Restriction map for
pFN21AE5354
(Linear) (Six-base) MAPSORT of: KIEE5354.seq Check: 2224 from: 1 to: 798
KIEE5354.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 112 798
Size: 112 686
AccI GT'mk_AC
Cuts at: 0 236 798
Size: 236 562
AfeI AGC'GCT
Cuts at: 0 98 798
Size: 98 700
AflII C'TTAA_G
Cuts at: 0 79 798
Size: 79 719
ApoI r'AATT_y
Cuts at: 0 653 749 798
Size: 653 96 49
Fragments arranged by size:
653 96 49
AseI AT'TA_AT
Cuts at: 0 317 798
Size: 317 481
BanI G'GyrC_C
Cuts at: 0 85 798
Size: 85 713
BbeI G_GCGC'C
Cuts at: 0 89 798
Size: 89 709
BbsI GAAGACnn'nnnn_
Cuts at: 0 175 255 798
Size: 175 80 543
Fragments arranged by size:
543 175 80
BlpI GC'TnA_GC
Cuts at: 0 489 798
Size: 489 309
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 798
Size: 306 492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 798
Size: 296 502
Bpu10I CC'TnA_GC
Cuts at: 0 41 798
Size: 41 757
BsaAI yAC'GTr
Cuts at: 0 6 798
Size: 6 792
BsaBI GATnn'nnATC
Cuts at: 0 110 798
Size: 110 688
BsaHI Gr'CG_yC
Cuts at: 0 86 798
Size: 86 712
BsmI GAATG_Cn'
Cuts at: 0 540 798
Size: 540 258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 112 798
Size: 112 686
BsrFI r'CCGG_y
Cuts at: 0 31 444 798
Size: 31 413 354
Fragments arranged by size:
413 354 31
BsrGI T'GTAC_A
Cuts at: 0 126 798
Size: 126 672
BstEII G'GTnAC_C
Cuts at: 0 27 798
Size: 27 771
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 203 798
Size: 203 595
DraI TTT'AAA
Cuts at: 0 713 798
Size: 713 85
DrdI GACnn_nn'nnGTC
Cuts at: 0 262 798
Size: 262 536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 798
Size: 296 502
EcoRI G'AATT_C
Cuts at: 0 749 798
Size: 749 49
HaeII r_GCGC'y
Cuts at: 0 25 73 89 100 798
Size: 25 48 16 11 698
Fragments arranged by size:
698 48 25 16 11
HincII GTy'rAC
Cuts at: 0 558 798
Size: 558 240
HpaI GTT'AAC
Cuts at: 0 558 798
Size: 558 240
KasI G'GCGC_C
Cuts at: 0 85 798
Size: 85 713
MfeI C'AATT_G
Cuts at: 0 578 798
Size: 578 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 798
Size: 456 342
NarI GG'CG_CC
Cuts at: 0 86 798
Size: 86 712
PmlI CAC'GTG
Cuts at: 0 6 798
Size: 6 792
SfoI GGC'GCC
Cuts at: 0 87 798
Size: 87 711
SmlI C'TyrA_G
Cuts at: 0 79 798
Size: 79 719
StyI C'CwwG_G
Cuts at: 0 89 798
Size: 89 709
TatI w'GTAC_w
Cuts at: 0 126 798
Size: 126 672
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 96 798
Size: 96 702
Enzymes that do cut:
AarI AccI AfeI AflII ApoI AseI BanI BbeI
BbsI BlpI BmrI BpmI Bpu10I BsaAI BsaBI BsaHI
BsmI BspMI BsrFI BsrGI BstEII BtgZI DraI DrdI
Eco57MI EcoRI HaeII HincII HpaI KasI MfeI MmeI
NarI PmlI SfoI SmlI StyI TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbvCI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraIII EaeI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
Hin4I HindIII KpnI MluI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI XmnI ZraI