Restriction map for
pFN21AE5350
(Linear) (Six-base) MAPSORT of: KIEE5350.seq Check: 427 from: 1 to: 918
KIEE5350.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AatII G_ACGT'C
Cuts at: 0 713 918
Size: 713 205
AflIII A'CryG_T
Cuts at: 0 544 918
Size: 544 374
AhdI GACnn_n'nnGTC
Cuts at: 0 721 918
Size: 721 197
AleI CACnn'nnGTG
Cuts at: 0 238 918
Size: 238 680
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 918
Size: 398 32 488
Fragments arranged by size:
488 398 32
AlwNI CAG_nnn'CTG
Cuts at: 0 699 795 918
Size: 699 96 123
Fragments arranged by size:
699 123 96
AvaI C'yCGr_G
Cuts at: 0 173 619 918
Size: 173 446 299
Fragments arranged by size:
446 299 173
BanI G'GyrC_C
Cuts at: 0 267 298 883 918
Size: 267 31 585 35
Fragments arranged by size:
585 267 35 31
BanII G_rGCy'C
Cuts at: 0 71 688 918
Size: 71 617 230
Fragments arranged by size:
617 230 71
BclI T'GATC_A
Cuts at: 0 242 918
Size: 242 676
BlpI GC'TnA_GC
Cuts at: 0 65 918
Size: 65 853
Bme1580I G_kGCm'C
Cuts at: 0 301 888 918
Size: 301 587 30
Fragments arranged by size:
587 301 30
BmgBI CAC'GTC
Cuts at: 0 777 918
Size: 777 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 376 616 918
Size: 109 267 240 302
Fragments arranged by size:
302 267 240 109
Bpu10I CC'TnA_GC
Cuts at: 0 524 687 918
Size: 524 163 231
Fragments arranged by size:
524 231 163
BsaHI Gr'CG_yC
Cuts at: 0 710 918
Size: 710 208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 240 270 398 428 918
Size: 240 30 128 30 490
Fragments arranged by size:
490 240 128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 307 457 619 918
Size: 112 195 150 162 299
Fragments arranged by size:
299 195 162 150 112
BseYI C'CCAG_C
Cuts at: 0 738 918
Size: 738 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 512 799 808 918
Size: 261 251 287 9 110
Fragments arranged by size:
287 261 251 110 9
BsmI GAATG_Cn'
Cuts at: 0 562 918
Size: 562 356
Bsp1286I G_dGCh'C
Cuts at: 0 71 301 688 888 918
Size: 71 230 387 200 30
Fragments arranged by size:
387 230 200 71 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 417 829 918
Size: 417 412 89
Fragments arranged by size:
417 412 89
BsrFI r'CCGG_y
Cuts at: 0 763 918
Size: 763 155
BstAPI GCAn_nnn'nTGC
Cuts at: 0 786 918
Size: 786 132
BstEII G'GTnAC_C
Cuts at: 0 816 918
Size: 816 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 345 768 918
Size: 345 423 150
Fragments arranged by size:
423 345 150
BtgI C'CryG_G
Cuts at: 0 518 918
Size: 518 400
EaeI y'GGCC_r
Cuts at: 0 103 638 797 918
Size: 103 535 159 121
Fragments arranged by size:
535 159 121 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 376 616 918
Size: 109 267 240 302
Fragments arranged by size:
302 267 240 109
EcoO109I rG'GnC_Cy
Cuts at: 0 110 349 857 918
Size: 110 239 508 61
Fragments arranged by size:
508 239 110 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 170 202 707 739 918
Size: 170 32 505 32 179
Fragments arranged by size:
505 179 170 32 32
MscI TGG'CCA
Cuts at: 0 105 640 918
Size: 105 535 278
Fragments arranged by size:
535 278 105
MslI CAynn'nnrTG
Cuts at: 0 221 238 918
Size: 221 17 680
Fragments arranged by size:
680 221 17
MspA1I CmG'CkG
Cuts at: 0 32 636 918
Size: 32 604 282
Fragments arranged by size:
604 282 32
NcoI C'CATG_G
Cuts at: 0 518 918
Size: 518 400
NspI r_CATG'y
Cuts at: 0 220 548 736 918
Size: 220 328 188 182
Fragments arranged by size:
328 220 188 182
PasI CC'CwG_GG
Cuts at: 0 345 852 918
Size: 345 507 66
Fragments arranged by size:
507 345 66
PciI A'CATG_T
Cuts at: 0 544 918
Size: 544 374
PflMI CCAn_nnn'nTGG
Cuts at: 0 519 918
Size: 519 399
PfoI T'CCnGG_A
Cuts at: 0 74 918
Size: 74 844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 398 430 918
Size: 398 32 488
Fragments arranged by size:
488 398 32
PpuMI rG'GwC_Cy
Cuts at: 0 110 349 857 918
Size: 110 239 508 61
Fragments arranged by size:
508 239 110 61
PstI C_TGCA'G
Cuts at: 0 88 170 756 918
Size: 88 82 586 162
Fragments arranged by size:
586 162 88 82
PvuII CAG'CTG
Cuts at: 0 32 636 918
Size: 32 604 282
Fragments arranged by size:
604 282 32
SanDI GG'GwC_CC
Cuts at: 0 349 918
Size: 349 569
SfcI C'TryA_G
Cuts at: 0 84 166 752 918
Size: 84 82 586 166
Fragments arranged by size:
586 166 84 82
SmaI CCC'GGG
Cuts at: 0 175 918
Size: 175 743
SphI G_CATG'C
Cuts at: 0 220 918
Size: 220 698
StyI C'CwwG_G
Cuts at: 0 106 518 641 918
Size: 106 412 123 277
Fragments arranged by size:
412 277 123 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 240 272 918
Size: 240 32 646
Fragments arranged by size:
646 240 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 918
Size: 525 393
XmaI C'CCGG_G
Cuts at: 0 173 918
Size: 173 745
ZraI GAC'GTC
Cuts at: 0 711 918
Size: 711 207
Enzymes that do cut:
AatII AflIII AhdI AleI AloI AlwNI AvaI BanI
BanII BclI BlpI Bme1580I BmgBI BpmI Bpu10I BsaHI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrFI
BstAPI BstEII BstXI BtgI EaeI Eco57MI EcoO109I Hin4I
MscI MslI MspA1I NcoI NspI PasI PciI PflMI
PfoI PpiI PpuMI PstI PvuII SanDI SfcI SmaI
SphI StyI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XhoI XmnI