(Linear) (Six-base) MAPSORT of: KIEE5342.seq  Check: 5520  from: 1  to: 819



KIEE5342.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:56  ..

AccI GT'mk_AC

Cuts at:      0     679     819 
   Size:        679     140
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380     819 
   Size:        380     439
 
AflIII A'CryG_T

Cuts at:      0     501     819 
   Size:        501     318
 
ApoI r'AATT_y

Cuts at:      0     658     708     819 
   Size:        658      50     111

  Fragments arranged by size: 

                 658     111      50
 
AvaI C'yCGr_G

Cuts at:      0     690     819 
   Size:        690     129
 
BamHI G'GATC_C

Cuts at:      0     632     819 
   Size:        632     187
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      35     819 
   Size:         35     784
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     223     819 
   Size:        223     596
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     819 
   Size:        493     326
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     658     819 
   Size:        658     161
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     469     819 
   Size:        469     350
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       9     143     819 
   Size:          9     134     676

  Fragments arranged by size: 

                 676     134       9
 
BseYI C'CCAG_C

Cuts at:      0     184     819 
   Size:        184     635
 
BsmI GAATG_Cn'

Cuts at:      0     123     798     819 
   Size:        123     675      21

  Fragments arranged by size: 

                 675     123      21
 
BspHI T'CATG_A

Cuts at:      0     453     819 
   Size:        453     366
 
BstBI TT'CG_AA

Cuts at:      0     705     819 
   Size:        705     114
 
BstYI r'GATC_y

Cuts at:      0     224     632     819 
   Size:        224     408     187

  Fragments arranged by size: 

                 408     224     187
 
BtgI C'CryG_G

Cuts at:      0     250     722     819 
   Size:        250     472      97

  Fragments arranged by size: 

                 472     250      97
 
EaeI y'GGCC_r

Cuts at:      0     247     819 
   Size:        247     572
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380     493     819 
   Size:        380     113     326

  Fragments arranged by size: 

                 380     326     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     819 
   Size:         14     805
 
EcoRI G'AATT_C

Cuts at:      0     658     819 
   Size:        658     161
 
HincII GTy'rAC

Cuts at:      0     509     819 
   Size:        509     310
 
HindIII A'AGCT_T

Cuts at:      0     319     819 
   Size:        319     500
 
MluI A'CGCG_T

Cuts at:      0     501     819 
   Size:        501     318
 
MslI CAynn'nnrTG

Cuts at:      0     814     819 
   Size:        814       5
 
MspA1I CmG'CkG

Cuts at:      0     112     819 
   Size:        112     707
 
NcoI C'CATG_G

Cuts at:      0     722     819 
   Size:        722      97
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     604     819 
   Size:        604     215
 
PfoI T'CCnGG_A

Cuts at:      0      49     819 
   Size:         49     770
 
PshAI GACnn'nnGTC

Cuts at:      0     308     819 
   Size:        308     511
 
PvuII CAG'CTG

Cuts at:      0     112     819 
   Size:        112     707
 
SfcI C'TryA_G

Cuts at:      0     653     819 
   Size:        653     166
 
SmlI C'TyrA_G

Cuts at:      0     637     819 
   Size:        637     182
 
SspI AAT'ATT

Cuts at:      0     751     819 
   Size:        751      68
 
StyI C'CwwG_G

Cuts at:      0     488     722     819 
   Size:        488     234      97

  Fragments arranged by size: 

                 488     234      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     181     198     665     819 
   Size:        181      17     467     154

  Fragments arranged by size: 

                 467     181     154      17
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI     AvaI    BamHI    BciVI     BmrI
     BpmI    BpuEI     BsaI    BseRI    BseYI     BsmI    BspHI    BstBI
    BstYI     BtgI     EaeI  Eco57MI EcoO109I    EcoRI   HincII  HindIII
     MluI     MslI   MspA1I     NcoI    PflMI     PfoI    PshAI    PvuII
     SfcI     SmlI     SspI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI
     KpnI     MfeI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI