Restriction map for
pFN21AE5347
(Linear) (Six-base) MAPSORT of: KIEE5347.seq Check: 3398 from: 1 to: 864
KIEE5347.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AccI GT'mk_AC
Cuts at: 0 284 864
Size: 284 580
Acc65I G'GTAC_C
Cuts at: 0 131 864
Size: 131 733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 864
Size: 787 77
AhdI GACnn_n'nnGTC
Cuts at: 0 592 864
Size: 592 272
AleI CACnn'nnGTG
Cuts at: 0 640 864
Size: 640 224
AlwNI CAG_nnn'CTG
Cuts at: 0 333 864
Size: 333 531
AvaI C'yCGr_G
Cuts at: 0 495 864
Size: 495 369
BanI G'GyrC_C
Cuts at: 0 131 864
Size: 131 733
BanII G_rGCy'C
Cuts at: 0 167 864
Size: 167 697
BbsI GAAGACnn'nnnn_
Cuts at: 0 9 864
Size: 9 855
BciVI GTATCCnnnnn_n'
Cuts at: 0 838 864
Size: 838 26
Bme1580I G_kGCm'C
Cuts at: 0 576 699 864
Size: 576 123 165
Fragments arranged by size:
576 165 123
BmgBI CAC'GTC
Cuts at: 0 33 864
Size: 33 831
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 347 864
Size: 347 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 457 684 864
Size: 423 34 227 180
Fragments arranged by size:
423 227 180 34
BsaAI yAC'GTr
Cuts at: 0 273 627 864
Size: 273 354 237
Fragments arranged by size:
354 273 237
BsaHI Gr'CG_yC
Cuts at: 0 363 864
Size: 363 501
BsaWI w'CCGG_w
Cuts at: 0 128 864
Size: 128 736
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 838 844 864
Size: 430 30 378 6 20
Fragments arranged by size:
430 378 30 20 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 787 790 846 849 864
Size: 787 3 56 3 15
Fragments arranged by size:
787 56 15 3 3
BseYI C'CCAG_C
Cuts at: 0 172 294 793 864
Size: 172 122 499 71
Fragments arranged by size:
499 172 122 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 468 568 864
Size: 451 17 100 296
Fragments arranged by size:
451 296 100 17
BsiEI CG_ry'CG
Cuts at: 0 212 864
Size: 212 652
Bsp1286I G_dGCh'C
Cuts at: 0 167 576 699 864
Size: 167 409 123 165
Fragments arranged by size:
409 167 165 123
BsrFI r'CCGG_y
Cuts at: 0 239 864
Size: 239 625
BsrGI T'GTAC_A
Cuts at: 0 230 864
Size: 230 634
BstEII G'GTnAC_C
Cuts at: 0 177 864
Size: 177 687
BstZ17I GTA'TAC
Cuts at: 0 285 864
Size: 285 579
BtgI C'CryG_G
Cuts at: 0 159 864
Size: 159 705
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 683 864
Size: 683 181
EaeI y'GGCC_r
Cuts at: 0 188 209 344 864
Size: 188 21 135 520
Fragments arranged by size:
520 188 135 21
EagI C'GGCC_G
Cuts at: 0 209 864
Size: 209 655
EarI CTCTTCn'nnn_
Cuts at: 0 510 864
Size: 510 354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 457 684 787 864
Size: 423 34 227 103 77
Fragments arranged by size:
423 227 103 77 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 688 820 864
Size: 688 132 44
Fragments arranged by size:
688 132 44
EcoO109I rG'GnC_Cy
Cuts at: 0 299 864
Size: 299 565
HaeII r_GCGC'y
Cuts at: 0 541 864
Size: 541 323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 348 380 578 610 864
Size: 348 32 198 32 254
Fragments arranged by size:
348 254 198 32 32
HincII GTy'rAC
Cuts at: 0 414 812 864
Size: 414 398 52
Fragments arranged by size:
414 398 52
KpnI G_GTAC'C
Cuts at: 0 135 864
Size: 135 729
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 864
Size: 544 320
MscI TGG'CCA
Cuts at: 0 346 864
Size: 346 518
MslI CAynn'nnrTG
Cuts at: 0 640 864
Size: 640 224
MspA1I CmG'CkG
Cuts at: 0 6 435 864
Size: 6 429 429
Fragments arranged by size:
429 429 6
PasI CC'CwG_GG
Cuts at: 0 816 864
Size: 816 48
PpuMI rG'GwC_Cy
Cuts at: 0 299 864
Size: 299 565
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 76 108 864
Size: 76 32 756
Fragments arranged by size:
756 76 32
PstI C_TGCA'G
Cuts at: 0 692 864
Size: 692 172
PvuII CAG'CTG
Cuts at: 0 435 864
Size: 435 429
SbfI CC_TGCA'GG
Cuts at: 0 692 864
Size: 692 172
SexAI A'CCwGG_T
Cuts at: 0 224 864
Size: 224 640
SfcI C'TryA_G
Cuts at: 0 688 864
Size: 688 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 411 864
Size: 172 239 453
Fragments arranged by size:
453 239 172
TatI w'GTAC_w
Cuts at: 0 230 350 518 864
Size: 230 120 168 346
Fragments arranged by size:
346 230 168 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 844 864
Size: 430 32 382 20
Fragments arranged by size:
430 382 32 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 838 864
Size: 838 26
Enzymes that do cut:
AccI Acc65I AcuI AhdI AleI AlwNI AvaI BanI
BanII BbsI BciVI Bme1580I BmgBI BplI BpmI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I
BsrFI BsrGI BstEII BstZ17I BtgI BtgZI EaeI EagI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI
MmeI MscI MslI MspA1I PasI PpuMI PsrI PstI
PvuII SbfI SexAI SfcI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI BmrI BmtI Bpu10I BpuEI BsaI BsaBI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI