Restriction map for
pFN21AE5295
(Linear) (Six-base) MAPSORT of: KIEE5295.seq Check: 1248 from: 1 to: 612
KIEE5295.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 477 612
Size: 477 135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 292 612
Size: 248 44 320
Fragments arranged by size:
320 248 44
AvaI C'yCGr_G
Cuts at: 0 392 612
Size: 392 220
BamHI G'GATC_C
Cuts at: 0 509 612
Size: 509 103
BanII G_rGCy'C
Cuts at: 0 391 612
Size: 391 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 612
Size: 27 34 551
Fragments arranged by size:
551 34 27
BglI GCCn_nnn'nGGC
Cuts at: 0 183 612
Size: 183 429
BlpI GC'TnA_GC
Cuts at: 0 187 612
Size: 187 425
Bme1580I G_kGCm'C
Cuts at: 0 113 612
Size: 113 499
BmrI ACTGGGnnnn_n'
Cuts at: 0 121 368 612
Size: 121 247 244
Fragments arranged by size:
247 244 121
BmtI G_CTAG'C
Cuts at: 0 562 612
Size: 562 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 350 612
Size: 350 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 612
Size: 345 267
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 582 598 612
Size: 133 449 16 14
Fragments arranged by size:
449 133 16 14
BseYI C'CCAG_C
Cuts at: 0 517 535 612
Size: 517 18 77
Fragments arranged by size:
517 77 18
BsiHKAI G_wGCw'C
Cuts at: 0 381 612
Size: 381 231
Bsp1286I G_dGCh'C
Cuts at: 0 113 381 391 612
Size: 113 268 10 221
Fragments arranged by size:
268 221 113 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 477 612
Size: 477 135
BsrFI r'CCGG_y
Cuts at: 0 10 612
Size: 10 602
BstYI r'GATC_y
Cuts at: 0 509 612
Size: 509 103
Bsu36I CC'TnA_GG
Cuts at: 0 194 612
Size: 194 418
BtsI GCAGTG_nn'
Cuts at: 0 200 612
Size: 200 412
EarI CTCTTCn'nnn_
Cuts at: 0 280 350 488 575 612
Size: 280 70 138 87 37
Fragments arranged by size:
280 138 87 70 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 292 612
Size: 248 44 320
Fragments arranged by size:
320 248 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 327 612
Size: 327 285
EcoO109I rG'GnC_Cy
Cuts at: 0 71 123 612
Size: 71 52 489
Fragments arranged by size:
489 71 52
HaeII r_GCGC'y
Cuts at: 0 577 612
Size: 577 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 612
Size: 331 32 249
Fragments arranged by size:
331 249 32
HincII GTy'rAC
Cuts at: 0 53 612
Size: 53 559
MspA1I CmG'CkG
Cuts at: 0 87 409 433 507 612
Size: 87 322 24 74 105
Fragments arranged by size:
322 105 87 74 24
NaeI GCC'GGC
Cuts at: 0 12 612
Size: 12 600
NgoMIV G'CCGG_C
Cuts at: 0 10 612
Size: 10 602
NheI G'CTAG_C
Cuts at: 0 558 612
Size: 558 54
PasI CC'CwG_GG
Cuts at: 0 181 612
Size: 181 431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 612
Size: 230 32 350
Fragments arranged by size:
350 230 32
PpuMI rG'GwC_Cy
Cuts at: 0 71 123 612
Size: 71 52 489
Fragments arranged by size:
489 71 52
PstI C_TGCA'G
Cuts at: 0 503 612
Size: 503 109
PvuII CAG'CTG
Cuts at: 0 409 433 612
Size: 409 24 179
Fragments arranged by size:
409 179 24
SapI GCTCTTCn'nnn_
Cuts at: 0 488 612
Size: 488 124
SfcI C'TryA_G
Cuts at: 0 499 612
Size: 499 113
SmlI C'TyrA_G
Cuts at: 0 324 612
Size: 324 288
StuI AGG'CCT
Cuts at: 0 225 245 322 608 612
Size: 225 20 77 286 4
Fragments arranged by size:
286 225 77 20 4
XmnI GAAnn'nnTTC
Cuts at: 0 154 612
Size: 154 458
Enzymes that do cut:
AarI AcuI AvaI BamHI BanII BcgI BglI BlpI
Bme1580I BmrI BmtI BplI BpuEI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrFI BstYI Bsu36I BtsI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII MspA1I NaeI NgoMIV
NheI PasI PpiI PpuMI PstI PvuII SapI SfcI
SmlI StuI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI
BbvCI BciVI BclI BfrBI BglII BmgBI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NarI NcoI NdeI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI