Restriction map for
pFN21AE5360
(Linear) (Six-base) MAPSORT of: KIEE5360.seq Check: 6905 from: 1 to: 780
KIEE5360.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
Acc65I G'GTAC_C
Cuts at: 0 614 780
Size: 614 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 655 780
Size: 421 234 125
Fragments arranged by size:
421 234 125
AlwNI CAG_nnn'CTG
Cuts at: 0 126 780
Size: 126 654
ApaI G_GGCC'C
Cuts at: 0 144 780
Size: 144 636
AvaI C'yCGr_G
Cuts at: 0 143 360 780
Size: 143 217 420
Fragments arranged by size:
420 217 143
BanI G'GyrC_C
Cuts at: 0 147 156 350 614 780
Size: 147 9 194 264 166
Fragments arranged by size:
264 194 166 147 9
BanII G_rGCy'C
Cuts at: 0 144 197 474 780
Size: 144 53 277 306
Fragments arranged by size:
306 277 144 53
BbeI G_GCGC'C
Cuts at: 0 151 780
Size: 151 629
BbsI GAAGACnn'nnnn_
Cuts at: 0 470 672 780
Size: 470 202 108
Fragments arranged by size:
470 202 108
BlpI GC'TnA_GC
Cuts at: 0 38 780
Size: 38 742
Bme1580I G_kGCm'C
Cuts at: 0 144 161 360 409 780
Size: 144 17 199 49 371
Fragments arranged by size:
371 199 144 49 17
BmgBI CAC'GTC
Cuts at: 0 543 780
Size: 543 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 192 780
Size: 192 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 780
Size: 75 705
Bpu10I CC'TnA_GC
Cuts at: 0 641 780
Size: 641 139
BsaHI Gr'CG_yC
Cuts at: 0 148 780
Size: 148 632
BsaWI w'CCGG_w
Cuts at: 0 299 722 780
Size: 299 423 58
Fragments arranged by size:
423 299 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 277 624 780
Size: 22 255 347 156
Fragments arranged by size:
347 255 156 22
BseYI C'CCAG_C
Cuts at: 0 230 250 619 780
Size: 230 20 369 161
Fragments arranged by size:
369 230 161 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 361 780
Size: 160 201 419
Fragments arranged by size:
419 201 160
BsiEI CG_ry'CG
Cuts at: 0 375 451 780
Size: 375 76 329
Fragments arranged by size:
375 329 76
BsiHKAI G_wGCw'C
Cuts at: 0 197 780
Size: 197 583
BsmBI CGTCTCn'nnnn_
Cuts at: 0 533 780
Size: 533 247
Bsp1286I G_dGCh'C
Cuts at: 0 144 161 197 360 409 474 780
Size: 144 17 36 163 49 65 306
Fragments arranged by size:
306 163 144 65 49 36 17
BspEI T'CCGG_A
Cuts at: 0 299 780
Size: 299 481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 780
Size: 182 598
BsrBI CCG'CTC
Cuts at: 0 138 453 780
Size: 138 315 327
Fragments arranged by size:
327 315 138
BsrFI r'CCGG_y
Cuts at: 0 374 395 780
Size: 374 21 385
Fragments arranged by size:
385 374 21
BssSI C'ACGA_G
Cuts at: 0 473 742 780
Size: 473 269 38
Fragments arranged by size:
473 269 38
BstXI CCAn_nnnn'nTGG
Cuts at: 0 258 780
Size: 258 522
BstYI r'GATC_y
Cuts at: 0 564 780
Size: 564 216
BtgI C'CryG_G
Cuts at: 0 414 430 780
Size: 414 16 350
Fragments arranged by size:
414 350 16
EaeI y'GGCC_r
Cuts at: 0 372 448 780
Size: 372 76 332
Fragments arranged by size:
372 332 76
EagI C'GGCC_G
Cuts at: 0 372 448 780
Size: 372 76 332
Fragments arranged by size:
372 332 76
EarI CTCTTCn'nnn_
Cuts at: 0 601 780
Size: 601 179
EcoICRI GAG'CTC
Cuts at: 0 195 780
Size: 195 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 421 655 780
Size: 75 346 234 125
Fragments arranged by size:
346 234 125 75
EcoO109I rG'GnC_Cy
Cuts at: 0 522 780
Size: 522 258
HaeII r_GCGC'y
Cuts at: 0 151 390 510 780
Size: 151 239 120 270
Fragments arranged by size:
270 239 151 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 30 42 437 469 556 588 780
Size: 10 20 12 395 32 87 32 192
Fragments arranged by size:
395 192 87 32 32 20 12 10
KasI G'GCGC_C
Cuts at: 0 147 780
Size: 147 633
KpnI G_GTAC'C
Cuts at: 0 618 780
Size: 618 162
MspA1I CmG'CkG
Cuts at: 0 42 483 492 587 694 780
Size: 42 441 9 95 107 86
Fragments arranged by size:
441 107 95 86 42 9
NaeI GCC'GGC
Cuts at: 0 376 780
Size: 376 404
NarI GG'CG_CC
Cuts at: 0 148 780
Size: 148 632
NcoI C'CATG_G
Cuts at: 0 414 780
Size: 414 366
NgoMIV G'CCGG_C
Cuts at: 0 374 780
Size: 374 406
PflMI CCAn_nnn'nTGG
Cuts at: 0 126 780
Size: 126 654
PfoI T'CCnGG_A
Cuts at: 0 53 780
Size: 53 727
PpuMI rG'GwC_Cy
Cuts at: 0 522 780
Size: 522 258
PspOMI G'GGCC_C
Cuts at: 0 140 780
Size: 140 640
PstI C_TGCA'G
Cuts at: 0 497 780
Size: 497 283
PvuII CAG'CTG
Cuts at: 0 42 492 694 780
Size: 42 450 202 86
Fragments arranged by size:
450 202 86 42
SacI G_AGCT'C
Cuts at: 0 197 780
Size: 197 583
SfcI C'TryA_G
Cuts at: 0 493 780
Size: 493 287
SfoI GGC'GCC
Cuts at: 0 149 780
Size: 149 631
SmaI CCC'GGG
Cuts at: 0 145 780
Size: 145 635
SrfI GCCC'GGGC
Cuts at: 0 145 780
Size: 145 635
StyI C'CwwG_G
Cuts at: 0 414 686 780
Size: 414 272 94
Fragments arranged by size:
414 272 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 780
Size: 723 57
XmaI C'CCGG_G
Cuts at: 0 143 780
Size: 143 637
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
KasI KpnI MspA1I NaeI NarI NcoI NgoMIV PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SfcI SfoI
SmaI SrfI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaXI BsiWI BsmI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MmeI MscI MslI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmnI ZraI