Restriction map for
pFN21AE5302
(Linear) (Six-base) MAPSORT of: KIEE5302.seq Check: 8087 from: 1 to: 831
KIEE5302.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 642 831
Size: 642 189
Acc65I G'GTAC_C
Cuts at: 0 47 831
Size: 47 784
AflIII A'CryG_T
Cuts at: 0 347 831
Size: 347 484
AleI CACnn'nnGTG
Cuts at: 0 772 831
Size: 772 59
AlwNI CAG_nnn'CTG
Cuts at: 0 648 814 831
Size: 648 166 17
Fragments arranged by size:
648 166 17
ApaI G_GGCC'C
Cuts at: 0 30 268 831
Size: 30 238 563
Fragments arranged by size:
563 238 30
AvaI C'yCGr_G
Cuts at: 0 29 158 551 831
Size: 29 129 393 280
Fragments arranged by size:
393 280 129 29
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 504 537 831
Size: 504 33 294
Fragments arranged by size:
504 294 33
BanI G'GyrC_C
Cuts at: 0 47 137 484 547 831
Size: 47 90 347 63 284
Fragments arranged by size:
347 284 90 63 47
BanII G_rGCy'C
Cuts at: 0 30 165 268 466 694 767 831
Size: 30 135 103 198 228 73 64
Fragments arranged by size:
228 198 135 103 73 64 30
BbeI G_GCGC'C
Cuts at: 0 551 831
Size: 551 280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 181 215 831
Size: 181 34 616
Fragments arranged by size:
616 181 34
BglI GCCn_nnn'nGGC
Cuts at: 0 34 580 831
Size: 34 546 251
Fragments arranged by size:
546 251 34
Bme1580I G_kGCm'C
Cuts at: 0 30 268 831
Size: 30 238 563
Fragments arranged by size:
563 238 30
BmtI G_CTAG'C
Cuts at: 0 827 831
Size: 827 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 831
Size: 97 734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 831
Size: 586 245
BsaHI Gr'CG_yC
Cuts at: 0 37 548 831
Size: 37 511 283
Fragments arranged by size:
511 283 37
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 601 831
Size: 298 303 230
Fragments arranged by size:
303 298 230
BseYI C'CCAG_C
Cuts at: 0 57 819 831
Size: 57 762 12
Fragments arranged by size:
762 57 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 414 796 831
Size: 127 287 382 35
Fragments arranged by size:
382 287 127 35
BsiEI CG_ry'CG
Cuts at: 0 97 272 831
Size: 97 175 559
Fragments arranged by size:
559 175 97
Bsp1286I G_dGCh'C
Cuts at: 0 30 165 268 466 694 767 831
Size: 30 135 103 198 228 73 64
Fragments arranged by size:
228 198 135 103 73 64 30
BspHI T'CATG_A
Cuts at: 0 524 593 831
Size: 524 69 238
Fragments arranged by size:
524 238 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 35 642 831
Size: 35 607 189
Fragments arranged by size:
607 189 35
BsrBI CCG'CTC
Cuts at: 0 76 171 831
Size: 76 95 660
Fragments arranged by size:
660 95 76
BsrDI GCAATG_nn'
Cuts at: 0 569 831
Size: 569 262
BsrFI r'CCGG_y
Cuts at: 0 481 801 831
Size: 481 320 30
Fragments arranged by size:
481 320 30
BsrGI T'GTAC_A
Cuts at: 0 350 831
Size: 350 481
BssHII G'CGCG_C
Cuts at: 0 210 831
Size: 210 621
BstAPI GCAn_nnn'nTGC
Cuts at: 0 254 437 831
Size: 254 183 394
Fragments arranged by size:
394 254 183
BstEII G'GTnAC_C
Cuts at: 0 375 831
Size: 375 456
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 712 831
Size: 186 526 119
Fragments arranged by size:
526 186 119
Bsu36I CC'TnA_GG
Cuts at: 0 540 831
Size: 540 291
BtgI C'CryG_G
Cuts at: 0 380 668 695 831
Size: 380 288 27 136
Fragments arranged by size:
380 288 136 27
ClaI AT'CG_AT
Cuts at: 0 272 831
Size: 272 559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 552 587 831
Size: 552 35 244
Fragments arranged by size:
552 244 35
DraIII CAC_nnn'GTG
Cuts at: 0 437 831
Size: 437 394
EaeI y'GGCC_r
