Restriction map for
pFN21AE5329
(Linear) (Six-base) MAPSORT of: KIEE5329.seq Check: 4763 from: 1 to: 819
KIEE5329.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 433 819
Size: 433 386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 552 819
Size: 530 22 267
Fragments arranged by size:
530 267 22
AvrII C'CTAG_G
Cuts at: 0 243 819
Size: 243 576
BanII G_rGCy'C
Cuts at: 0 422 819
Size: 422 397
BciVI GTATCCnnnnn_n'
Cuts at: 0 468 819
Size: 468 351
BfrBI ATG'CAT
Cuts at: 0 305 819
Size: 305 514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 483 819
Size: 247 236 336
Fragments arranged by size:
336 247 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 819
Size: 254 565
BsaAI yAC'GTr
Cuts at: 0 594 819
Size: 594 225
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 819
Size: 244 575
BsiHKAI G_wGCw'C
Cuts at: 0 422 819
Size: 422 397
BsmI GAATG_Cn'
Cuts at: 0 127 819
Size: 127 692
Bsp1286I G_dGCh'C
Cuts at: 0 422 819
Size: 422 397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 433 819
Size: 433 386
BsrDI GCAATG_nn'
Cuts at: 0 114 692 819
Size: 114 578 127
Fragments arranged by size:
578 127 114
BsrGI T'GTAC_A
Cuts at: 0 86 819
Size: 86 733
BstEII G'GTnAC_C
Cuts at: 0 525 819
Size: 525 294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 819
Size: 225 594
Bsu36I CC'TnA_GG
Cuts at: 0 77 819
Size: 77 742
DraIII CAC_nnn'GTG
Cuts at: 0 208 819
Size: 208 611
EaeI y'GGCC_r
Cuts at: 0 463 611 819
Size: 463 148 208
Fragments arranged by size:
463 208 148
EcoICRI GAG'CTC
Cuts at: 0 420 819
Size: 420 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 483 530 552 819
Size: 247 236 47 22 267
Fragments arranged by size:
267 247 236 47 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 520 819
Size: 247 273 299
Fragments arranged by size:
299 273 247
EcoO109I rG'GnC_Cy
Cuts at: 0 503 647 819
Size: 503 144 172
Fragments arranged by size:
503 172 144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 236 819
Size: 236 583
HindIII A'AGCT_T
Cuts at: 0 359 819
Size: 359 460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 819
Size: 679 140
MscI TGG'CCA
Cuts at: 0 465 613 819
Size: 465 148 206
Fragments arranged by size:
465 206 148
MspA1I CmG'CkG
Cuts at: 0 237 572 819
Size: 237 335 247
Fragments arranged by size:
335 247 237
NsiI A_TGCA'T
Cuts at: 0 307 819
Size: 307 512
PflMI CCAn_nnn'nTGG
Cuts at: 0 556 819
Size: 556 263
PfoI T'CCnGG_A
Cuts at: 0 499 819
Size: 499 320
PmlI CAC'GTG
Cuts at: 0 594 819
Size: 594 225
PpuMI rG'GwC_Cy
Cuts at: 0 503 647 819
Size: 503 144 172
Fragments arranged by size:
503 172 144
PvuII CAG'CTG
Cuts at: 0 237 572 819
Size: 237 335 247
Fragments arranged by size:
335 247 237
SacI G_AGCT'C
Cuts at: 0 422 819
Size: 422 397
SexAI A'CCwGG_T
Cuts at: 0 521 819
Size: 521 298
SfcI C'TryA_G
Cuts at: 0 366 434 819
Size: 366 68 385
Fragments arranged by size:
385 366 68
SmlI C'TyrA_G
Cuts at: 0 269 819
Size: 269 550
StuI AGG'CCT
Cuts at: 0 253 515 819
Size: 253 262 304
Fragments arranged by size:
304 262 253
StyI C'CwwG_G
Cuts at: 0 153 243 819
Size: 153 90 576
Fragments arranged by size:
576 153 90
TatI w'GTAC_w
Cuts at: 0 86 819
Size: 86 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 131 163 819
Size: 131 32 656
Fragments arranged by size:
656 131 32
Enzymes that do cut:
AarI AcuI AvrII BanII BciVI BfrBI BpmI BpuEI
BsaAI BseRI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI
BstEII BstXI Bsu36I DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I FalI HindIII MmeI MscI MspA1I NsiI PflMI
PfoI PmlI PpuMI PvuII SacI SexAI SfcI SmlI
StuI StyI TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EagI EarI EciI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI