Restriction map for
pFN21AE5371
(Linear) (Six-base) MAPSORT of: KIEE5371.seq Check: 3572 from: 1 to: 897
KIEE5371.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
AccI GT'mk_AC
Cuts at: 0 233 897
Size: 233 664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 897
Size: 382 515
AgeI A'CCGG_T
Cuts at: 0 643 897
Size: 643 254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 653 897
Size: 653 244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 815 847 897
Size: 815 32 50
Fragments arranged by size:
815 50 32
AlwNI CAG_nnn'CTG
Cuts at: 0 287 897
Size: 287 610
ApoI r'AATT_y
Cuts at: 0 48 897
Size: 48 849
AseI AT'TA_AT
Cuts at: 0 572 897
Size: 572 325
AvaI C'yCGr_G
Cuts at: 0 276 897
Size: 276 621
BanII G_rGCy'C
Cuts at: 0 553 897
Size: 553 344
BlpI GC'TnA_GC
Cuts at: 0 448 897
Size: 448 449
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 572 897
Size: 572 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 803 897
Size: 544 259 94
Fragments arranged by size:
544 259 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 627 897
Size: 567 60 270
Fragments arranged by size:
567 270 60
BsaWI w'CCGG_w
Cuts at: 0 643 897
Size: 643 254
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 624 897
Size: 114 510 273
Fragments arranged by size:
510 273 114
BseYI C'CCAG_C
Cuts at: 0 174 897
Size: 174 723
BsiHKAI G_wGCw'C
Cuts at: 0 553 897
Size: 553 344
BsmBI CGTCTCn'nnnn_
Cuts at: 0 213 897
Size: 213 684
Bsp1286I G_dGCh'C
Cuts at: 0 553 897
Size: 553 344
BsrFI r'CCGG_y
Cuts at: 0 643 897
Size: 643 254
BsrGI T'GTAC_A
Cuts at: 0 437 897
Size: 437 460
BstYI r'GATC_y
Cuts at: 0 383 897
Size: 383 514
DraI TTT'AAA
Cuts at: 0 47 897
Size: 47 850
DraIII CAC_nnn'GTG
Cuts at: 0 348 897
Size: 348 549
EarI CTCTTCn'nnn_
Cuts at: 0 132 216 897
Size: 132 84 681
Fragments arranged by size:
681 132 84
EcoICRI GAG'CTC
Cuts at: 0 551 897
Size: 551 346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 544 803 897
Size: 382 162 259 94
Fragments arranged by size:
382 259 162 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 654 686 897
Size: 654 32 211
Fragments arranged by size:
654 211 32
MfeI C'AATT_G
Cuts at: 0 90 897
Size: 90 807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 897
Size: 363 534
MslI CAynn'nnrTG
Cuts at: 0 574 677 897
Size: 574 103 220
Fragments arranged by size:
574 220 103
PfoI T'CCnGG_A
Cuts at: 0 819 897
Size: 819 78
PspXI vC'TCGA_Gb
Cuts at: 0 276 897
Size: 276 621
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 184 216 897
Size: 184 32 681
Fragments arranged by size:
681 184 32
SacI G_AGCT'C
Cuts at: 0 553 897
Size: 553 344
SexAI A'CCwGG_T
Cuts at: 0 160 897
Size: 160 737
SmlI C'TyrA_G
Cuts at: 0 276 546 606 897
Size: 276 270 60 291
Fragments arranged by size:
291 276 270 60
TatI w'GTAC_w
Cuts at: 0 314 437 897
Size: 314 123 460
Fragments arranged by size:
460 314 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 897
Size: 396 501
XhoI C'TCGA_G
Cuts at: 0 276 897
Size: 276 621
Enzymes that do cut:
AccI AcuI AgeI AlfI AloI AlwNI ApoI AseI
AvaI BanII BlpI BplI BpmI BpuEI BsaWI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BsrFI BsrGI BstYI DraI
DraIII EarI EcoICRI Eco57MI Hin4I MfeI MmeI MslI
PfoI PspXI PsrI SacI SexAI SmlI TatI XcmI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BmtI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DrdI EaeI EagI EciI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XmaI XmnI ZraI