Restriction map for
pFN21AE5322
(Linear) (Six-base) MAPSORT of: KIEE5322.seq Check: 831 from: 1 to: 792
KIEE5322.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 330 792
Size: 330 462
AccI GT'mk_AC
Cuts at: 0 86 792
Size: 86 706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 792
Size: 91 701
AflIII A'CryG_T
Cuts at: 0 155 792
Size: 155 637
AvaI C'yCGr_G
Cuts at: 0 126 223 398 593 625 792
Size: 126 97 175 195 32 167
Fragments arranged by size:
195 175 167 126 97 32
BamHI G'GATC_C
Cuts at: 0 557 792
Size: 557 235
BanII G_rGCy'C
Cuts at: 0 224 569 649 792
Size: 224 345 80 143
Fragments arranged by size:
345 224 143 80
BglI GCCn_nnn'nGGC
Cuts at: 0 10 290 792
Size: 10 280 502
Fragments arranged by size:
502 280 10
BlpI GC'TnA_GC
Cuts at: 0 208 792
Size: 208 584
BmgBI CAC'GTC
Cuts at: 0 81 156 792
Size: 81 75 636
Fragments arranged by size:
636 81 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 373 535 792
Size: 28 345 162 257
Fragments arranged by size:
345 257 162 28
Bpu10I CC'TnA_GC
Cuts at: 0 218 792
Size: 218 574
BsaI GGTCTCn'nnnn_
Cuts at: 0 107 792
Size: 107 685
BsaHI Gr'CG_yC
Cuts at: 0 293 792
Size: 293 499
BsaWI w'CCGG_w
Cuts at: 0 554 688 792
Size: 554 134 104
Fragments arranged by size:
554 134 104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 792
Size: 574 30 188
Fragments arranged by size:
574 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 416 471 657 792
Size: 416 55 186 135
Fragments arranged by size:
416 186 135 55
BseYI C'CCAG_C
Cuts at: 0 420 615 792
Size: 420 195 177
Fragments arranged by size:
420 195 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 792
Size: 352 440
BsiEI CG_ry'CG
Cuts at: 0 468 792
Size: 468 324
BsiHKAI G_wGCw'C
Cuts at: 0 209 224 602 792
Size: 209 15 378 190
Fragments arranged by size:
378 209 190 15
Bsp1286I G_dGCh'C
Cuts at: 0 209 224 569 602 649 792
Size: 209 15 345 33 47 143
Fragments arranged by size:
345 209 143 47 33 15
BspEI T'CCGG_A
Cuts at: 0 554 688 792
Size: 554 134 104
Fragments arranged by size:
554 134 104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 330 539 792
Size: 314 16 209 253
Fragments arranged by size:
314 253 209 16
BstYI r'GATC_y
Cuts at: 0 380 557 792
Size: 380 177 235
Fragments arranged by size:
380 235 177
Bsu36I CC'TnA_GG
Cuts at: 0 454 699 792
Size: 454 245 93
Fragments arranged by size:
454 245 93
BtgI C'CryG_G
Cuts at: 0 494 792
Size: 494 298
EaeI y'GGCC_r
Cuts at: 0 323 792
Size: 323 469
EcoICRI GAG'CTC
Cuts at: 0 222 792
Size: 222 570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 91 373 535 792
Size: 28 63 282 162 257
Fragments arranged by size:
282 257 162 63 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 370 792
Size: 370 422
EcoO109I rG'GnC_Cy
Cuts at: 0 228 479 736 792
Size: 228 251 257 56
Fragments arranged by size:
257 251 228 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 574 587 606 619 792
Size: 574 13 19 13 173
Fragments arranged by size:
574 173 19 13 13
HincII GTy'rAC
Cuts at: 0 153 792
Size: 153 639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 792
Size: 408 384
MscI TGG'CCA
Cuts at: 0 325 792
Size: 325 467
MslI CAynn'nnrTG
Cuts at: 0 493 792
Size: 493 299
MspA1I CmG'CkG
Cuts at: 0 523 792
Size: 523 269
NspI r_CATG'y
Cuts at: 0 674 792
Size: 674 118
PpuMI rG'GwC_Cy
Cuts at: 0 228 792
Size: 228 564
PspXI vC'TCGA_Gb
Cuts at: 0 223 398 792
Size: 223 175 394
Fragments arranged by size:
394 223 175
PstI C_TGCA'G
Cuts at: 0 137 341 395 550 792
Size: 137 204 54 155 242
Fragments arranged by size:
242 204 155 137 54
SacI G_AGCT'C
Cuts at: 0 224 792
Size: 224 568
SfcI C'TryA_G
Cuts at: 0 133 337 391 546 792
Size: 133 204 54 155 246
Fragments arranged by size:
246 204 155 133 54
SmaI CCC'GGG
Cuts at: 0 627 792
Size: 627 165
SmlI C'TyrA_G
Cuts at: 0 223 398 792
Size: 223 175 394
Fragments arranged by size:
394 223 175
SphI G_CATG'C
Cuts at: 0 674 792
Size: 674 118
StyI C'CwwG_G
Cuts at: 0 288 751 792
Size: 288 463 41
Fragments arranged by size:
463 288 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 648 792
Size: 648 144
Tth111I GACn'n_nGTC
Cuts at: 0 178 792
Size: 178 614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 268 792
Size: 268 524
XhoI C'TCGA_G
Cuts at: 0 223 398 792
Size: 223 175 394
Fragments arranged by size:
394 223 175
XmaI C'CCGG_G
Cuts at: 0 625 792
Size: 625 167
Enzymes that do cut:
AarI AccI AcuI AflIII AvaI BamHI BanII BglI
BlpI BmgBI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BstYI Bsu36I BtgI EaeI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HincII MmeI MscI MslI MspA1I NspI PpuMI
PspXI PstI SacI SfcI SmaI SmlI SphI StyI
TaqII Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII Bme1580I BmrI BmtI BplI
BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TatI TstI XbaI XmnI ZraI