Cuts at: 0 94 389 831
Size: 94 295 442
Fragments arranged by size:
442 295 94
EagI C'GGCC_G
Cuts at: 0 94 831
Size: 94 737
EarI CTCTTCn'nnn_
Cuts at: 0 316 831
Size: 316 515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 654 831
Size: 654 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 831
Size: 97 734
EcoO109I rG'GnC_Cy
Cuts at: 0 26 61 831
Size: 26 35 770
Fragments arranged by size:
770 35 26
HaeII r_GCGC'y
Cuts at: 0 551 802 831
Size: 551 251 29
Fragments arranged by size:
551 251 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 211 243 831
Size: 147 32 32 32 588
Fragments arranged by size:
588 147 32 32 32
KasI G'GCGC_C
Cuts at: 0 547 831
Size: 547 284
KpnI G_GTAC'C
Cuts at: 0 51 831
Size: 51 780
MscI TGG'CCA
Cuts at: 0 391 831
Size: 391 440
MslI CAynn'nnrTG
Cuts at: 0 184 346 772 831
Size: 184 162 426 59
Fragments arranged by size:
426 184 162 59
MspA1I CmG'CkG
Cuts at: 0 93 105 135 382 831
Size: 93 12 30 247 449
Fragments arranged by size:
449 247 93 30 12
NaeI GCC'GGC
Cuts at: 0 483 803 831
Size: 483 320 28
Fragments arranged by size:
483 320 28
NarI GG'CG_CC
Cuts at: 0 548 831
Size: 548 283
NcoI C'CATG_G
Cuts at: 0 668 695 831
Size: 668 27 136
Fragments arranged by size:
668 136 27
NgoMIV G'CCGG_C
Cuts at: 0 481 801 831
Size: 481 320 30
Fragments arranged by size:
481 320 30
NheI G'CTAG_C
Cuts at: 0 823 831
Size: 823 8
NotI GC'GGCC_GC
Cuts at: 0 94 831
Size: 94 737
NspI r_CATG'y
Cuts at: 0 351 498 831
Size: 351 147 333
Fragments arranged by size:
351 333 147
PciI A'CATG_T
Cuts at: 0 347 831
Size: 347 484
PspOMI G'GGCC_C
Cuts at: 0 26 264 831
Size: 26 238 567
Fragments arranged by size:
567 238 26
PstI C_TGCA'G
Cuts at: 0 134 653 831
Size: 134 519 178
Fragments arranged by size:
519 178 134
PvuI CG_AT'CG
Cuts at: 0 272 831
Size: 272 559
SacII CC_GC'GG
Cuts at: 0 383 831
Size: 383 448
SbfI CC_TGCA'GG
Cuts at: 0 653 831
Size: 653 178
SexAI A'CCwGG_T
Cuts at: 0 433 831
Size: 433 398
SfcI C'TryA_G
Cuts at: 0 130 649 788 831
Size: 130 519 139 43
Fragments arranged by size:
519 139 130 43
SfoI GGC'GCC
Cuts at: 0 549 831
Size: 549 282
SmaI CCC'GGG
Cuts at: 0 31 160 553 831
Size: 31 129 393 278
Fragments arranged by size:
393 278 129 31
SmlI C'TyrA_G
Cuts at: 0 601 831
Size: 601 230
SphI G_CATG'C
Cuts at: 0 498 831
Size: 498 333
SspI AAT'ATT
Cuts at: 0 14 831
Size: 14 817
StyI C'CwwG_G
Cuts at: 0 668 695 831
Size: 668 27 136
Fragments arranged by size:
668 136 27
TatI w'GTAC_w
Cuts at: 0 350 410 831
Size: 350 60 421
Fragments arranged by size:
421 350 60
XmaI C'CCGG_G
Cuts at: 0 29 158 551 831
Size: 29 129 393 280
Fragments arranged by size:
393 280 129 29
Enzymes that do cut:
AarI Acc65I AflIII AleI AlwNI ApaI AvaI BaeI
BanI BanII BbeI BcgI BglI Bme1580I BmtI BpmI
BpuEI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII
BstXI Bsu36I BtgI ClaI CspCI DraIII EaeI EagI
EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI KpnI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NspI PciI PspOMI PstI PvuI SacII SbfI
SexAI SfcI SfoI SmaI SmlI SphI SspI StyI
TatI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BplI Bpu10I BsaI BsaAI BsaBI BsaWI
BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BssSI BstBI
BstYI BstZ17I BtgZI BtsI DraI DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI NdeI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuII RsrII SacI SalI SanDI
SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmnI ZraI