(Linear) (Six-base) MAPSORT of: KIEE5331.seq  Check: 7037  from: 1  to: 927



KIEE5331.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     897     927 
   Size:        897      30
 
AleI CACnn'nnGTG

Cuts at:      0     809     927 
   Size:        809     118
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     416     448     927 
   Size:        416      32     479

  Fragments arranged by size: 

                 479     416      32
 
ApoI r'AATT_y

Cuts at:      0      31     462     927 
   Size:         31     431     465

  Fragments arranged by size: 

                 465     431      31
 
AvaI C'yCGr_G

Cuts at:      0     118     927 
   Size:        118     809
 
AvrII C'CTAG_G

Cuts at:      0     765     927 
   Size:        765     162
 
BamHI G'GATC_C

Cuts at:      0     638     927 
   Size:        638     289
 
BanII G_rGCy'C

Cuts at:      0     900     927 
   Size:        900      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     692     927 
   Size:        692     235
 
BsaAI yAC'GTr

Cuts at:      0     676     927 
   Size:        676     251
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     489     927 
   Size:        373     116     438

  Fragments arranged by size: 

                 438     373     116
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     927 
   Size:        119     808
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     900     927 
   Size:        119     781      27

  Fragments arranged by size: 

                 781     119      27
 
BspHI T'CATG_A

Cuts at:      0     404     927 
   Size:        404     523
 
BsrGI T'GTAC_A

Cuts at:      0     830     927 
   Size:        830      97
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     105     927 
   Size:        105     822
 
BstYI r'GATC_y

Cuts at:      0     638     927 
   Size:        638     289
 
EaeI y'GGCC_r

Cuts at:      0     140     371     839     927 
   Size:        140     231     468      88

  Fragments arranged by size: 

                 468     231     140      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     897     927 
   Size:        897      30
 
EcoRI G'AATT_C

Cuts at:      0     462     927 
   Size:        462     465
 
HindIII A'AGCT_T

Cuts at:      0     477     927 
   Size:        477     450
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     927 
   Size:        215     712
 
MscI TGG'CCA

Cuts at:      0     142     373     841     927 
   Size:        142     231     468      86

  Fragments arranged by size: 

                 468     231     142      86
 
MslI CAynn'nnrTG

Cuts at:      0     172     298     592     809     847     927 
   Size:        172     126     294     217      38      80

  Fragments arranged by size: 

                 294     217     172     126      80      38
 
PfoI T'CCnGG_A

Cuts at:      0     442     927 
   Size:        442     485
 
SexAI A'CCwGG_T

Cuts at:      0     104     927 
   Size:        104     823
 
SfcI C'TryA_G

Cuts at:      0     867     927 
   Size:        867      60
 
StyI C'CwwG_G

Cuts at:      0     765     927 
   Size:        765     162
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     310     927 
   Size:        310     617
 
TatI w'GTAC_w

Cuts at:      0     176     312     701     830     927 
   Size:        176     136     389     129      97

  Fragments arranged by size: 

                 389     176     136     129      97
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     927 
   Size:         35     892
 

 Enzymes that do cut:

     AcuI     AleI     AloI     ApoI     AvaI    AvrII    BamHI    BanII
   Bpu10I    BsaAI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrGI    BstXI
    BstYI     EaeI  Eco57MI    EcoRI  HindIII     MmeI     MscI     MslI
     PfoI    SexAI     SfcI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI