(Linear) (Six-base) MAPSORT of: KIEE5325.seq  Check: 6051  from: 1  to: 909



KIEE5325.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     909 
   Size:        291     618
 
ApoI r'AATT_y

Cuts at:      0      46      60     495     538     909 
   Size:         46      14     435      43     371

  Fragments arranged by size: 

                 435     371      46      43      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     408     804     909 
   Size:        408     396     105

  Fragments arranged by size: 

                 408     396     105
 
BclI T'GATC_A

Cuts at:      0      86     909 
   Size:         86     823
 
BfrBI ATG'CAT

Cuts at:      0     450     909 
   Size:        450     459
 
Bme1580I G_kGCm'C

Cuts at:      0      12     909 
   Size:         12     897
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     111     909 
   Size:        111     798
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     141     909 
   Size:        141     768
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     610     909 
   Size:        144     466     299

  Fragments arranged by size: 

                 466     299     144
 
Bpu10I CC'TnA_GC

Cuts at:      0     890     909 
   Size:        890      19
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     653     909 
   Size:        653     256
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     909 
   Size:        130     779
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     793     909 
   Size:        793     116
 
Bsp1286I G_dGCh'C

Cuts at:      0      12     909 
   Size:         12     897
 
BsrDI GCAATG_nn'

Cuts at:      0      75     909 
   Size:         75     834
 
DraI TTT'AAA

Cuts at:      0     428     909 
   Size:        428     481
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     291     610     909 
   Size:        144     147     319     299

  Fragments arranged by size: 

                 319     299     147     144
 
EcoRI G'AATT_C

Cuts at:      0      46     909 
   Size:         46     863
 
MslI CAynn'nnrTG

Cuts at:      0     226     683     909 
   Size:        226     457     226

  Fragments arranged by size: 

                 457     226     226
 
NsiI A_TGCA'T

Cuts at:      0     452     909 
   Size:        452     457
 
NspI r_CATG'y

Cuts at:      0     450     792     909 
   Size:        450     342     117

  Fragments arranged by size: 

                 450     342     117
 
PshAI GACnn'nnGTC

Cuts at:      0     673     909 
   Size:        673     236
 
PstI C_TGCA'G

Cuts at:      0     617     909 
   Size:        617     292
 
ScaI AGT'ACT

Cuts at:      0      83     909 
   Size:         83     826
 
SfcI C'TryA_G

Cuts at:      0     613     909 
   Size:        613     296
 
SspI AAT'ATT

Cuts at:      0     185     909 
   Size:        185     724
 
StuI AGG'CCT

Cuts at:      0     871     909 
   Size:        871      38
 
StyI C'CwwG_G

Cuts at:      0     410     671     909 
   Size:        410     261     238

  Fragments arranged by size: 

                 410     261     238
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     485     522     909 
   Size:        485      37     387

  Fragments arranged by size: 

                 485     387      37
 
TatI w'GTAC_w

Cuts at:      0      81     909 
   Size:         81     828
 
Tth111I GACn'n_nGTC

Cuts at:      0     103     909 
   Size:        103     806
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI     BclI    BfrBI Bme1580I     BmrI     BplI
     BpmI   Bpu10I     BsaI    BseRI    BsmBI Bsp1286I    BsrDI     DraI
  Eco57MI    EcoRI     MslI     NsiI     NspI    PshAI     PstI     ScaI
     SfcI     SspI     StuI     StyI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI
    BmgBI     BmtI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI