(Linear) (Six-base) MAPSORT of: KIEE5323.seq  Check: 5066  from: 1  to: 849



KIEE5323.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701     849 
   Size:        701     148
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     336     368     849 
   Size:        336      32     481

  Fragments arranged by size: 

                 481     336      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     373     849 
   Size:        373     476
 
ApoI r'AATT_y

Cuts at:      0     151     263     849 
   Size:        151     112     586

  Fragments arranged by size: 

                 586     151     112
 
BamHI G'GATC_C

Cuts at:      0     344     849 
   Size:        344     505
 
BbvCI CC'TCA_GC

Cuts at:      0     596     849 
   Size:        596     253
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     222     849 
   Size:        222     627
 
BclI T'GATC_A

Cuts at:      0     738     849 
   Size:        738     111
 
BlpI GC'TnA_GC

Cuts at:      0     515     849 
   Size:        515     334
 
Bme1580I G_kGCm'C

Cuts at:      0      72     849 
   Size:         72     777
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     156     849 
   Size:        156     693
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     611     849 
   Size:        611     238
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     431     849 
   Size:        227     204     418

  Fragments arranged by size: 

                 418     227     204
 
Bpu10I CC'TnA_GC

Cuts at:      0     596     815     849 
   Size:        596     219      34

  Fragments arranged by size: 

                 596     219      34
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     683     849 
   Size:        683     166
 
BsmI GAATG_Cn'

Cuts at:      0     839     849 
   Size:        839      10
 
Bsp1286I G_dGCh'C

Cuts at:      0      72     849 
   Size:         72     777
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     543     638     849 
   Size:        543      95     211

  Fragments arranged by size: 

                 543     211      95
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     473     578     849 
   Size:        473     105     271

  Fragments arranged by size: 

                 473     271     105
 
BstYI r'GATC_y

Cuts at:      0     344     849 
   Size:        344     505
 
BtsI GCAGTG_nn'

Cuts at:      0     589     849 
   Size:        589     260
 
EarI CTCTTCn'nnn_

Cuts at:      0     615     849 
   Size:        615     234
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     431     701     849 
   Size:        227     204     270     148

  Fragments arranged by size: 

                 270     227     204     148
 
EcoRV GAT'ATC

Cuts at:      0     406     849 
   Size:        406     443
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       1      33      81     113     498     530     849 
   Size:          1      32      48      32     385      32     319

  Fragments arranged by size: 

                 385     319      48      32      32      32       1
 
MslI CAynn'nnrTG

Cuts at:      0     425     790     849 
   Size:        425     365      59

  Fragments arranged by size: 

                 425     365      59
 
MspA1I CmG'CkG

Cuts at:      0     373     849 
   Size:        373     476
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      79     373     849 
   Size:         79     294     476

  Fragments arranged by size: 

                 476     294      79
 
PsiI TTA'TAA

Cuts at:      0     293     849 
   Size:        293     556
 
PvuII CAG'CTG

Cuts at:      0     373     849 
   Size:        373     476
 
SexAI A'CCwGG_T

Cuts at:      0     764     849 
   Size:        764      85
 
StyI C'CwwG_G

Cuts at:      0     472     849 
   Size:        472     377
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     450     849 
   Size:        450     399
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI    BamHI    BbvCI    BciVI     BclI
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BseRI     BsmI
 Bsp1286I    BspMI    BstXI    BstYI     BtsI     EarI  Eco57MI    EcoRV
    Hin4I     MslI   MspA1I    PflMI     PsiI    PvuII    SexAI     StyI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
     BcgI    BfrBI     BglI    BglII    BmgBI     BmtI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI