(Linear) (Six-base) MAPSORT of: KIEE5326.seq  Check: 5605  from: 1  to: 834



KIEE5326.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:55  ..

AccI GT'mk_AC

Cuts at:      0     596     834 
   Size:        596     238
 
AclI AA'CG_TT

Cuts at:      0     230     834 
   Size:        230     604
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     834 
   Size:        194     640
 
AflIII A'CryG_T

Cuts at:      0     599     834 
   Size:        599     235
 
AlwNI CAG_nnn'CTG

Cuts at:      0     373     426     834 
   Size:        373      53     408

  Fragments arranged by size: 

                 408     373      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     182     482     834 
   Size:        182     300     352

  Fragments arranged by size: 

                 352     300     182
 
BbvCI CC'TCA_GC

Cuts at:      0     427     834 
   Size:        427     407
 
BmgBI CAC'GTC

Cuts at:      0     600     834 
   Size:        600     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     625     834 
   Size:        394     231     209

  Fragments arranged by size: 

                 394     231     209
 
Bpu10I CC'TnA_GC

Cuts at:      0     427     834 
   Size:        427     407
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746     834 
   Size:        746      88
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     329     760     834 
   Size:        329     431      74

  Fragments arranged by size: 

                 431     329      74
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     634     754     834 
   Size:        634     120      80

  Fragments arranged by size: 

                 634     120      80
 
BspHI T'CATG_A

Cuts at:      0     389     834 
   Size:        389     445
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     347     381     834 
   Size:        347      34     453

  Fragments arranged by size: 

                 453     347      34
 
BsrBI CCG'CTC

Cuts at:      0     417     834 
   Size:        417     417
 
BsrDI GCAATG_nn'

Cuts at:      0     288     834 
   Size:        288     546
 
BstYI r'GATC_y

Cuts at:      0     404     834 
   Size:        404     430
 
BtgI C'CryG_G

Cuts at:      0     798     834 
   Size:        798      36
 
BtsI GCAGTG_nn'

Cuts at:      0     547     834 
   Size:        547     287
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     394     625     834 
   Size:        194     200     231     209

  Fragments arranged by size: 

                 231     209     200     194
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     472     834 
   Size:        472     362
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     800     834 
   Size:        800      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     389     421     615     647     799     831     834 
   Size:        389      32     194      32     152      32       3

  Fragments arranged by size: 

                 389     194     152      32      32      32       3
 
HincII GTy'rAC

Cuts at:      0      26     834 
   Size:         26     808
 
HindIII A'AGCT_T

Cuts at:      0     701     834 
   Size:        701     133
 
HpaI GTT'AAC

Cuts at:      0      26     834 
   Size:         26     808
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     834 
   Size:        213     621
 
MslI CAynn'nnrTG

Cuts at:      0     803     834 
   Size:        803      31
 
MspA1I CmG'CkG

Cuts at:      0     172     834 
   Size:        172     662
 
NcoI C'CATG_G

Cuts at:      0     798     834 
   Size:        798      36
 
NspI r_CATG'y

Cuts at:      0     300     834 
   Size:        300     534
 
PfoI T'CCnGG_A

Cuts at:      0     410     834 
   Size:        410     424
 
PstI C_TGCA'G

Cuts at:      0     378     834 
   Size:        378     456
 
SfcI C'TryA_G

Cuts at:      0     374     834 
   Size:        374     460
 
SmlI C'TyrA_G

Cuts at:      0     761     834 
   Size:        761      73
 
SspI AAT'ATT

Cuts at:      0     498     834 
   Size:        498     336
 
StyI C'CwwG_G

Cuts at:      0     798     834 
   Size:        798      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     196     834 
   Size:        196     638
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     649     834 
   Size:        649     185
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII    AlwNI     BbsI    BbvCI    BmgBI
     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BspHI    BspMI    BsrBI
    BsrDI    BstYI     BtgI     BtsI  Eco57MI    EcoNI     FalI    Hin4I
   HincII  HindIII     HpaI     MmeI     MslI   MspA1I     NcoI     NspI
     PfoI     PstI     SfcI     SmlI     SspI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI