

Cuts at:      0     229     921 
   Size:        229     692
 
BspHI T'CATG_A

Cuts at:      0     392     921 
   Size:        392     529
 
BsrDI GCAATG_nn'

Cuts at:      0     437     680     921 
   Size:        437     243     241

  Fragments arranged by size: 

                 437     243     241
 
BsrFI r'CCGG_y

Cuts at:      0     142     921 
   Size:        142     779
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     439     921 
   Size:        439     482
 
BstEII G'GTnAC_C

Cuts at:      0     232     921 
   Size:        232     689
 
BstYI r'GATC_y

Cuts at:      0     486     921 
   Size:        486     435
 
EaeI y'GGCC_r

Cuts at:      0     728     921 
   Size:        728     193
 
EarI CTCTTCn'nnn_

Cuts at:      0     358     921 
   Size:        358     563
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     921 
   Size:        343     578
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     864     921 
   Size:        864      57
 
EcoRV GAT'ATC

Cuts at:      0     689     921 
   Size:        689     232
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     195     227     921 
   Size:        195      32     694

  Fragments arranged by size: 

                 694     195      32
 
MfeI C'AATT_G

Cuts at:      0     880     921 
   Size:        880      41
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     674     921 
   Size:        365     309     247

  Fragments arranged by size: 

                 365     309     247
 
MscI TGG'CCA

Cuts at:      0     730     921 
   Size:        730     191
 
NspI r_CATG'y

Cuts at:      0     498     578     921 
   Size:        498      80     343

  Fragments arranged by size: 

                 498     343      80
 
PasI CC'CwG_GG

Cuts at:      0     809     921 
   Size:        809     112
 
PciI A'CATG_T

Cuts at:      0     494     574     921 
   Size:        494      80     347

  Fragments arranged by size: 

                 494     347      80
 
PsiI TTA'TAA

Cuts at:      0     461     921 
   Size:        461     460
 
PstI C_TGCA'G

Cuts at:      0     878     921 
   Size:        878      43
 
SapI GCTCTTCn'nnn_

Cuts at:      0     358     921 
   Size:        358     563
 
SfcI C'TryA_G

Cuts at:      0     874     921 
   Size:        874      47
 
Tth111I GACn'n_nGTC

Cuts at:      0     478     921 
   Size:        478     443
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI    BbvCI     BlpI   Bpu10I    BsaAI    BsaWI
    BspHI    BsrDI    BsrFI   BstAPI   BstEII    BstYI     EaeI     EarI
  Eco57MI    EcoNI    EcoRV    Hin4I     MfeI     MmeI     MscI     NspI
     PasI     PciI     PsiI     PstI     SapI     SfcI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrGI
   BssHII    BssSI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI