(Linear) (Six-base) MAPSORT of: KIEE5310.seq  Check: 6892  from: 1  to: 924



KIEE5310.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26      88     924 
   Size:         26      62     836

  Fragments arranged by size: 

                 836      62      26
 
AflII C'TTAA_G

Cuts at:      0     900     924 
   Size:        900      24
 
AleI CACnn'nnGTG

Cuts at:      0     115     924 
   Size:        115     809
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403     924 
   Size:        403     521
 
ApoI r'AATT_y

Cuts at:      0     786     908     924 
   Size:        786     122      16

  Fragments arranged by size: 

                 786     122      16
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     376     409     924 
   Size:        376      33     515

  Fragments arranged by size: 

                 515     376      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      76     924 
   Size:         76     848
 
BclI T'GATC_A

Cuts at:      0     107     924 
   Size:        107     817
 
Bpu10I CC'TnA_GC

Cuts at:      0      62     924 
   Size:         62     862
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     924 
   Size:        311     613
 
BsmI GAATG_Cn'

Cuts at:      0     422     462     924 
   Size:        422      40     462

  Fragments arranged by size: 

                 462     422      40
 
BspHI T'CATG_A

Cuts at:      0     401     924 
   Size:        401     523
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     467     924 
   Size:        467     457
 
BsrDI GCAATG_nn'

Cuts at:      0     356     924 
   Size:        356     568
 
BsrGI T'GTAC_A

Cuts at:      0     176     924 
   Size:        176     748
 
BstYI r'GATC_y

Cuts at:      0     757     924 
   Size:        757     167
 
BtgI C'CryG_G

Cuts at:      0     113     924 
   Size:        113     811
 
BtsI GCAGTG_nn'

Cuts at:      0     385     924 
   Size:        385     539
 
EaeI y'GGCC_r

Cuts at:      0     371     924 
   Size:        371     553
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26      88     924 
   Size:         26      62     836

  Fragments arranged by size: 

                 836      62      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     440     924 
   Size:        440     484
 
HindIII A'AGCT_T

Cuts at:      0     707     924 
   Size:        707     217
 
MscI TGG'CCA

Cuts at:      0     373     924 
   Size:        373     551
 
MslI CAynn'nnrTG

Cuts at:      0     115     172     403     427     924 
   Size:        115      57     231      24     497

  Fragments arranged by size: 

                 497     231     115      57      24
 
MspA1I CmG'CkG

Cuts at:      0     820     924 
   Size:        820     104
 
NdeI CA'TA_TG

Cuts at:      0     376     924 
   Size:        376     548
 
PstI C_TGCA'G

Cuts at:      0     330     392     924 
   Size:        330      62     532

  Fragments arranged by size: 

                 532     330      62
 
PvuII CAG'CTG

Cuts at:      0     820     924 
   Size:        820     104
 
SfcI C'TryA_G

Cuts at:      0     326     388     924 
   Size:        326      62     536

  Fragments arranged by size: 

                 536     326      62
 
SmlI C'TyrA_G

Cuts at:      0     900     924 
   Size:        900      24
 
SspI AAT'ATT

Cuts at:      0     126     924 
   Size:        126     798
 
TatI w'GTAC_w

Cuts at:      0     176     294     924 
   Size:        176     118     630

  Fragments arranged by size: 

                 630     176     118
 
XmnI GAAnn'nnTTC

Cuts at:      0     590     924 
   Size:        590     334
 

 Enzymes that do cut:

     AcuI    AflII     AleI     AlfI     ApoI     BaeI     BbsI     BclI
   Bpu10I     BsgI     BsmI    BspHI    BspMI    BsrDI    BsrGI    BstYI
     BtgI     BtsI     EaeI  Eco57MI EcoO109I  HindIII     MscI     MslI
   MspA1I     NdeI     PstI    PvuII     SfcI     SmlI     SspI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI