Restriction map for
pFN21AE5337
(Linear) (Six-base) MAPSORT of: KIEE5337.seq Check: 3391 from: 1 to: 777
KIEE5337.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 377 777
Size: 377 400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 557 602 647 692 777
Size: 512 45 45 45 45 85
Fragments arranged by size:
512 85 45 45 45 45
AlwNI CAG_nnn'CTG
Cuts at: 0 490 535 580 625 670 777
Size: 490 45 45 45 45 107
Fragments arranged by size:
490 107 45 45 45 45
ApaLI G'TGCA_C
Cuts at: 0 204 777
Size: 204 573
ApoI r'AATT_y
Cuts at: 0 454 777
Size: 454 323
AvaI C'yCGr_G
Cuts at: 0 513 603 648 693 777
Size: 513 90 45 45 84
Fragments arranged by size:
513 90 84 45 45
BamHI G'GATC_C
Cuts at: 0 8 777
Size: 8 769
BanI G'GyrC_C
Cuts at: 0 340 389 460 777
Size: 340 49 71 317
Fragments arranged by size:
340 317 71 49
Bme1580I G_kGCm'C
Cuts at: 0 208 394 777
Size: 208 186 383
Fragments arranged by size:
383 208 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 777
Size: 209 568
BsaWI w'CCGG_w
Cuts at: 0 347 777
Size: 347 430
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 777
Size: 166 611
BseYI C'CCAG_C
Cuts at: 0 104 483 528 573 618 663 777
Size: 104 379 45 45 45 45 114
Fragments arranged by size:
379 114 104 45 45 45 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 777
Size: 285 492
BsiHKAI G_wGCw'C
Cuts at: 0 208 777
Size: 208 569
Bsp1286I G_dGCh'C
Cuts at: 0 208 394 777
Size: 208 186 383
Fragments arranged by size:
383 208 186
BspEI T'CCGG_A
Cuts at: 0 347 777
Size: 347 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 377 777
Size: 377 400
BsrGI T'GTAC_A
Cuts at: 0 262 777
Size: 262 515
BstYI r'GATC_y
Cuts at: 0 8 166 777
Size: 8 158 611
Fragments arranged by size:
611 158 8
BtsI GCAGTG_nn'
Cuts at: 0 80 777
Size: 80 697
DraIII CAC_nnn'GTG
Cuts at: 0 323 777
Size: 323 454
EarI CTCTTCn'nnn_
Cuts at: 0 488 533 578 623 668 714 777
Size: 488 45 45 45 45 46 63
Fragments arranged by size:
488 63 46 45 45 45 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 512 557 602 647 692 777
Size: 209 303 45 45 45 45 85
Fragments arranged by size:
303 209 85 45 45 45 45
EcoRV GAT'ATC
Cuts at: 0 438 777
Size: 438 339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 777
Size: 260 517
MspA1I CmG'CkG
Cuts at: 0 487 532 577 622 667 777
Size: 487 45 45 45 45 110
Fragments arranged by size:
487 110 45 45 45 45
PasI CC'CwG_GG
Cuts at: 0 559 777
Size: 559 218
PvuII CAG'CTG
Cuts at: 0 487 532 577 622 667 777
Size: 487 45 45 45 45 110
Fragments arranged by size:
487 110 45 45 45 45
SmaI CCC'GGG
Cuts at: 0 515 605 650 695 777
Size: 515 90 45 45 82
Fragments arranged by size:
515 90 82 45 45
TatI w'GTAC_w
Cuts at: 0 262 777
Size: 262 515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 777
Size: 471 306
XmaI C'CCGG_G
Cuts at: 0 513 603 648 693 777
Size: 513 90 45 45 84
Fragments arranged by size:
513 90 84 45 45
Enzymes that do cut:
AarI AcuI AlwNI ApaLI ApoI AvaI BamHI BanI
Bme1580I BpmI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BsrGI BstYI BtsI DraIII EarI Eco57MI
EcoRV MmeI MspA1I PasI PvuII SmaI TatI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XhoI XmnI ZraI