Restriction map for
pFN21AE5375
(Linear) (Six-base) MAPSORT of: KIEE5375.seq Check: 8778 from: 1 to: 909
KIEE5375.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 381 479 909
Size: 381 98 430
Fragments arranged by size:
430 381 98
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 909
Size: 686 223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 461 909
Size: 461 448
ApaI G_GGCC'C
Cuts at: 0 734 909
Size: 734 175
ApoI r'AATT_y
Cuts at: 0 7 99 909
Size: 7 92 810
Fragments arranged by size:
810 92 7
BanI G'GyrC_C
Cuts at: 0 586 909
Size: 586 323
BanII G_rGCy'C
Cuts at: 0 664 734 909
Size: 664 70 175
Fragments arranged by size:
664 175 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 732 909
Size: 732 177
BbvCI CC'TCA_GC
Cuts at: 0 163 364 909
Size: 163 201 545
Fragments arranged by size:
545 201 163
BclI T'GATC_A
Cuts at: 0 494 599 909
Size: 494 105 310
Fragments arranged by size:
494 310 105
BfrBI ATG'CAT
Cuts at: 0 438 909
Size: 438 471
BglII A'GATC_T
Cuts at: 0 185 225 909
Size: 185 40 684
Fragments arranged by size:
684 185 40
Bme1580I G_kGCm'C
Cuts at: 0 734 909
Size: 734 175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 909
Size: 678 231
Bpu10I CC'TnA_GC
Cuts at: 0 163 364 909
Size: 163 201 545
Fragments arranged by size:
545 201 163
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 186 909
Size: 186 723
BseYI C'CCAG_C
Cuts at: 0 93 909
Size: 93 816
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 909
Size: 64 845
BsiHKAI G_wGCw'C
Cuts at: 0 116 664 909
Size: 116 548 245
Fragments arranged by size:
548 245 116
BsmI GAATG_Cn'
Cuts at: 0 438 909
Size: 438 471
Bsp1286I G_dGCh'C
Cuts at: 0 116 664 734 909
Size: 116 548 70 175
Fragments arranged by size:
548 175 116 70
BspHI T'CATG_A
Cuts at: 0 122 380 909
Size: 122 258 529
Fragments arranged by size:
529 258 122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 381 479 909
Size: 381 98 430
Fragments arranged by size:
430 381 98
BsrGI T'GTAC_A
Cuts at: 0 77 909
Size: 77 832
BstYI r'GATC_y
Cuts at: 0 185 225 909
Size: 185 40 684
Fragments arranged by size:
684 185 40
DraIII CAC_nnn'GTG
Cuts at: 0 345 909
Size: 345 564
EaeI y'GGCC_r
Cuts at: 0 347 909
Size: 347 562
EarI CTCTTCn'nnn_
Cuts at: 0 481 865 909
Size: 481 384 44
Fragments arranged by size:
481 384 44
EcoICRI GAG'CTC
Cuts at: 0 662 909
Size: 662 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 686 909
Size: 678 8 223
Fragments arranged by size:
678 223 8
EcoO109I rG'GnC_Cy
Cuts at: 0 612 698 731 909
Size: 612 86 33 178
Fragments arranged by size:
612 178 86 33
EcoRI G'AATT_C
Cuts at: 0 7 909
Size: 7 902
MfeI C'AATT_G
Cuts at: 0 86 909
Size: 86 823
MscI TGG'CCA
Cuts at: 0 349 909
Size: 349 560
MslI CAynn'nnrTG
Cuts at: 0 121 325 379 909
Size: 121 204 54 530
Fragments arranged by size:
530 204 121 54
NsiI A_TGCA'T
Cuts at: 0 440 909
Size: 440 469
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 909
Size: 345 564
PpuMI rG'GwC_Cy
Cuts at: 0 612 698 909
Size: 612 86 211
Fragments arranged by size:
612 211 86
PspOMI G'GGCC_C
Cuts at: 0 730 909
Size: 730 179
SacI G_AGCT'C
Cuts at: 0 664 909
Size: 664 245
SanDI GG'GwC_CC
Cuts at: 0 698 909
Size: 698 211
SapI GCTCTTCn'nnn_
Cuts at: 0 481 909
Size: 481 428
SfcI C'TryA_G
Cuts at: 0 203 909
Size: 203 706
StuI AGG'CCT
Cuts at: 0 756 909
Size: 756 153
StyI C'CwwG_G
Cuts at: 0 138 909
Size: 138 771
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 411 909
Size: 411 498
TatI w'GTAC_w
Cuts at: 0 77 152 397 909
Size: 77 75 245 512
Fragments arranged by size:
512 245 77 75
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 780 909
Size: 780 129
Enzymes that do cut:
AarI AcuI AlfI ApaI ApoI BanI BanII BbsI
BbvCI BclI BfrBI BglII Bme1580I BpmI Bpu10I BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI
BstYI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
MfeI MscI MslI NsiI PflMI PpuMI PspOMI SacI
SanDI SapI SfcI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BglI
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI