Restriction map for
pFN21AE5340
(Linear) (Six-base) MAPSORT of: KIEE5340.seq Check: 6335 from: 1 to: 789
KIEE5340.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 767 789
Size: 46 721 22
Fragments arranged by size:
721 46 22
AflII C'TTAA_G
Cuts at: 0 154 789
Size: 154 635
AgeI A'CCGG_T
Cuts at: 0 70 97 789
Size: 70 27 692
Fragments arranged by size:
692 70 27
ApoI r'AATT_y
Cuts at: 0 291 789
Size: 291 498
AvaI C'yCGr_G
Cuts at: 0 716 789
Size: 716 73
AvrII C'CTAG_G
Cuts at: 0 564 789
Size: 564 225
BanI G'GyrC_C
Cuts at: 0 599 789
Size: 599 190
BciVI GTATCCnnnnn_n'
Cuts at: 0 739 789
Size: 739 50
BclI T'GATC_A
Cuts at: 0 581 789
Size: 581 208
BglII A'GATC_T
Cuts at: 0 383 789
Size: 383 406
Bme1580I G_kGCm'C
Cuts at: 0 397 789
Size: 397 392
BmgBI CAC'GTC
Cuts at: 0 618 789
Size: 618 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 789
Size: 139 650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 789
Size: 572 217
BsaAI yAC'GTr
Cuts at: 0 627 789
Size: 627 162
BsaWI w'CCGG_w
Cuts at: 0 70 97 789
Size: 70 27 692
Fragments arranged by size:
692 70 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 164 194 343 373 789
Size: 164 30 149 30 416
Fragments arranged by size:
416 164 149 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 74 364 789
Size: 74 290 425
Fragments arranged by size:
425 290 74
Bsp1286I G_dGCh'C
Cuts at: 0 397 789
Size: 397 392
BspHI T'CATG_A
Cuts at: 0 423 789
Size: 423 366
BsrDI GCAATG_nn'
Cuts at: 0 740 789
Size: 740 49
BsrFI r'CCGG_y
Cuts at: 0 70 97 789
Size: 70 27 692
Fragments arranged by size:
692 70 27
BsrGI T'GTAC_A
Cuts at: 0 330 789
Size: 330 459
BssSI C'ACGA_G
Cuts at: 0 5 789
Size: 5 784
BstAPI GCAn_nnn'nTGC
Cuts at: 0 54 789
Size: 54 735
BstEII G'GTnAC_C
Cuts at: 0 532 789
Size: 532 257
BstXI CCAn_nnnn'nTGG
Cuts at: 0 50 789
Size: 50 739
BstYI r'GATC_y
Cuts at: 0 383 607 789
Size: 383 224 182
Fragments arranged by size:
383 224 182
Bsu36I CC'TnA_GG
Cuts at: 0 142 347 789
Size: 142 205 442
Fragments arranged by size:
442 205 142
BtgI C'CryG_G
Cuts at: 0 700 789
Size: 700 89
BtsI GCAGTG_nn'
Cuts at: 0 789
Size: 789
ClaI AT'CG_AT
Cuts at: 0 215 789
Size: 215 574
EarI CTCTTCn'nnn_
Cuts at: 0 716 743 789
Size: 716 27 46
Fragments arranged by size:
716 46 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 721 789
Size: 721 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 572 767 789
Size: 46 526 195 22
Fragments arranged by size:
526 195 46 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 460 789
Size: 460 329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 339 789
Size: 339 450
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 789
Size: 105 32 652
Fragments arranged by size:
652 105 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 789
Size: 692 97
MslI CAynn'nnrTG
Cuts at: 0 463 789
Size: 463 326
MspA1I CmG'CkG
Cuts at: 0 201 789
Size: 201 588
NcoI C'CATG_G
Cuts at: 0 700 789
Size: 700 89
NspI r_CATG'y
Cuts at: 0 198 789
Size: 198 591
PmlI CAC'GTG
Cuts at: 0 627 789
Size: 627 162
SmlI C'TyrA_G
Cuts at: 0 154 789
Size: 154 635
SphI G_CATG'C
Cuts at: 0 198 789
Size: 198 591
StyI C'CwwG_G
Cuts at: 0 314 564 700 789
Size: 314 250 136 89
Fragments arranged by size:
314 250 136 89
TatI w'GTAC_w
Cuts at: 0 330 359 789
Size: 330 29 430
Fragments arranged by size:
430 330 29
Enzymes that do cut:
AcuI AflII AgeI ApoI AvaI AvrII BanI BciVI
BclI BglII Bme1580I BmgBI BplI BpmI BsaAI BsaWI
BsaXI BseRI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI
EarI EciI Eco57MI EcoNI FalI Hin4I MmeI MslI
MspA1I NcoI NspI PmlI SmlI SphI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI BmrI BmtI Bpu10I BpuEI BsaI
BsaBI BsaHI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BssHII BstBI BstZ17I BtgZI
CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI