Restriction map for
pFN21AE5294
(Linear) (Six-base) MAPSORT of: KIEE5294.seq Check: 230 from: 1 to: 663
KIEE5294.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AatII G_ACGT'C
Cuts at: 0 401 663
Size: 401 262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 346 663
Size: 36 310 317
Fragments arranged by size:
317 310 36
AleI CACnn'nnGTG
Cuts at: 0 373 663
Size: 373 290
AlwNI CAG_nnn'CTG
Cuts at: 0 598 663
Size: 598 65
ApoI r'AATT_y
Cuts at: 0 27 232 654 663
Size: 27 205 422 9
Fragments arranged by size:
422 205 27 9
AvaI C'yCGr_G
Cuts at: 0 227 663
Size: 227 436
BanI G'GyrC_C
Cuts at: 0 212 663
Size: 212 451
BanII G_rGCy'C
Cuts at: 0 296 663
Size: 296 367
BbsI GAAGACnn'nnnn_
Cuts at: 0 446 663
Size: 446 217
BlpI GC'TnA_GC
Cuts at: 0 187 663
Size: 187 476
Bpu10I CC'TnA_GC
Cuts at: 0 290 663
Size: 290 373
BsaBI GATnn'nnATC
Cuts at: 0 338 663
Size: 338 325
BsaHI Gr'CG_yC
Cuts at: 0 398 663
Size: 398 265
BsaWI w'CCGG_w
Cuts at: 0 248 449 663
Size: 248 201 214
Fragments arranged by size:
248 214 201
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 663
Size: 412 30 221
Fragments arranged by size:
412 221 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 663
Size: 433 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 663
Size: 535 128
BsiHKAI G_wGCw'C
Cuts at: 0 193 296 663
Size: 193 103 367
Fragments arranged by size:
367 193 103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 389 663
Size: 389 274
Bsp1286I G_dGCh'C
Cuts at: 0 193 296 663
Size: 193 103 367
Fragments arranged by size:
367 193 103
BspEI T'CCGG_A
Cuts at: 0 248 663
Size: 248 415
BspHI T'CATG_A
Cuts at: 0 330 663
Size: 330 333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 610 663
Size: 610 53
BsrDI GCAATG_nn'
Cuts at: 0 621 663
Size: 621 42
BsrFI r'CCGG_y
Cuts at: 0 256 663
Size: 256 407
BsrGI T'GTAC_A
Cuts at: 0 71 663
Size: 71 592
BssSI C'ACGA_G
Cuts at: 0 45 663
Size: 45 618
BstXI CCAn_nnnn'nTGG
Cuts at: 0 375 663
Size: 375 288
EaeI y'GGCC_r
Cuts at: 0 269 663
Size: 269 394
EcoICRI GAG'CTC
Cuts at: 0 294 663
Size: 294 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 346 663
Size: 36 310 317
Fragments arranged by size:
317 310 36
EcoO109I rG'GnC_Cy
Cuts at: 0 243 663
Size: 243 420
EcoRI G'AATT_C
Cuts at: 0 232 663
Size: 232 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 663
Size: 292 32 339
Fragments arranged by size:
339 292 32
HincII GTy'rAC
Cuts at: 0 431 590 663
Size: 431 159 73
Fragments arranged by size:
431 159 73
HindIII A'AGCT_T
Cuts at: 0 382 663
Size: 382 281
HpaI GTT'AAC
Cuts at: 0 590 663
Size: 590 73
MfeI C'AATT_G
Cuts at: 0 434 663
Size: 434 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 369 663
Size: 202 167 294
Fragments arranged by size:
294 202 167
MslI CAynn'nnrTG
Cuts at: 0 79 373 663
Size: 79 294 290
Fragments arranged by size:
294 290 79
NspI r_CATG'y
Cuts at: 0 168 663
Size: 168 495
PfoI T'CCnGG_A
Cuts at: 0 157 663
Size: 157 506
PstI C_TGCA'G
Cuts at: 0 120 465 663
Size: 120 345 198
Fragments arranged by size:
345 198 120
SacI G_AGCT'C
Cuts at: 0 296 663
Size: 296 367
SfcI C'TryA_G
Cuts at: 0 116 461 663
Size: 116 345 202
Fragments arranged by size:
345 202 116
StyI C'CwwG_G
Cuts at: 0 194 216 663
Size: 194 22 447
Fragments arranged by size:
447 194 22
TatI w'GTAC_w
Cuts at: 0 71 663
Size: 71 592
ZraI GAC'GTC
Cuts at: 0 399 663
Size: 399 264
Enzymes that do cut:
AatII AcuI AleI AlwNI ApoI AvaI BanI BanII
BbsI BlpI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstXI EaeI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I HincII HindIII HpaI MfeI MmeI MslI
NspI PfoI PstI SacI SfcI StyI TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BseYI BsiEI BsiWI BsmI
BsrBI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII KasI KpnI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI