Restriction map for
pFN21AE5324
(Linear) (Six-base) MAPSORT of: KIEE5324.seq Check: 8552 from: 1 to: 897
KIEE5324.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AccI GT'mk_AC
Cuts at: 0 586 897
Size: 586 311
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 682 897
Size: 373 309 215
Fragments arranged by size:
373 309 215
AfeI AGC'GCT
Cuts at: 0 9 897
Size: 9 888
AflII C'TTAA_G
Cuts at: 0 195 897
Size: 195 702
AlwNI CAG_nnn'CTG
Cuts at: 0 180 501 897
Size: 180 321 396
Fragments arranged by size:
396 321 180
ApaI G_GGCC'C
Cuts at: 0 895 897
Size: 895 2
ApoI r'AATT_y
Cuts at: 0 46 112 897
Size: 46 66 785
Fragments arranged by size:
785 66 46
AvrII C'CTAG_G
Cuts at: 0 15 829 897
Size: 15 814 68
Fragments arranged by size:
814 68 15
BanI G'GyrC_C
Cuts at: 0 383 897
Size: 383 514
BanII G_rGCy'C
Cuts at: 0 895 897
Size: 895 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 753 871 897
Size: 294 459 118 26
Fragments arranged by size:
459 294 118 26
BciVI GTATCCnnnnn_n'
Cuts at: 0 488 897
Size: 488 409
BclI T'GATC_A
Cuts at: 0 182 815 897
Size: 182 633 82
Fragments arranged by size:
633 182 82
BfrBI ATG'CAT
Cuts at: 0 508 897
Size: 508 389
BglI GCCn_nnn'nGGC
Cuts at: 0 155 392 897
Size: 155 237 505
Fragments arranged by size:
505 237 155
BlpI GC'TnA_GC
Cuts at: 0 4 897
Size: 4 893
Bme1580I G_kGCm'C
Cuts at: 0 895 897
Size: 895 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 897
Size: 108 789
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 726 756 897
Size: 726 30 141
Fragments arranged by size:
726 141 30
BseYI C'CCAG_C
Cuts at: 0 887 897
Size: 887 10
BsiEI CG_ry'CG
Cuts at: 0 333 897
Size: 333 564
BsiHKAI G_wGCw'C
Cuts at: 0 879 897
Size: 879 18
BsmI GAATG_Cn'
Cuts at: 0 470 897
Size: 470 427
Bsp1286I G_dGCh'C
Cuts at: 0 879 895 897
Size: 879 16 2
Fragments arranged by size:
879 16 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 619 897
Size: 619 278
BstYI r'GATC_y
Cuts at: 0 854 897
Size: 854 43
BstZ17I GTA'TAC
Cuts at: 0 587 897
Size: 587 310
BtsI GCAGTG_nn'
Cuts at: 0 44 181 653 897
Size: 44 137 472 244
Fragments arranged by size:
472 244 137 44
EaeI y'GGCC_r
Cuts at: 0 707 897
Size: 707 190
EarI CTCTTCn'nnn_
Cuts at: 0 803 897
Size: 803 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 373 682 897
Size: 108 265 309 215
Fragments arranged by size:
309 265 215 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 455 897
Size: 455 442
EcoO109I rG'GnC_Cy
Cuts at: 0 255 362 891 897
Size: 255 107 529 6
Fragments arranged by size:
529 255 107 6
HaeII r_GCGC'y
Cuts at: 0 11 897
Size: 11 886
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 89 121 315 347 726 758 897
Size: 89 32 194 32 379 32 139
Fragments arranged by size:
379 194 139 89 32 32 32
HindIII A'AGCT_T
Cuts at: 0 198 897
Size: 198 699
MscI TGG'CCA
Cuts at: 0 709 897
Size: 709 188
MspA1I CmG'CkG
Cuts at: 0 619 897
Size: 619 278
NsiI A_TGCA'T
Cuts at: 0 510 897
Size: 510 387
PflMI CCAn_nnn'nTGG
Cuts at: 0 278 897
Size: 278 619
PpuMI rG'GwC_Cy
Cuts at: 0 255 897
Size: 255 642
PsiI TTA'TAA
Cuts at: 0 403 897
Size: 403 494
PspOMI G'GGCC_C
Cuts at: 0 891 897
Size: 891 6
PstI C_TGCA'G
Cuts at: 0 176 897
Size: 176 721
PvuI CG_AT'CG
Cuts at: 0 333 897
Size: 333 564
PvuII CAG'CTG
Cuts at: 0 619 897
Size: 619 278
SexAI A'CCwGG_T
Cuts at: 0 692 897
Size: 692 205
SfcI C'TryA_G
Cuts at: 0 172 897
Size: 172 725
SmlI C'TyrA_G
Cuts at: 0 195 897
Size: 195 702
StyI C'CwwG_G
Cuts at: 0 15 456 641 668 829 897
Size: 15 441 185 27 161 68
Fragments arranged by size:
441 185 161 68 27 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 897
Size: 65 32 800
Fragments arranged by size:
800 65 32
Enzymes that do cut:
AccI AcuI AfeI AflII AlwNI ApaI ApoI AvrII
BanI BanII BbsI BciVI BclI BfrBI BglI BlpI
Bme1580I BpmI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BstXI BstYI BstZ17I BtsI EaeI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII MscI MspA1I NsiI PflMI
PpuMI PsiI PspOMI PstI PvuI PvuII SexAI SfcI
SmlI StyI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI BaeI
BamHI BbeI BbvCI BcgI BglII BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BsgI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PspXI PsrI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI