Restriction map for
pFN21AE5356
(Linear) (Six-base) MAPSORT of: KIEE5356.seq Check: 8120 from: 1 to: 927
KIEE5356.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 29 927
Size: 29 898
AclI AA'CG_TT
Cuts at: 0 893 927
Size: 893 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 927
Size: 544 383
AfeI AGC'GCT
Cuts at: 0 100 927
Size: 100 827
AhdI GACnn_n'nnGTC
Cuts at: 0 321 927
Size: 321 606
AleI CACnn'nnGTG
Cuts at: 0 502 927
Size: 502 425
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 823 855 927
Size: 823 32 72
Fragments arranged by size:
823 72 32
AlwNI CAG_nnn'CTG
Cuts at: 0 21 927
Size: 21 906
ApoI r'AATT_y
Cuts at: 0 166 650 927
Size: 166 484 277
Fragments arranged by size:
484 277 166
BanI G'GyrC_C
Cuts at: 0 682 927
Size: 682 245
BanII G_rGCy'C
Cuts at: 0 152 482 927
Size: 152 330 445
Fragments arranged by size:
445 330 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 174 532 927
Size: 174 358 395
Fragments arranged by size:
395 358 174
BciVI GTATCCnnnnn_n'
Cuts at: 0 570 927
Size: 570 357
BclI T'GATC_A
Cuts at: 0 396 927
Size: 396 531
BfrBI ATG'CAT
Cuts at: 0 856 927
Size: 856 71
BglI GCCn_nnn'nGGC
Cuts at: 0 55 927
Size: 55 872
BglII A'GATC_T
Cuts at: 0 464 927
Size: 464 463
Bme1580I G_kGCm'C
Cuts at: 0 137 927
Size: 137 790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 850 919 927
Size: 565 285 69 8
Fragments arranged by size:
565 285 69 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 927
Size: 466 461
BsaWI w'CCGG_w
Cuts at: 0 425 927
Size: 425 502
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 823 853 927
Size: 34 30 759 30 74
Fragments arranged by size:
759 74 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 927
Size: 343 584
BseYI C'CCAG_C
Cuts at: 0 95 115 306 927
Size: 95 20 191 621
Fragments arranged by size:
621 191 95 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 927
Size: 491 436
BsiHKAI G_wGCw'C
Cuts at: 0 482 927
Size: 482 445
Bsp1286I G_dGCh'C
Cuts at: 0 137 152 482 927
Size: 137 15 330 445
Fragments arranged by size:
445 330 137 15
BspEI T'CCGG_A
Cuts at: 0 425 927
Size: 425 502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 29 611 927
Size: 29 582 316
Fragments arranged by size:
582 316 29
BsrGI T'GTAC_A
Cuts at: 0 41 927
Size: 41 886
BstAPI GCAn_nnn'nTGC
Cuts at: 0 21 927
Size: 21 906
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 927
Size: 231 696
BstYI r'GATC_y
Cuts at: 0 464 927
Size: 464 463
BtgI C'CryG_G
Cuts at: 0 230 927
Size: 230 697
EaeI y'GGCC_r
Cuts at: 0 238 927
Size: 238 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 927
Size: 94 833
EcoICRI GAG'CTC
Cuts at: 0 480 927
Size: 480 447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 565 850 919 927
Size: 544 21 285 69 8
Fragments arranged by size:
544 285 69 21 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 205 927
Size: 25 180 722
Fragments arranged by size:
722 180 25
EcoO109I rG'GnC_Cy
Cuts at: 0 489 519 927
Size: 489 30 408
Fragments arranged by size:
489 408 30
EcoRI G'AATT_C
Cuts at: 0 166 927
Size: 166 761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 51 927
Size: 51 876
HaeII r_GCGC'y
Cuts at: 0 102 927
Size: 102 825
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 719 751 863 895 927
Size: 307 32 380 32 112 32 32
Fragments arranged by size:
380 307 112 32 32 32 32
HincII GTy'rAC
Cuts at: 0 717 927
Size: 717 210
MslI CAynn'nnrTG
Cuts at: 0 79 229 502 763 927
Size: 79 150 273 261 164
Fragments arranged by size:
273 261 164 150 79
NsiI A_TGCA'T
Cuts at: 0 858 927
Size: 858 69
NspI r_CATG'y
Cuts at: 0 84 840 927
Size: 84 756 87
Fragments arranged by size:
756 87 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 385 927
Size: 385 542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 927
Size: 823 32 72
Fragments arranged by size:
823 72 32
PpuMI rG'GwC_Cy
Cuts at: 0 489 519 927
Size: 489 30 408
Fragments arranged by size:
489 408 30
PstI C_TGCA'G
Cuts at: 0 26 608 911 927
Size: 26 582 303 16
Fragments arranged by size:
582 303 26 16
SacI G_AGCT'C
Cuts at: 0 482 927
Size: 482 445
SbfI CC_TGCA'GG
Cuts at: 0 608 927
Size: 608 319
SfcI C'TryA_G
Cuts at: 0 22 604 907 927
Size: 22 582 303 20
Fragments arranged by size:
582 303 22 20
SmlI C'TyrA_G
Cuts at: 0 481 927
Size: 481 446
StuI AGG'CCT
Cuts at: 0 640 927
Size: 640 287
StyI C'CwwG_G
Cuts at: 0 53 927
Size: 53 874
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 927
Size: 298 629
TatI w'GTAC_w
Cuts at: 0 41 927
Size: 41 886
XmnI GAAnn'nnTTC
Cuts at: 0 31 433 652 927
Size: 31 402 219 275
Fragments arranged by size:
402 275 219 31
Enzymes that do cut:
AarI AclI AcuI AfeI AhdI AleI AloI AlwNI
ApoI BanI BanII BbsI BciVI BclI BfrBI BglI
BglII Bme1580I BpmI BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrGI BstAPI BstXI
BstYI BtgI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII MslI NsiI NspI
PflMI PpiI PpuMI PstI SacI SbfI SfcI SmlI
StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AflII AflIII AgeI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI