(Linear) (Six-base) MAPSORT of: KIEE5296.seq  Check: 7807  from: 1  to: 522



KIEE5296.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:54  ..

AfeI AGC'GCT

Cuts at:      0       8     522 
   Size:          8     514
 
AflIII A'CryG_T

Cuts at:      0     386     522 
   Size:        386     136
 
AlwNI CAG_nnn'CTG

Cuts at:      0     219     522 
   Size:        219     303
 
AvaI C'yCGr_G

Cuts at:      0      69     263     522 
   Size:         69     194     259

  Fragments arranged by size: 

                 259     194      69
 
BglII A'GATC_T

Cuts at:      0     363     522 
   Size:        363     159
 
BlpI GC'TnA_GC

Cuts at:      0     212     370     522 
   Size:        212     158     152

  Fragments arranged by size: 

                 212     158     152
 
Bpu10I CC'TnA_GC

Cuts at:      0     239     522 
   Size:        239     283
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     522 
   Size:        169     353
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     522 
   Size:        118     404
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     522 
   Size:         27     495
 
BsrDI GCAATG_nn'

Cuts at:      0     377     522 
   Size:        377     145
 
BssSI C'ACGA_G

Cuts at:      0     110     522 
   Size:        110     412
 
BstYI r'GATC_y

Cuts at:      0     363     522 
   Size:        363     159
 
Bsu36I CC'TnA_GG

Cuts at:      0     101     522 
   Size:        101     421
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     313     522 
   Size:        313     209
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     103     522 
   Size:        103     419
 
HaeII r_GCGC'y

Cuts at:      0      10     144     522 
   Size:         10     134     378

  Fragments arranged by size: 

                 378     134      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372     522 
   Size:        340      32     150

  Fragments arranged by size: 

                 340     150      32
 
HincII GTy'rAC

Cuts at:      0     315     522 
   Size:        315     207
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     522 
   Size:        367     155
 
MslI CAynn'nnrTG

Cuts at:      0     157     522 
   Size:        157     365
 
NspI r_CATG'y

Cuts at:      0     390     411     522 
   Size:        390      21     111

  Fragments arranged by size: 

                 390     111      21
 
PciI A'CATG_T

Cuts at:      0     386     522 
   Size:        386     136
 
PstI C_TGCA'G

Cuts at:      0     210     522 
   Size:        210     312
 
SfcI C'TryA_G

Cuts at:      0     206     522 
   Size:        206     316
 
SmlI C'TyrA_G

Cuts at:      0      69     184     522 
   Size:         69     115     338

  Fragments arranged by size: 

                 338     115      69
 
XhoI C'TCGA_G

Cuts at:      0      69     522 
   Size:         69     453
 
XmnI GAAnn'nnTTC

Cuts at:      0     201     522 
   Size:        201     321
 

 Enzymes that do cut:

     AfeI   AflIII    AlwNI     AvaI    BglII     BlpI   Bpu10I    BpuEI
    BseRI     BsgI    BsrDI    BssSI    BstYI   Bsu36I    BtgZI     FalI
    HaeII    Hin4I   HincII     MmeI     MslI     NspI     PciI     PstI
     SfcI     SmlI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI