(Linear) (Six-base) MAPSORT of: KIEE5381.seq  Check: 8531  from: 1  to: 915



KIEE5381.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     915 
   Size:        185     730
 
AhdI GACnn_n'nnGTC

Cuts at:      0     739     915 
   Size:        739     176
 
ApaLI G'TGCA_C

Cuts at:      0     469     915 
   Size:        469     446
 
ApoI r'AATT_y

Cuts at:      0      10     784     915 
   Size:         10     774     131

  Fragments arranged by size: 

                 774     131      10
 
AvrII C'CTAG_G

Cuts at:      0     523     915 
   Size:        523     392
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     110     143     915 
   Size:        110      33     772

  Fragments arranged by size: 

                 772     110      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     191     868     915 
   Size:        191     677      47

  Fragments arranged by size: 

                 677     191      47
 
BclI T'GATC_A

Cuts at:      0     809     915 
   Size:        809     106
 
Bme1580I G_kGCm'C

Cuts at:      0     473     915 
   Size:        473     442
 
BmtI G_CTAG'C

Cuts at:      0     169     915 
   Size:        169     746
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     915 
   Size:         31     884
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31     915 
   Size:         31     884
 
BsaBI GATnn'nnATC

Cuts at:      0     316     915 
   Size:        316     599
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      77     107     915 
   Size:         77      30     808

  Fragments arranged by size: 

                 808      77      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     473     596     915 
   Size:        473     123     319

  Fragments arranged by size: 

                 473     319     123
 
Bsp1286I G_dGCh'C

Cuts at:      0     473     596     915 
   Size:        473     123     319

  Fragments arranged by size: 

                 473     319     123
 
BsrDI GCAATG_nn'

Cuts at:      0     575     657     915 
   Size:        575      82     258

  Fragments arranged by size: 

                 575     258      82
 
BsrGI T'GTAC_A

Cuts at:      0     544     915 
   Size:        544     371
 
BtgI C'CryG_G

Cuts at:      0     329     915 
   Size:        329     586
 
DraIII CAC_nnn'GTG

Cuts at:      0     602     915 
   Size:        602     313
 
EaeI y'GGCC_r

Cuts at:      0     757     915 
   Size:        757     158
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     185     915 
   Size:         31     154     730

  Fragments arranged by size: 

                 730     154      31
 
EcoRI G'AATT_C

Cuts at:      0      10     915 
   Size:         10     905
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     661     693     725     757     801     833     915 
   Size:        661      32      32      32      44      32      82

  Fragments arranged by size: 

                 661      82      44      32      32      32      32
 
MscI TGG'CCA

Cuts at:      0     759     915 
   Size:        759     156
 
MslI CAynn'nnrTG

Cuts at:      0     121     328     643     902     915 
   Size:        121     207     315     259      13

  Fragments arranged by size: 

                 315     259     207     121      13
 
NcoI C'CATG_G

Cuts at:      0     329     915 
   Size:        329     586
 
NheI G'CTAG_C

Cuts at:      0     165     915 
   Size:        165     750
 
NspI r_CATG'y

Cuts at:      0     401     915 
   Size:        401     514
 
SfcI C'TryA_G

Cuts at:      0     555     915 
   Size:        555     360
 
StyI C'CwwG_G

Cuts at:      0     329     523     915 
   Size:        329     194     392

  Fragments arranged by size: 

                 392     329     194
 
TatI w'GTAC_w

Cuts at:      0     155     544     915 
   Size:        155     389     371

  Fragments arranged by size: 

                 389     371     155
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      77     109     915 
   Size:         77      32     806

  Fragments arranged by size: 

                 806      77      32
 

 Enzymes that do cut:

     AcuI     AhdI    ApaLI     ApoI    AvrII     BaeI     BbsI     BclI
 Bme1580I     BmtI     BpmI     BsaI    BsaBI    BsaXI  BsiHKAI Bsp1286I
    BsrDI    BsrGI     BtgI   DraIII     EaeI  Eco57MI    EcoRI    Hin4I
     MscI     MslI     NcoI     NheI     NspI     SfcI     StyI     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BplI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI