(Linear) (Six-base) MAPSORT of: KIEE5291.seq  Check: 3233  from: 1  to: 732



KIEE5291.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:54  ..

AccI GT'mk_AC

Cuts at:      0      45     152     732 
   Size:         45     107     580

  Fragments arranged by size: 

                 580     107      45
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     732 
   Size:        514     218
 
AflII C'TTAA_G

Cuts at:      0     645     732 
   Size:        645      87
 
AlwNI CAG_nnn'CTG

Cuts at:      0     265     379     732 
   Size:        265     114     353

  Fragments arranged by size: 

                 353     265     114
 
ApoI r'AATT_y

Cuts at:      0     384     732 
   Size:        384     348
 
AseI AT'TA_AT

Cuts at:      0      59     732 
   Size:         59     673
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     418     502     732 
   Size:        418      84     230

  Fragments arranged by size: 

                 418     230      84
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     175     732 
   Size:        119      56     557

  Fragments arranged by size: 

                 557     119      56
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     193     732 
   Size:        193     539
 
BstEII G'GTnAC_C

Cuts at:      0     606     732 
   Size:        606     126
 
BtgI C'CryG_G

Cuts at:      0     170     732 
   Size:        170     562
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     732 
   Size:        514     218
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     182     453     567     732 
   Size:        182     271     114     165

  Fragments arranged by size: 

                 271     182     165     114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     117     149     235     267     732 
   Size:        117      32      86      32     465

  Fragments arranged by size: 

                 465     117      86      32      32
 
HindIII A'AGCT_T

Cuts at:      0     465     732 
   Size:        465     267
 
MfeI C'AATT_G

Cuts at:      0       7     732 
   Size:          7     725
 
NcoI C'CATG_G

Cuts at:      0     170     732 
   Size:        170     562
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     473     505     732 
   Size:        473      32     227

  Fragments arranged by size: 

                 473     227      32
 
SfcI C'TryA_G

Cuts at:      0      46     732 
   Size:         46     686
 
SmlI C'TyrA_G

Cuts at:      0     134     190     645     732 
   Size:        134      56     455      87

  Fragments arranged by size: 

                 455     134      87      56
 
StyI C'CwwG_G

Cuts at:      0     170     732 
   Size:        170     562
 
TatI w'GTAC_w

Cuts at:      0     160     732 
   Size:        160     572
 

 Enzymes that do cut:

     AccI     AcuI    AflII    AlwNI     ApoI     AseI     BbsI    BpuEI
     BsaI   BstEII     BtgI  Eco57MI     FalI    Hin4I  HindIII     MfeI
     NcoI     PpiI     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI