(Linear) (Six-base) MAPSORT of: KIEE5357.seq  Check: 2325  from: 1  to: 927



KIEE5357.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     876     927 
   Size:        876      51
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637     927 
   Size:        637     290
 
AflIII A'CryG_T

Cuts at:      0     443     927 
   Size:        443     484
 
AlwNI CAG_nnn'CTG

Cuts at:      0     287     927 
   Size:        287     640
 
ApoI r'AATT_y

Cuts at:      0      60      90     750     927 
   Size:         60      30     660     177

  Fragments arranged by size: 

                 660     177      60      30
 
BbvCI CC'TCA_GC

Cuts at:      0     126     304     927 
   Size:        126     178     623

  Fragments arranged by size: 

                 623     178     126
 
BclI T'GATC_A

Cuts at:      0     132     927 
   Size:        132     795
 
BglII A'GATC_T

Cuts at:      0     119     927 
   Size:        119     808
 
BmtI G_CTAG'C

Cuts at:      0     547     927 
   Size:        547     380
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     324     927 
   Size:        144     180     603

  Fragments arranged by size: 

                 603     180     144
 
Bpu10I CC'TnA_GC

Cuts at:      0     126     304     927 
   Size:        126     178     623

  Fragments arranged by size: 

                 623     178     126
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     632     927 
   Size:        307     325     295

  Fragments arranged by size: 

                 325     307     295
 
BsaAI yAC'GTr

Cuts at:      0     446     927 
   Size:        446     481
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     114     927 
   Size:        114     813
 
BseYI C'CCAG_C

Cuts at:      0     655     896     927 
   Size:        655     241      31

  Fragments arranged by size: 

                 655     241      31
 
BsmI GAATG_Cn'

Cuts at:      0     513     927 
   Size:        513     414
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     876     927 
   Size:        876      51
 
BsrDI GCAATG_nn'

Cuts at:      0      95     518     927 
   Size:         95     423     409

  Fragments arranged by size: 

                 423     409      95
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     799     927 
   Size:        799     128
 
BstYI r'GATC_y

Cuts at:      0     119     643     651     665     927 
   Size:        119     524       8      14     262

  Fragments arranged by size: 

                 524     262     119      14       8
 
BtgI C'CryG_G

Cuts at:      0     633     927 
   Size:        633     294
 
DraI TTT'AAA

Cuts at:      0     233     927 
   Size:        233     694
 
DraIII CAC_nnn'GTG

Cuts at:      0     432     927 
   Size:        432     495
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     324     637     927 
   Size:        144     180     313     290

  Fragments arranged by size: 

                 313     290     180     144
 
EcoRV GAT'ATC

Cuts at:      0     578     927 
   Size:        578     349
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     882     927 
   Size:        882      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     927 
   Size:        104      32     791

  Fragments arranged by size: 

                 791     104      32
 
HincII GTy'rAC

Cuts at:      0     920     927 
   Size:        920       7
 
HindIII A'AGCT_T

Cuts at:      0     794     927 
   Size:        794     133
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     548     927 
   Size:        241     307     379

  Fragments arranged by size: 

                 379     307     241
 
MspA1I CmG'CkG

Cuts at:      0     130     927 
   Size:        130     797
 
NcoI C'CATG_G

Cuts at:      0     633     927 
   Size:        633     294
 
NheI G'CTAG_C

Cuts at:      0     543     927 
   Size:        543     384
 
PvuII CAG'CTG

Cuts at:      0     130     927 
   Size:        130     797
 
SfcI C'TryA_G

Cuts at:      0     533     927 
   Size:        533     394
 
SmlI C'TyrA_G

Cuts at:      0     322     647     927 
   Size:        322     325     280

  Fragments arranged by size: 

                 325     322     280
 
SspI AAT'ATT

Cuts at:      0     185     927 
   Size:        185     742
 
StyI C'CwwG_G

Cuts at:      0     633     927 
   Size:        633     294
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     927 
   Size:         36     891
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApoI    BbvCI     BclI    BglII
     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BseRI    BseYI     BsmI
    BspMI    BsrDI    BstXI    BstYI     BtgI     DraI   DraIII  Eco57MI
    EcoRV     FalI    Hin4I   HincII  HindIII     MmeI   MspA1I     NcoI
     NheI    PvuII     SfcI     SmlI     SspI     StyI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI