(Linear) (Six-base) MAPSORT of: KIEE5292.seq  Check: 205  from: 1  to: 723



KIEE5292.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:54  ..

AflII C'TTAA_G

Cuts at:      0     154     723 
   Size:        154     569
 
AhdI GACnn_n'nnGTC

Cuts at:      0     526     723 
   Size:        526     197
 
AlwNI CAG_nnn'CTG

Cuts at:      0     246     335     723 
   Size:        246      89     388

  Fragments arranged by size: 

                 388     246      89
 
ApoI r'AATT_y

Cuts at:      0     283     723 
   Size:        283     440
 
AvrII C'CTAG_G

Cuts at:      0     654     723 
   Size:        654      69
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      21     326     723 
   Size:         21     305     397

  Fragments arranged by size: 

                 397     305      21
 
BclI T'GATC_A

Cuts at:      0     596     723 
   Size:        596     127
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     593     723 
   Size:        319     274     130

  Fragments arranged by size: 

                 319     274     130
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     264     723 
   Size:        264     459
 
BseYI C'CCAG_C

Cuts at:      0     665     723 
   Size:        665      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     626     723 
   Size:        626      97
 
BstYI r'GATC_y

Cuts at:      0     650     723 
   Size:        650      73
 
Bsu36I CC'TnA_GG

Cuts at:      0     247     723 
   Size:        247     476
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     593     723 
   Size:        319     274     130

  Fragments arranged by size: 

                 319     274     130
 
EcoO109I rG'GnC_Cy

Cuts at:      0     570     723 
   Size:        570     153
 
EcoRV GAT'ATC

Cuts at:      0     161     723 
   Size:        161     562
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     252     723 
   Size:        252     471
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     180     212     512     544     690     722     723 
   Size:        180      32     300      32     146      32       1

  Fragments arranged by size: 

                 300     180     146      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0     535     723 
   Size:        535     188
 
HindIII A'AGCT_T

Cuts at:      0     148     723 
   Size:        148     575
 
MslI CAynn'nnrTG

Cuts at:      0     674     723 
   Size:        674      49
 
MspA1I CmG'CkG

Cuts at:      0     594     723 
   Size:        594     129
 
PpuMI rG'GwC_Cy

Cuts at:      0     570     723 
   Size:        570     153
 
PstI C_TGCA'G

Cuts at:      0     362     437     623     723 
   Size:        362      75     186     100

  Fragments arranged by size: 

                 362     186     100      75
 
PvuII CAG'CTG

Cuts at:      0     594     723 
   Size:        594     129
 
SfcI C'TryA_G

Cuts at:      0     358     433     619     723 
   Size:        358      75     186     104

  Fragments arranged by size: 

                 358     186     104      75
 
SmlI C'TyrA_G

Cuts at:      0     154     723 
   Size:        154     569
 
SspI AAT'ATT

Cuts at:      0     478     723 
   Size:        478     245
 
StyI C'CwwG_G

Cuts at:      0     654     723 
   Size:        654      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     370     723 
   Size:        370     353
 

 Enzymes that do cut:

    AflII     AhdI    AlwNI     ApoI    AvrII     BbsI     BclI     BpmI
    BseRI    BseYI    BspMI    BstYI   Bsu36I  Eco57MI EcoO109I    EcoRV
     FalI    Hin4I   HincII  HindIII     MslI   MspA1I    PpuMI     PstI
    PvuII     SfcI     SmlI     SspI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI