Restriction map for
pFN21AE5316
(Linear) (Six-base) MAPSORT of: KIEE5316.seq Check: 9516 from: 1 to: 855
KIEE5316.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AatII G_ACGT'C
Cuts at: 0 390 855
Size: 390 465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 185 479 855
Size: 116 69 294 376
Fragments arranged by size:
376 294 116 69
AlwNI CAG_nnn'CTG
Cuts at: 0 106 312 855
Size: 106 206 543
Fragments arranged by size:
543 206 106
BanI G'GyrC_C
Cuts at: 0 222 855
Size: 222 633
BanII G_rGCy'C
Cuts at: 0 359 471 855
Size: 359 112 384
Fragments arranged by size:
384 359 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 574 855
Size: 574 281
BbvCI CC'TCA_GC
Cuts at: 0 503 676 855
Size: 503 173 179
Fragments arranged by size:
503 179 173
BfrBI ATG'CAT
Cuts at: 0 546 855
Size: 546 309
Bpu10I CC'TnA_GC
Cuts at: 0 503 676 855
Size: 503 173 179
Fragments arranged by size:
503 179 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 112 855
Size: 103 9 743
Fragments arranged by size:
743 103 9
BsaHI Gr'CG_yC
Cuts at: 0 387 855
Size: 387 468
BsaWI w'CCGG_w
Cuts at: 0 272 419 855
Size: 272 147 436
Fragments arranged by size:
436 272 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 236 266 855
Size: 236 30 589
Fragments arranged by size:
589 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 52 55 88 130 196 361 364
Size: 49 3 3 33 42 66 165 3
Cuts at: 364 367 493 496 499 648 855
Size: 3 126 3 3 149 207
Fragments arranged by size:
207 165 149 126 66 49 42 33
3 3 3 3 3 3
BsiHKAI G_wGCw'C
Cuts at: 0 359 855
Size: 359 496
BsmBI CGTCTCn'nnnn_
Cuts at: 0 378 855
Size: 378 477
Bsp1286I G_dGCh'C
Cuts at: 0 359 471 855
Size: 359 112 384
Fragments arranged by size:
384 359 112
BspEI T'CCGG_A
Cuts at: 0 419 855
Size: 419 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 746 855
Size: 746 109
BsrBI CCG'CTC
Cuts at: 0 399 429 441 576 603 855
Size: 399 30 12 135 27 252
Fragments arranged by size:
399 252 135 30 27 12
BstYI r'GATC_y
Cuts at: 0 233 855
Size: 233 622
BtgI C'CryG_G
Cuts at: 0 822 855
Size: 822 33
EaeI y'GGCC_r
Cuts at: 0 647 855
Size: 647 208
EarI CTCTTCn'nnn_
Cuts at: 0 8 20 59 80 140 455 583 855
Size: 8 12 39 21 60 315 128 272
Fragments arranged by size:
315 272 128 60 39 21 12 8
EcoICRI GAG'CTC
Cuts at: 0 357 855
Size: 357 498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 185 479 855
Size: 116 69 294 376
Fragments arranged by size:
376 294 116 69
EcoO109I rG'GnC_Cy
Cuts at: 0 291 855
Size: 291 564
HaeII r_GCGC'y
Cuts at: 0 313 849 855
Size: 313 536 6
Fragments arranged by size:
536 313 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 306 338 855
Size: 306 32 517
Fragments arranged by size:
517 306 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 855
Size: 560 295
MscI TGG'CCA
Cuts at: 0 649 855
Size: 649 206
MslI CAynn'nnrTG
Cuts at: 0 662 855
Size: 662 193
MspA1I CmG'CkG
Cuts at: 0 58 82 675 824 855
Size: 58 24 593 149 31
Fragments arranged by size:
593 149 58 31 24
NsiI A_TGCA'T
Cuts at: 0 548 855
Size: 548 307
PpuMI rG'GwC_Cy
Cuts at: 0 291 855
Size: 291 564
PstI C_TGCA'G
Cuts at: 0 743 855
Size: 743 112
PvuII CAG'CTG
Cuts at: 0 58 675 855
Size: 58 617 180
Fragments arranged by size:
617 180 58
SacI G_AGCT'C
Cuts at: 0 359 855
Size: 359 496
SacII CC_GC'GG
Cuts at: 0 825 855
Size: 825 30
SapI GCTCTTCn'nnn_
Cuts at: 0 59 855
Size: 59 796
SbfI CC_TGCA'GG
Cuts at: 0 743 855
Size: 743 112
SfcI C'TryA_G
Cuts at: 0 739 855
Size: 739 116
StyI C'CwwG_G
Cuts at: 0 689 855
Size: 689 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 855
Size: 127 728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 236 268 855
Size: 236 32 587
Fragments arranged by size:
587 236 32
ZraI GAC'GTC
Cuts at: 0 388 855
Size: 388 467
Enzymes that do cut:
AatII AcuI AlwNI BanI BanII BbsI BbvCI BfrBI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BstYI BtgI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI
MspA1I NsiI PpuMI PstI PvuII SacI SacII SapI
SbfI SfcI StyI TaqII TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaAI BsaBI BseYI BsgI
BsiEI BsiWI BsmI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI XmnI