Restriction map for
pFN21AE5358
(Linear) (Six-base) MAPSORT of: KIEE5358.seq Check: 9400 from: 1 to: 921
KIEE5358.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
AccI GT'mk_AC
Cuts at: 0 815 921
Size: 815 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 718 837 921
Size: 385 333 119 84
Fragments arranged by size:
385 333 119 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 139 921
Size: 139 782
AlwNI CAG_nnn'CTG
Cuts at: 0 891 921
Size: 891 30
ApaLI G'TGCA_C
Cuts at: 0 190 921
Size: 190 731
ApoI r'AATT_y
Cuts at: 0 472 487 766 921
Size: 472 15 279 155
Fragments arranged by size:
472 279 155 15
AseI AT'TA_AT
Cuts at: 0 611 921
Size: 611 310
BanI G'GyrC_C
Cuts at: 0 154 387 921
Size: 154 233 534
Fragments arranged by size:
534 233 154
BanII G_rGCy'C
Cuts at: 0 409 696 921
Size: 409 287 225
Fragments arranged by size:
409 287 225
BbsI GAAGACnn'nnnn_
Cuts at: 0 43 921
Size: 43 878
BclI T'GATC_A
Cuts at: 0 749 921
Size: 749 172
Bme1580I G_kGCm'C
Cuts at: 0 194 392 656 921
Size: 194 198 264 265
Fragments arranged by size:
265 264 198 194
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 647 921
Size: 647 274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 921
Size: 847 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 241 597 921
Size: 241 356 324
Fragments arranged by size:
356 324 241
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 636 921
Size: 636 285
BseYI C'CCAG_C
Cuts at: 0 90 670 921
Size: 90 580 251
Fragments arranged by size:
580 251 90
BsiEI CG_ry'CG
Cuts at: 0 243 921
Size: 243 678
BsiHKAI G_wGCw'C
Cuts at: 0 194 696 921
Size: 194 502 225
Fragments arranged by size:
502 225 194
BsmI GAATG_Cn'
Cuts at: 0 644 921
Size: 644 277
Bsp1286I G_dGCh'C
Cuts at: 0 194 392 409 656 696 921
Size: 194 198 17 247 40 225
Fragments arranged by size:
247 225 198 194 40 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 921
Size: 107 814
BstAPI GCAn_nnn'nTGC
Cuts at: 0 660 921
Size: 660 261
BstBI TT'CG_AA
Cuts at: 0 870 921
Size: 870 51
BtgI C'CryG_G
Cuts at: 0 799 921
Size: 799 122
EaeI y'GGCC_r
Cuts at: 0 240 796 921
Size: 240 556 125
Fragments arranged by size:
556 240 125
EagI C'GGCC_G
Cuts at: 0 240 921
Size: 240 681
EcoICRI GAG'CTC
Cuts at: 0 694 921
Size: 694 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 718 837 921
Size: 385 333 119 84
Fragments arranged by size:
385 333 119 84
EcoO109I rG'GnC_Cy
Cuts at: 0 666 921
Size: 666 255
HindIII A'AGCT_T
Cuts at: 0 865 921
Size: 865 56
MscI TGG'CCA
Cuts at: 0 798 921
Size: 798 123
MspA1I CmG'CkG
Cuts at: 0 302 921
Size: 302 619
NcoI C'CATG_G
Cuts at: 0 799 921
Size: 799 122
PacI TTA_AT'TAA
Cuts at: 0 611 921
Size: 611 310
PasI CC'CwG_GG
Cuts at: 0 379 921
Size: 379 542
PflMI CCAn_nnn'nTGG
Cuts at: 0 290 921
Size: 290 631
PpuMI rG'GwC_Cy
Cuts at: 0 666 921
Size: 666 255
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 490 522 775 807 921
Size: 490 32 253 32 114
Fragments arranged by size:
490 253 114 32 32
PstI C_TGCA'G
Cuts at: 0 176 665 921
Size: 176 489 256
Fragments arranged by size:
489 256 176
PvuII CAG'CTG
Cuts at: 0 302 921
Size: 302 619
SacI G_AGCT'C
Cuts at: 0 696 921
Size: 696 225
SbfI CC_TGCA'GG
Cuts at: 0 665 921
Size: 665 256
ScaI AGT'ACT
Cuts at: 0 226 921
Size: 226 695
SfcI C'TryA_G
Cuts at: 0 172 661 921
Size: 172 489 260
Fragments arranged by size:
489 260 172
SmlI C'TyrA_G
Cuts at: 0 862 921
Size: 862 59
StyI C'CwwG_G
Cuts at: 0 128 799 921
Size: 128 671 122
Fragments arranged by size:
671 128 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 142 375 438 691 903 921
Size: 142 233 63 253 212 18
Fragments arranged by size:
253 233 212 142 63 18
TatI w'GTAC_w
Cuts at: 0 224 921
Size: 224 697
XmnI GAAnn'nnTTC
Cuts at: 0 73 489 921
Size: 73 416 432
Fragments arranged by size:
432 416 73
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI AseI BanI
BanII BbsI BclI Bme1580I BplI BpuEI BsaI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BstAPI BstBI
BtgI EaeI EagI EcoICRI Eco57MI EcoO109I HindIII MscI
MspA1I NcoI PacI PasI PflMI PpuMI PsrI PstI
PvuII SacI SbfI ScaI SfcI SmlI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacII SalI SanDI SapI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI