(Linear) (Six-base) MAPSORT of: KIEE5382.seq  Check: 8932  from: 1  to: 915



KIEE5382.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:01  ..

AccI GT'mk_AC

Cuts at:      0     244     915 
   Size:        244     671
 
AleI CACnn'nnGTG

Cuts at:      0     835     915 
   Size:        835      80
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     915 
   Size:         75     840
 
ApoI r'AATT_y

Cuts at:      0      31     915 
   Size:         31     884
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     560     593     915 
   Size:        560      33     322

  Fragments arranged by size: 

                 560     322      33
 
BanII G_rGCy'C

Cuts at:      0      50     915 
   Size:         50     865
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     271     915 
   Size:        271     644
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     603     915 
   Size:         52     551     312

  Fragments arranged by size: 

                 551     312      52
 
BsaWI w'CCGG_w

Cuts at:      0     674     843     915 
   Size:        674     169      72

  Fragments arranged by size: 

                 674     169      72
 
BsmI GAATG_Cn'

Cuts at:      0     706     915 
   Size:        706     209
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     915 
   Size:         50     865
 
BspEI T'CCGG_A

Cuts at:      0     674     915 
   Size:        674     241
 
BsrBI CCG'CTC

Cuts at:      0     546     915 
   Size:        546     369
 
BsrDI GCAATG_nn'

Cuts at:      0     308     353     915 
   Size:        308      45     562

  Fragments arranged by size: 

                 562     308      45
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     639     915 
   Size:        639     276
 
BstYI r'GATC_y

Cuts at:      0     505     915 
   Size:        505     410
 
BtsI GCAGTG_nn'

Cuts at:      0      24     915 
   Size:         24     891
 
EaeI y'GGCC_r

Cuts at:      0     368     428     915 
   Size:        368      60     487

  Fragments arranged by size: 

                 487     368      60
 
EarI CTCTTCn'nnn_

Cuts at:      0     472     915 
   Size:        472     443
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     478     915 
   Size:        478     437
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     603     915 
   Size:         52     551     312

  Fragments arranged by size: 

                 551     312      52
 
EcoRI G'AATT_C

Cuts at:      0      31     915 
   Size:         31     884
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     912     915 
   Size:        912       3
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     404     436     497     529     852     884     915 
   Size:        404      32      61      32     323      32      31

  Fragments arranged by size: 

                 404     323      61      32      32      32      31
 
HincII GTy'rAC

Cuts at:      0     245     915 
   Size:        245     670
 
HindIII A'AGCT_T

Cuts at:      0     553     915 
   Size:        553     362
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     915 
   Size:         29     886
 
MscI TGG'CCA

Cuts at:      0     370     430     915 
   Size:        370      60     485

  Fragments arranged by size: 

                 485     370      60
 
MslI CAynn'nnrTG

Cuts at:      0     136     172     835     915 
   Size:        136      36     663      80

  Fragments arranged by size: 

                 663     136      80      36
 
NdeI CA'TA_TG

Cuts at:      0     728     915 
   Size:        728     187
 
NspI r_CATG'y

Cuts at:      0     339     589     771     915 
   Size:        339     250     182     144

  Fragments arranged by size: 

                 339     250     182     144
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     732     915 
   Size:        732     183
 
PfoI T'CCnGG_A

Cuts at:      0     406     915 
   Size:        406     509
 
SalI G'TCGA_C

Cuts at:      0     243     915 
   Size:        243     672
 
SfcI C'TryA_G

Cuts at:      0     390     501     864     915 
   Size:        390     111     363      51

  Fragments arranged by size: 

                 390     363     111      51
 
SphI G_CATG'C

Cuts at:      0     589     915 
   Size:        589     326
 
StuI AGG'CCT

Cuts at:      0     895     915 
   Size:        895      20
 
TatI w'GTAC_w

Cuts at:      0     176     915 
   Size:        176     739
 

 Enzymes that do cut:

     AccI     AleI    AlwNI     ApoI     BaeI    BanII     BbsI     BpmI
    BsaWI     BsmI Bsp1286I    BspEI    BsrBI    BsrDI    BstXI    BstYI
     BtsI     EaeI     EarI     EciI  Eco57MI    EcoRI     FalI    Hin4I
   HincII  HindIII     MmeI     MscI     MslI     NdeI     NspI    PflMI
     PfoI     SalI     SfcI     SphI     StuI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI